Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
- Autores
- Robert, German; Muñoz, Nacira Belen; Melchiorre, Mariana; Sanchez, Federico; Lascano, Hernan Ramiro
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina - Materia
-
Glycine Max
Soja
Bradyrhizobium Japonicum
Estrés Osmótico
Genes
Soybeans
Osmotic Stress
Anti-Apoptotic Gene Ced-9
Saline Stress
Estrés Salino - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/1045
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Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formationRobert, GermanMuñoz, Nacira BelenMelchiorre, MarianaSanchez, FedericoLascano, Hernan RamiroGlycine MaxSojaBradyrhizobium JaponicumEstrés OsmóticoGenesSoybeansOsmotic StressAnti-Apoptotic Gene Ced-9Saline StressEstrés SalinoThe mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina2017-08-25T16:48:20Z2017-08-25T16:48:20Z2014-07-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1045http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable:Robert G, Muñoz N, Melchiorre M, Sánchez F, Lascano R (2014) Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation. PLoS ONE 9(7): e101747. doi:10.1371/journal.pone.01017471932-6203https://doi.org/10.1371/journal.pone.0101747enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuaria2025-09-29T13:44:09Zoai:localhost:20.500.12123/1045instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:09.476INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
title |
Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
spellingShingle |
Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation Robert, German Glycine Max Soja Bradyrhizobium Japonicum Estrés Osmótico Genes Soybeans Osmotic Stress Anti-Apoptotic Gene Ced-9 Saline Stress Estrés Salino |
title_short |
Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
title_full |
Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
title_fullStr |
Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
title_full_unstemmed |
Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
title_sort |
Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation |
dc.creator.none.fl_str_mv |
Robert, German Muñoz, Nacira Belen Melchiorre, Mariana Sanchez, Federico Lascano, Hernan Ramiro |
author |
Robert, German |
author_facet |
Robert, German Muñoz, Nacira Belen Melchiorre, Mariana Sanchez, Federico Lascano, Hernan Ramiro |
author_role |
author |
author2 |
Muñoz, Nacira Belen Melchiorre, Mariana Sanchez, Federico Lascano, Hernan Ramiro |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Glycine Max Soja Bradyrhizobium Japonicum Estrés Osmótico Genes Soybeans Osmotic Stress Anti-Apoptotic Gene Ced-9 Saline Stress Estrés Salino |
topic |
Glycine Max Soja Bradyrhizobium Japonicum Estrés Osmótico Genes Soybeans Osmotic Stress Anti-Apoptotic Gene Ced-9 Saline Stress Estrés Salino |
dc.description.none.fl_txt_mv |
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions. Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina |
description |
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07-27 2017-08-25T16:48:20Z 2017-08-25T16:48:20Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/1045 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable :Robert G, Muñoz N, Melchiorre M, Sánchez F, Lascano R (2014) Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation. PLoS ONE 9(7): e101747. doi:10.1371/journal.pone.0101747 1932-6203 https://doi.org/10.1371/journal.pone.0101747 |
url |
http://hdl.handle.net/20.500.12123/1045 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable https://doi.org/10.1371/journal.pone.0101747 |
identifier_str_mv |
:Robert G, Muñoz N, Melchiorre M, Sánchez F, Lascano R (2014) Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation. PLoS ONE 9(7): e101747. doi:10.1371/journal.pone.0101747 1932-6203 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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