Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation

Autores
Robert, German; Muñoz, Nacira Belen; Melchiorre, Mariana; Sanchez, Federico; Lascano, Hernan Ramiro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Materia
Glycine Max
Soja
Bradyrhizobium Japonicum
Estrés Osmótico
Genes
Soybeans
Osmotic Stress
Anti-Apoptotic Gene Ced-9
Saline Stress
Estrés Salino
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1045
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1045
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network_name_str INTA Digital (INTA)
spelling Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formationRobert, GermanMuñoz, Nacira BelenMelchiorre, MarianaSanchez, FedericoLascano, Hernan RamiroGlycine MaxSojaBradyrhizobium JaponicumEstrés OsmóticoGenesSoybeansOsmotic StressAnti-Apoptotic Gene Ced-9Saline StressEstrés SalinoThe mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina2017-08-25T16:48:20Z2017-08-25T16:48:20Z2014-07-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1045http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable:Robert G, Muñoz N, Melchiorre M, Sánchez F, Lascano R (2014) Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation. PLoS ONE 9(7): e101747. doi:10.1371/journal.pone.01017471932-6203https://doi.org/10.1371/journal.pone.0101747enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuaria2025-10-16T09:28:59Zoai:localhost:20.500.12123/1045instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:28:59.569INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
title Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
spellingShingle Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
Robert, German
Glycine Max
Soja
Bradyrhizobium Japonicum
Estrés Osmótico
Genes
Soybeans
Osmotic Stress
Anti-Apoptotic Gene Ced-9
Saline Stress
Estrés Salino
title_short Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
title_full Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
title_fullStr Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
title_full_unstemmed Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
title_sort Expression of animal anti-apoptotic gene ced-9 enhances tolerance during Glycine max L.– Bradyrhizobium japonicum interaction under saline stress but reduces nodule formation
dc.creator.none.fl_str_mv Robert, German
Muñoz, Nacira Belen
Melchiorre, Mariana
Sanchez, Federico
Lascano, Hernan Ramiro
author Robert, German
author_facet Robert, German
Muñoz, Nacira Belen
Melchiorre, Mariana
Sanchez, Federico
Lascano, Hernan Ramiro
author_role author
author2 Muñoz, Nacira Belen
Melchiorre, Mariana
Sanchez, Federico
Lascano, Hernan Ramiro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Glycine Max
Soja
Bradyrhizobium Japonicum
Estrés Osmótico
Genes
Soybeans
Osmotic Stress
Anti-Apoptotic Gene Ced-9
Saline Stress
Estrés Salino
topic Glycine Max
Soja
Bradyrhizobium Japonicum
Estrés Osmótico
Genes
Soybeans
Osmotic Stress
Anti-Apoptotic Gene Ced-9
Saline Stress
Estrés Salino
dc.description.none.fl_txt_mv The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
description The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-27
2017-08-25T16:48:20Z
2017-08-25T16:48:20Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1045
http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable
:Robert G, Muñoz N, Melchiorre M, Sánchez F, Lascano R (2014) Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation. PLoS ONE 9(7): e101747. doi:10.1371/journal.pone.0101747
1932-6203
https://doi.org/10.1371/journal.pone.0101747
url http://hdl.handle.net/20.500.12123/1045
http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0101747&type=printable
https://doi.org/10.1371/journal.pone.0101747
identifier_str_mv :Robert G, Muñoz N, Melchiorre M, Sánchez F, Lascano R (2014) Expression of Animal Anti-Apoptotic Gene Ced-9 Enhances Tolerance during Glycine max L.–Bradyrhizobium japonicum Interaction under Saline Stress but Reduces Nodule Formation. PLoS ONE 9(7): e101747. doi:10.1371/journal.pone.0101747
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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