Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition
- Autores
- Muñoz, Nacira Belen; Rodriguez, Marianela; Robert, German; Lascano, Hernan Ramiro
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–), root hair death and sodium/potassium ion content. We also analysed whether this short-term salt stress influenced later formation of crown and noncrown nodules, determining the number and weight of nodules. The negative effect of salt stress on these characters was attenuated by the addition of 5 mM CaCl2. We also analysed the expression of pathogenesis-related proteins (PRP) genes PR-1, PR-2, PR-3, and four isoforms of PR-5. The expression of PR-2 increased under saline conditions and decreased in osmotic treatment and saline treatment supplemented with calcium in the presence of the symbiont. The changes in PR-2 expression levels, together with the death of root hairs provide a possible mechanism for the inhibition of infection by the symbiont under salinity, and suggests a possible overlap with responses to plant pathogens.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Muñoz, Nacira Belen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina
Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina - Fuente
- Functional Plant Biology 41 (1) : 96-105 ( 2013)
- Materia
-
Root Nodulation
Osmotic Stress
Estrés Osmótico
Nodulación
Bradyrhizobium japonicum
Ionic Homeostasis
Nodulation
Pathogenic-Related Proteins
Symbiotic Interaction
Estrés Salino - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/10933
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Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium additionMuñoz, Nacira BelenRodriguez, MarianelaRobert, GermanLascano, Hernan RamiroRoot NodulationOsmotic StressEstrés OsmóticoNodulaciónBradyrhizobium japonicumIonic HomeostasisNodulationPathogenic-Related ProteinsSymbiotic InteractionEstrés SalinoThe short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–), root hair death and sodium/potassium ion content. We also analysed whether this short-term salt stress influenced later formation of crown and noncrown nodules, determining the number and weight of nodules. The negative effect of salt stress on these characters was attenuated by the addition of 5 mM CaCl2. We also analysed the expression of pathogenesis-related proteins (PRP) genes PR-1, PR-2, PR-3, and four isoforms of PR-5. The expression of PR-2 increased under saline conditions and decreased in osmotic treatment and saline treatment supplemented with calcium in the presence of the symbiont. The changes in PR-2 expression levels, together with the death of root hairs provide a possible mechanism for the inhibition of infection by the symbiont under salinity, and suggests a possible overlap with responses to plant pathogens.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Muñoz, Nacira Belen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. ArgentinaFil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaCSIRO Publishing2021-12-17T19:30:20Z2021-12-17T19:30:20Z2013-09-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10933https://www.publish.csiro.au/fp/FP130851445-44081445-4416 (online)https://doi.org/10.1071/FP13085Functional Plant Biology 41 (1) : 96-105 ( 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:25Zoai:localhost:20.500.12123/10933instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:26.272INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
title |
Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
spellingShingle |
Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition Muñoz, Nacira Belen Root Nodulation Osmotic Stress Estrés Osmótico Nodulación Bradyrhizobium japonicum Ionic Homeostasis Nodulation Pathogenic-Related Proteins Symbiotic Interaction Estrés Salino |
title_short |
Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
title_full |
Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
title_fullStr |
Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
title_full_unstemmed |
Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
title_sort |
Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition |
dc.creator.none.fl_str_mv |
Muñoz, Nacira Belen Rodriguez, Marianela Robert, German Lascano, Hernan Ramiro |
author |
Muñoz, Nacira Belen |
author_facet |
Muñoz, Nacira Belen Rodriguez, Marianela Robert, German Lascano, Hernan Ramiro |
author_role |
author |
author2 |
Rodriguez, Marianela Robert, German Lascano, Hernan Ramiro |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Root Nodulation Osmotic Stress Estrés Osmótico Nodulación Bradyrhizobium japonicum Ionic Homeostasis Nodulation Pathogenic-Related Proteins Symbiotic Interaction Estrés Salino |
topic |
Root Nodulation Osmotic Stress Estrés Osmótico Nodulación Bradyrhizobium japonicum Ionic Homeostasis Nodulation Pathogenic-Related Proteins Symbiotic Interaction Estrés Salino |
dc.description.none.fl_txt_mv |
The short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–), root hair death and sodium/potassium ion content. We also analysed whether this short-term salt stress influenced later formation of crown and noncrown nodules, determining the number and weight of nodules. The negative effect of salt stress on these characters was attenuated by the addition of 5 mM CaCl2. We also analysed the expression of pathogenesis-related proteins (PRP) genes PR-1, PR-2, PR-3, and four isoforms of PR-5. The expression of PR-2 increased under saline conditions and decreased in osmotic treatment and saline treatment supplemented with calcium in the presence of the symbiont. The changes in PR-2 expression levels, together with the death of root hairs provide a possible mechanism for the inhibition of infection by the symbiont under salinity, and suggests a possible overlap with responses to plant pathogens. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Muñoz, Nacira Belen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina |
description |
The short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–), root hair death and sodium/potassium ion content. We also analysed whether this short-term salt stress influenced later formation of crown and noncrown nodules, determining the number and weight of nodules. The negative effect of salt stress on these characters was attenuated by the addition of 5 mM CaCl2. We also analysed the expression of pathogenesis-related proteins (PRP) genes PR-1, PR-2, PR-3, and four isoforms of PR-5. The expression of PR-2 increased under saline conditions and decreased in osmotic treatment and saline treatment supplemented with calcium in the presence of the symbiont. The changes in PR-2 expression levels, together with the death of root hairs provide a possible mechanism for the inhibition of infection by the symbiont under salinity, and suggests a possible overlap with responses to plant pathogens. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-05 2021-12-17T19:30:20Z 2021-12-17T19:30:20Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/10933 https://www.publish.csiro.au/fp/FP13085 1445-4408 1445-4416 (online) https://doi.org/10.1071/FP13085 |
url |
http://hdl.handle.net/20.500.12123/10933 https://www.publish.csiro.au/fp/FP13085 https://doi.org/10.1071/FP13085 |
identifier_str_mv |
1445-4408 1445-4416 (online) |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
CSIRO Publishing |
publisher.none.fl_str_mv |
CSIRO Publishing |
dc.source.none.fl_str_mv |
Functional Plant Biology 41 (1) : 96-105 ( 2013) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619160579997696 |
score |
12.559606 |