Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract

Autores
Cholich, Luciana Andrea; Pistán, Maria Elena; Cabrera Benítez, Angelica; Torres, Ana María; Garcia, Enrique Nicolas; Martinez, Agustin; Gardner, Dale R.; Bustillo, Soledad
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 μg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods.
EEA Bariloche
Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Cholich, Luciana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pistán, Maria Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Pistán, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera Benítez, Angelica. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Torres, Ana María. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; Argentina
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; Argentina
Fil: Garcia, Enrique Nicolas. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fil: Gardner, Dale R. US Department of Agriculture. Agricultural Research Service (ARS). Poisonous Plant Research Laboratory; Estados Unidos
Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; Argentina
Fil: Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; Argentina
Fuente
Toxicon 241 : 107665. (April 2024)
Materia
Prosopis
Toxicidad
Citotoxicidad
Alcaloides
Toxicity
Cytotoxicity
Alkaloids
Apoptosis
Neltuma alpataco
Prosopis alpataco
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extractCholich, Luciana AndreaPistán, Maria ElenaCabrera Benítez, AngelicaTorres, Ana MaríaGarcia, Enrique NicolasMartinez, AgustinGardner, Dale R.Bustillo, SoledadProsopisToxicidadCitotoxicidadAlcaloidesToxicityCytotoxicityAlkaloidsApoptosisNeltuma alpatacoProsopis alpatacoThe pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 μg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods.EEA BarilocheFil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Cholich, Luciana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pistán, Maria Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Pistán, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabrera Benítez, Angelica. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Torres, Ana María. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; ArgentinaFil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; ArgentinaFil: Garcia, Enrique Nicolas. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; ArgentinaFil: Gardner, Dale R. US Department of Agriculture. Agricultural Research Service (ARS). Poisonous Plant Research Laboratory; Estados UnidosFil: Bustillo, Soledad. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; ArgentinaFil: Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; ArgentinaElsevier2024-03-11T11:43:44Z2024-03-11T11:43:44Z2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16994https://www.sciencedirect.com/science/article/pii/S004101012400237X0041-01011879-3150https://doi.org/10.1016/j.toxicon.2024.107665Toxicon 241 : 107665. (April 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:32Zoai:localhost:20.500.12123/16994instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:32.255INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
title Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
spellingShingle Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
Cholich, Luciana Andrea
Prosopis
Toxicidad
Citotoxicidad
Alcaloides
Toxicity
Cytotoxicity
Alkaloids
Apoptosis
Neltuma alpataco
Prosopis alpataco
title_short Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
title_full Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
title_fullStr Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
title_full_unstemmed Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
title_sort Evaluation of the toxicological effects of Neltuma alpataco (Prosopis alpataco) pod alkaloid extract
dc.creator.none.fl_str_mv Cholich, Luciana Andrea
Pistán, Maria Elena
Cabrera Benítez, Angelica
Torres, Ana María
Garcia, Enrique Nicolas
Martinez, Agustin
Gardner, Dale R.
Bustillo, Soledad
author Cholich, Luciana Andrea
author_facet Cholich, Luciana Andrea
Pistán, Maria Elena
Cabrera Benítez, Angelica
Torres, Ana María
Garcia, Enrique Nicolas
Martinez, Agustin
Gardner, Dale R.
Bustillo, Soledad
author_role author
author2 Pistán, Maria Elena
Cabrera Benítez, Angelica
Torres, Ana María
Garcia, Enrique Nicolas
Martinez, Agustin
Gardner, Dale R.
Bustillo, Soledad
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Prosopis
Toxicidad
Citotoxicidad
Alcaloides
Toxicity
Cytotoxicity
Alkaloids
Apoptosis
Neltuma alpataco
Prosopis alpataco
topic Prosopis
Toxicidad
Citotoxicidad
Alcaloides
Toxicity
Cytotoxicity
Alkaloids
Apoptosis
Neltuma alpataco
Prosopis alpataco
dc.description.none.fl_txt_mv The pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 μg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods.
EEA Bariloche
Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Cholich, Luciana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pistán, Maria Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Pistán, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera Benítez, Angelica. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Torres, Ana María. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; Argentina
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Laboratorio de Productos Naturales; Argentina
Fil: Garcia, Enrique Nicolas. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Martinez, Agustin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fil: Gardner, Dale R. US Department of Agriculture. Agricultural Research Service (ARS). Poisonous Plant Research Laboratory; Estados Unidos
Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; Argentina
Fil: Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino. Grupo de Investigaciones Biológicas y Moleculares; Argentina
description The pods of Neltuma spp. have shown potential as a source of protein and energy in livestock. However, prolonged consumption of some of these species can lead to neurological symptoms in ruminants. This study aimed to determine the alkaloid content, as well as the in vitro and in vivo effects of an alkaloid-enriched extract (AEE) from N. alpataco pods. High performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS) identified juliprosine and juliprosopine as primary alkaloids, with juliprosine being most abundant. AEE from N. alpataco demonstrated dose-dependent cytotoxicity on glioma cells after 48 h, with a 50% cytotoxic concentration (CC50) of 24.69 μg/mL. However, the release of LDH was observed only at the highest tested concentration, indicating cellular damage. Further examination through phase-contrast microscopy and dual acridine orange/ethidium bromide fluorescence staining revealed morphological changes consistent with an apoptotic mechanism of cell death, ultimately leading to secondary necrosis. Finally, the LD50 after intraperitoneal injection in mice was determined to be 12.98 mg/kg. Taken together, these findings demonstrated for the first time the in vivo and in vitro toxicity of the AEE from N. alpataco pods.
publishDate 2024
dc.date.none.fl_str_mv 2024-03-11T11:43:44Z
2024-03-11T11:43:44Z
2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16994
https://www.sciencedirect.com/science/article/pii/S004101012400237X
0041-0101
1879-3150
https://doi.org/10.1016/j.toxicon.2024.107665
url http://hdl.handle.net/20.500.12123/16994
https://www.sciencedirect.com/science/article/pii/S004101012400237X
https://doi.org/10.1016/j.toxicon.2024.107665
identifier_str_mv 0041-0101
1879-3150
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Toxicon 241 : 107665. (April 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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