Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
- Autores
- Cholich, Luciana Andrea; Pistán, Maria Elena; Cabrera Benítez, Angelica; Torres, Ana María; Garcia, Enrique Nicolas; Martinez, Agustin; Gardner, Dale R.; Bustillo, Soledad
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 μg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods.
EEA Bariloche
Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Cholich, Luciana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pistán, Maria Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Pistán, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera Benítez, Angelica. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Torres, Ana María. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; Argentina
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; Argentina
Fil: Garcia, Enrique Nicolas. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fil: Gardner, Dale R. US Department of Agriculture. Agricultural Research Service (ARS). Poisonous Plant Research Laboratory; Estados Unidos
Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; Argentina
Fil: Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; Argentina - Fuente
- Toxicon 241 : 107665. (April 2024)
- Materia
-
Prosopis
Toxicidad
Citotoxicidad
Alcaloides
Toxicity
Cytotoxicity
Alkaloids
Apoptosis
Neltuma alpataco
Prosopis alpataco - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/16994
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Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extractCholich, Luciana AndreaPistán, Maria ElenaCabrera Benítez, AngelicaTorres, Ana MaríaGarcia, Enrique NicolasMartinez, AgustinGardner, Dale R.Bustillo, SoledadProsopisToxicidadCitotoxicidadAlcaloidesToxicityCytotoxicityAlkaloidsApoptosisNeltuma alpatacoProsopis alpatacoThe pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 μg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods.EEA BarilocheFil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Cholich, Luciana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pistán, Maria Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Pistán, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabrera Benítez, Angelica. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Torres, Ana María. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; ArgentinaFil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; ArgentinaFil: Garcia, Enrique Nicolas. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; ArgentinaFil: Gardner, Dale R. US Department of Agriculture. Agricultural Research Service (ARS). Poisonous Plant Research Laboratory; Estados UnidosFil: Bustillo, Soledad. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; ArgentinaFil: Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; ArgentinaElsevier2024-03-11T11:43:44Z2024-03-11T11:43:44Z2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16994https://www.sciencedirect.com/science/article/pii/S004101012400237X0041-01011879-3150https://doi.org/10.1016/j.toxicon.2024.107665Toxicon 241 : 107665. (April 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:32Zoai:localhost:20.500.12123/16994instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:32.255INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract |
title |
Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract |
spellingShingle |
Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract Cholich, Luciana Andrea Prosopis Toxicidad Citotoxicidad Alcaloides Toxicity Cytotoxicity Alkaloids Apoptosis Neltuma alpataco Prosopis alpataco |
title_short |
Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract |
title_full |
Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract |
title_fullStr |
Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract |
title_full_unstemmed |
Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract |
title_sort |
Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract |
dc.creator.none.fl_str_mv |
Cholich, Luciana Andrea Pistán, Maria Elena Cabrera Benítez, Angelica Torres, Ana María Garcia, Enrique Nicolas Martinez, Agustin Gardner, Dale R. Bustillo, Soledad |
author |
Cholich, Luciana Andrea |
author_facet |
Cholich, Luciana Andrea Pistán, Maria Elena Cabrera Benítez, Angelica Torres, Ana María Garcia, Enrique Nicolas Martinez, Agustin Gardner, Dale R. Bustillo, Soledad |
author_role |
author |
author2 |
Pistán, Maria Elena Cabrera Benítez, Angelica Torres, Ana María Garcia, Enrique Nicolas Martinez, Agustin Gardner, Dale R. Bustillo, Soledad |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Prosopis Toxicidad Citotoxicidad Alcaloides Toxicity Cytotoxicity Alkaloids Apoptosis Neltuma alpataco Prosopis alpataco |
topic |
Prosopis Toxicidad Citotoxicidad Alcaloides Toxicity Cytotoxicity Alkaloids Apoptosis Neltuma alpataco Prosopis alpataco |
dc.description.none.fl_txt_mv |
The pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 μg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods. EEA Bariloche Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Cholich, Luciana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pistán, Maria Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Pistán, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cabrera Benítez, Angelica. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Torres, Ana María. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; Argentina Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; Argentina Fil: Garcia, Enrique Nicolas. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina Fil: Gardner, Dale R. US Department of Agriculture. Agricultural Research Service (ARS). Poisonous Plant Research Laboratory; Estados Unidos Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; Argentina Fil: Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; Argentina |
description |
The pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 μg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-03-11T11:43:44Z 2024-03-11T11:43:44Z 2024-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/16994 https://www.sciencedirect.com/science/article/pii/S004101012400237X 0041-0101 1879-3150 https://doi.org/10.1016/j.toxicon.2024.107665 |
url |
http://hdl.handle.net/20.500.12123/16994 https://www.sciencedirect.com/science/article/pii/S004101012400237X https://doi.org/10.1016/j.toxicon.2024.107665 |
identifier_str_mv |
0041-0101 1879-3150 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
restrictedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Toxicon 241 : 107665. (April 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1846143569448927232 |
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12.712165 |