Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers

Autores
Perez De La Torre, Mariana; Escandon, Alejandro Salvio; Heinz, Ruth Amelia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Calibrachoa caesia is one of thirteen native species of the genus Calibrachoa Cerv. ex La Lave et Lexarza (Solanaceae) growing in Argentina. The genus has a high ornamental value due to the morphological variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from populations of three departments in the Province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by Inter simple sequence repeat (ISSR) markers. Thirteen ISSR primers produced reproducible bands, with 652 amplified and 97.4% polymorphism. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá populations, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The analysis of molecular variance (AMOVA) test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations, showing a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programs
Instituto de Floricultura
Fil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Escandon, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Acta Horticulturae 1000 : 1-8 (2013)
Materia
Organismos Indígenas
Solanaceae
Plantas Ornamentales
Variación Genética
Marcadores Genéticos
Indigenous Organisms
Ornamental Plants
Genetic Variation
Genetic Markers
Calibrachoa caesia
Especies Nativas
Diversidad Genética
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3891
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3891
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network_name_str INTA Digital (INTA)
spelling Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markersPerez De La Torre, MarianaEscandon, Alejandro SalvioHeinz, Ruth AmeliaOrganismos IndígenasSolanaceaePlantas OrnamentalesVariación GenéticaMarcadores GenéticosIndigenous OrganismsOrnamental PlantsGenetic VariationGenetic MarkersCalibrachoa caesiaEspecies NativasDiversidad GenéticaArgentinaCalibrachoa caesia is one of thirteen native species of the genus Calibrachoa Cerv. ex La Lave et Lexarza (Solanaceae) growing in Argentina. The genus has a high ornamental value due to the morphological variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from populations of three departments in the Province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by Inter simple sequence repeat (ISSR) markers. Thirteen ISSR primers produced reproducible bands, with 652 amplified and 97.4% polymorphism. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá populations, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The analysis of molecular variance (AMOVA) test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations, showing a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programsInstituto de FloriculturaFil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; ArgentinaFil: Escandon, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaISHS2018-11-14T14:44:15Z2018-11-14T14:44:15Z2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.actahort.org/books/1000/1000_61.htmhttp://hdl.handle.net/20.500.12123/3891978-90-66055-76-60567-75722406-6168http://dx.doi.org/10.17660/ActaHortic.2013.1000.61Acta Horticulturae 1000 : 1-8 (2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:44Zoai:localhost:20.500.12123/3891instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:44.716INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
spellingShingle Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
Perez De La Torre, Mariana
Organismos Indígenas
Solanaceae
Plantas Ornamentales
Variación Genética
Marcadores Genéticos
Indigenous Organisms
Ornamental Plants
Genetic Variation
Genetic Markers
Calibrachoa caesia
Especies Nativas
Diversidad Genética
Argentina
title_short Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title_full Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title_fullStr Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title_full_unstemmed Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title_sort Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
dc.creator.none.fl_str_mv Perez De La Torre, Mariana
Escandon, Alejandro Salvio
Heinz, Ruth Amelia
author Perez De La Torre, Mariana
author_facet Perez De La Torre, Mariana
Escandon, Alejandro Salvio
Heinz, Ruth Amelia
author_role author
author2 Escandon, Alejandro Salvio
Heinz, Ruth Amelia
author2_role author
author
dc.subject.none.fl_str_mv Organismos Indígenas
Solanaceae
Plantas Ornamentales
Variación Genética
Marcadores Genéticos
Indigenous Organisms
Ornamental Plants
Genetic Variation
Genetic Markers
Calibrachoa caesia
Especies Nativas
Diversidad Genética
Argentina
topic Organismos Indígenas
Solanaceae
Plantas Ornamentales
Variación Genética
Marcadores Genéticos
Indigenous Organisms
Ornamental Plants
Genetic Variation
Genetic Markers
Calibrachoa caesia
Especies Nativas
Diversidad Genética
Argentina
dc.description.none.fl_txt_mv Calibrachoa caesia is one of thirteen native species of the genus Calibrachoa Cerv. ex La Lave et Lexarza (Solanaceae) growing in Argentina. The genus has a high ornamental value due to the morphological variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from populations of three departments in the Province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by Inter simple sequence repeat (ISSR) markers. Thirteen ISSR primers produced reproducible bands, with 652 amplified and 97.4% polymorphism. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá populations, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The analysis of molecular variance (AMOVA) test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations, showing a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programs
Instituto de Floricultura
Fil: Perez De La Torre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina
Fil: Escandon, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Calibrachoa caesia is one of thirteen native species of the genus Calibrachoa Cerv. ex La Lave et Lexarza (Solanaceae) growing in Argentina. The genus has a high ornamental value due to the morphological variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from populations of three departments in the Province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by Inter simple sequence repeat (ISSR) markers. Thirteen ISSR primers produced reproducible bands, with 652 amplified and 97.4% polymorphism. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá populations, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The analysis of molecular variance (AMOVA) test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations, showing a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programs
publishDate 2013
dc.date.none.fl_str_mv 2013-07
2018-11-14T14:44:15Z
2018-11-14T14:44:15Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.actahort.org/books/1000/1000_61.htm
http://hdl.handle.net/20.500.12123/3891
978-90-66055-76-6
0567-7572
2406-6168
http://dx.doi.org/10.17660/ActaHortic.2013.1000.61
url https://www.actahort.org/books/1000/1000_61.htm
http://hdl.handle.net/20.500.12123/3891
http://dx.doi.org/10.17660/ActaHortic.2013.1000.61
identifier_str_mv 978-90-66055-76-6
0567-7572
2406-6168
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISHS
publisher.none.fl_str_mv ISHS
dc.source.none.fl_str_mv Acta Horticulturae 1000 : 1-8 (2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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