Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers

Autores
Perez de la Torre, Mariana Cecilia; Heinz, Ruth Amelia; Escandón, Alejandro Salvio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Calibrachoa caesia is one of thirteen native species of the Calibrachoa Cerv. (ex La Lave & Lexarza) genus, Solanaceae family,growing in Argentina. The genus has a high ornamental value due to the variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from three departments in the province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by ISSR markers. Thirteen ISSR primers produced a reproducible banding pattern, with 652 amplified loci and 97.4% polymorphism value. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá departments, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The AMOVA test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programs.
Fil: Perez de la Torre, Mariana Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Anchored
Microsatellites
Molecular
Markers
Ornamental
Plants
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26591
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markersPerez de la Torre, Mariana CeciliaHeinz, Ruth AmeliaEscandón, Alejandro SalvioAnchoredMicrosatellitesMolecularMarkersOrnamentalPlantshttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Calibrachoa caesia is one of thirteen native species of the Calibrachoa Cerv. (ex La Lave & Lexarza) genus, Solanaceae family,growing in Argentina. The genus has a high ornamental value due to the variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from three departments in the province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by ISSR markers. Thirteen ISSR primers produced a reproducible banding pattern, with 652 amplified loci and 97.4% polymorphism value. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá departments, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The AMOVA test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programs.Fil: Perez de la Torre, Mariana Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInternational Society for Horticultural Science2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26591Perez de la Torre, Mariana Cecilia; Heinz, Ruth Amelia; Escandón, Alejandro Salvio; Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers; International Society for Horticultural Science; Acta Horticulturae; 100; 9-2013; 439-4440567-7572CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.actahort.org/books/1000/1000_61.htminfo:eu-repo/semantics/altIdentifier/doi/10.17660/ActaHortic.2013.1000.61info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:18Zoai:ri.conicet.gov.ar:11336/26591instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:18.467CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
spellingShingle Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
Perez de la Torre, Mariana Cecilia
Anchored
Microsatellites
Molecular
Markers
Ornamental
Plants
title_short Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title_full Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title_fullStr Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title_full_unstemmed Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
title_sort Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers
dc.creator.none.fl_str_mv Perez de la Torre, Mariana Cecilia
Heinz, Ruth Amelia
Escandón, Alejandro Salvio
author Perez de la Torre, Mariana Cecilia
author_facet Perez de la Torre, Mariana Cecilia
Heinz, Ruth Amelia
Escandón, Alejandro Salvio
author_role author
author2 Heinz, Ruth Amelia
Escandón, Alejandro Salvio
author2_role author
author
dc.subject.none.fl_str_mv Anchored
Microsatellites
Molecular
Markers
Ornamental
Plants
topic Anchored
Microsatellites
Molecular
Markers
Ornamental
Plants
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Calibrachoa caesia is one of thirteen native species of the Calibrachoa Cerv. (ex La Lave & Lexarza) genus, Solanaceae family,growing in Argentina. The genus has a high ornamental value due to the variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from three departments in the province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by ISSR markers. Thirteen ISSR primers produced a reproducible banding pattern, with 652 amplified loci and 97.4% polymorphism value. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá departments, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The AMOVA test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programs.
Fil: Perez de la Torre, Mariana Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Calibrachoa caesia is one of thirteen native species of the Calibrachoa Cerv. (ex La Lave & Lexarza) genus, Solanaceae family,growing in Argentina. The genus has a high ornamental value due to the variability that it shows for different genetic characters, including the flower colors. The structure and variation of 29 accessions from three departments in the province of Misiones (Oberá, Guaraní and San Ignacio) were analyzed by ISSR markers. Thirteen ISSR primers produced a reproducible banding pattern, with 652 amplified loci and 97.4% polymorphism value. The polymorphism information content ranged from 0.144 to 0.170 for accessions from San Ignacio and Oberá departments, being the average 0.158, whereas the Shannon Index showed an average of 0.256. The principal coordinate analysis showed that the San Ignacio individuals were more disperse than the Guaraní and Oberá accessions. The AMOVA test for Guaraní, Oberá and San Ignacio populations, showed highest genetic variation within populations (93.59%), meanwhile the Fst coefficient was 0.064, indicating a low to medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among San Ignacio, Oberá and Guaraní populations. According to these results, the highly polymorphic level of the 29 analyzed individuals from the three locations represents an important source of genetic variability for future breeding programs.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26591
Perez de la Torre, Mariana Cecilia; Heinz, Ruth Amelia; Escandón, Alejandro Salvio; Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers; International Society for Horticultural Science; Acta Horticulturae; 100; 9-2013; 439-444
0567-7572
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26591
identifier_str_mv Perez de la Torre, Mariana Cecilia; Heinz, Ruth Amelia; Escandón, Alejandro Salvio; Structure and genetic diversity of three Calibrachoa caesia populations by ISSR markers; International Society for Horticultural Science; Acta Horticulturae; 100; 9-2013; 439-444
0567-7572
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.actahort.org/books/1000/1000_61.htm
info:eu-repo/semantics/altIdentifier/doi/10.17660/ActaHortic.2013.1000.61
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Society for Horticultural Science
publisher.none.fl_str_mv International Society for Horticultural Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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