Analysis of genetic variability by ISSR markers in Calibrachoa caesia

Autores
Escandón, Alejandro Salvio; Garcia, Martín Nahuel; Heinz, Ruth Amelia; Escandón, Alejandro Salvio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5?CT, 5?CA, 5?GA, 5?GACA, 3?CAC, 3?TG and 3?TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5?GACA and 3?AG primers, respectively, while the averagevalues for MI and PIC were 0.367 and 0.231, respectively. The more informative primers were 5?GACA and 5?GA, and the less informative was 3?AC. Simple matching coefficient of similarity varied from 0.8875 to 0.6659, indicating high levels of genetic similarity among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters; one comprised genotypes of the five departments, while the second included individuals from Guaraní and Oberá regions and the third cluster included the San Pedro individuals. The overall grouping pattern is in agreement with principal coordinate analysis (PCoA). Conclusions: The Bayesian cluster analysis revealed structuring of the C. caesia population and two clusters were identified, which correspond to UPGMA major clades. The AMOVA test for all populations showed highest genetic variation within populations (90%), meanwhile the Fst coefficient was 0.098, indicating a medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among populations. In this work the use of thirteen ISSR markers, allowed the characterization of every individual examined. Therefore, the possibility of using molecular data for varietal identification is a promising tool for application in future breeding programs in the genus Calibrachoa.
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Garcia, Martín Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Materia
ANCHORED MICROSATELLITES
MOLECULAR MARKERS
ORNAMENTAL PLANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243462
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/243462
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of genetic variability by ISSR markers in Calibrachoa caesiaEscandón, Alejandro SalvioGarcia, Martín NahuelHeinz, Ruth AmeliaEscandón, Alejandro SalvioANCHORED MICROSATELLITESMOLECULAR MARKERSORNAMENTAL PLANTShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5?CT, 5?CA, 5?GA, 5?GACA, 3?CAC, 3?TG and 3?TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5?GACA and 3?AG primers, respectively, while the averagevalues for MI and PIC were 0.367 and 0.231, respectively. The more informative primers were 5?GACA and 5?GA, and the less informative was 3?AC. Simple matching coefficient of similarity varied from 0.8875 to 0.6659, indicating high levels of genetic similarity among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters; one comprised genotypes of the five departments, while the second included individuals from Guaraní and Oberá regions and the third cluster included the San Pedro individuals. The overall grouping pattern is in agreement with principal coordinate analysis (PCoA). Conclusions: The Bayesian cluster analysis revealed structuring of the C. caesia population and two clusters were identified, which correspond to UPGMA major clades. The AMOVA test for all populations showed highest genetic variation within populations (90%), meanwhile the Fst coefficient was 0.098, indicating a medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among populations. In this work the use of thirteen ISSR markers, allowed the characterization of every individual examined. Therefore, the possibility of using molecular data for varietal identification is a promising tool for application in future breeding programs in the genus Calibrachoa.Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; ArgentinaFil: Garcia, Martín Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaPontificia Universidad Católica de Valparaíso2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243462Escandón, Alejandro Salvio; Garcia, Martín Nahuel; Heinz, Ruth Amelia; Escandón, Alejandro Salvio; Analysis of genetic variability by ISSR markers in Calibrachoa caesia; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 15; 5; 9-2012; 8-80717-3458CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.cl/pdf/ejb/v15n5/a08.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.2225/vol15-issue5-fulltext-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:41Zoai:ri.conicet.gov.ar:11336/243462instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:41.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title Analysis of genetic variability by ISSR markers in Calibrachoa caesia
spellingShingle Analysis of genetic variability by ISSR markers in Calibrachoa caesia
Escandón, Alejandro Salvio
ANCHORED MICROSATELLITES
MOLECULAR MARKERS
ORNAMENTAL PLANTS
title_short Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_full Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_fullStr Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_full_unstemmed Analysis of genetic variability by ISSR markers in Calibrachoa caesia
title_sort Analysis of genetic variability by ISSR markers in Calibrachoa caesia
dc.creator.none.fl_str_mv Escandón, Alejandro Salvio
Garcia, Martín Nahuel
Heinz, Ruth Amelia
Escandón, Alejandro Salvio
author Escandón, Alejandro Salvio
author_facet Escandón, Alejandro Salvio
Garcia, Martín Nahuel
Heinz, Ruth Amelia
author_role author
author2 Garcia, Martín Nahuel
Heinz, Ruth Amelia
author2_role author
author
dc.subject.none.fl_str_mv ANCHORED MICROSATELLITES
MOLECULAR MARKERS
ORNAMENTAL PLANTS
topic ANCHORED MICROSATELLITES
MOLECULAR MARKERS
ORNAMENTAL PLANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5?CT, 5?CA, 5?GA, 5?GACA, 3?CAC, 3?TG and 3?TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5?GACA and 3?AG primers, respectively, while the averagevalues for MI and PIC were 0.367 and 0.231, respectively. The more informative primers were 5?GACA and 5?GA, and the less informative was 3?AC. Simple matching coefficient of similarity varied from 0.8875 to 0.6659, indicating high levels of genetic similarity among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters; one comprised genotypes of the five departments, while the second included individuals from Guaraní and Oberá regions and the third cluster included the San Pedro individuals. The overall grouping pattern is in agreement with principal coordinate analysis (PCoA). Conclusions: The Bayesian cluster analysis revealed structuring of the C. caesia population and two clusters were identified, which correspond to UPGMA major clades. The AMOVA test for all populations showed highest genetic variation within populations (90%), meanwhile the Fst coefficient was 0.098, indicating a medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among populations. In this work the use of thirteen ISSR markers, allowed the characterization of every individual examined. Therefore, the possibility of using molecular data for varietal identification is a promising tool for application in future breeding programs in the genus Calibrachoa.
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Garcia, Martín Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
description Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5?CT, 5?CA, 5?GA, 5?GACA, 3?CAC, 3?TG and 3?TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5?GACA and 3?AG primers, respectively, while the averagevalues for MI and PIC were 0.367 and 0.231, respectively. The more informative primers were 5?GACA and 5?GA, and the less informative was 3?AC. Simple matching coefficient of similarity varied from 0.8875 to 0.6659, indicating high levels of genetic similarity among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters; one comprised genotypes of the five departments, while the second included individuals from Guaraní and Oberá regions and the third cluster included the San Pedro individuals. The overall grouping pattern is in agreement with principal coordinate analysis (PCoA). Conclusions: The Bayesian cluster analysis revealed structuring of the C. caesia population and two clusters were identified, which correspond to UPGMA major clades. The AMOVA test for all populations showed highest genetic variation within populations (90%), meanwhile the Fst coefficient was 0.098, indicating a medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among populations. In this work the use of thirteen ISSR markers, allowed the characterization of every individual examined. Therefore, the possibility of using molecular data for varietal identification is a promising tool for application in future breeding programs in the genus Calibrachoa.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243462
Escandón, Alejandro Salvio; Garcia, Martín Nahuel; Heinz, Ruth Amelia; Escandón, Alejandro Salvio; Analysis of genetic variability by ISSR markers in Calibrachoa caesia; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 15; 5; 9-2012; 8-8
0717-3458
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243462
identifier_str_mv Escandón, Alejandro Salvio; Garcia, Martín Nahuel; Heinz, Ruth Amelia; Escandón, Alejandro Salvio; Analysis of genetic variability by ISSR markers in Calibrachoa caesia; Pontificia Universidad Católica de Valparaíso; Electronic Journal of Biotechnology; 15; 5; 9-2012; 8-8
0717-3458
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scielo.cl/pdf/ejb/v15n5/a08.pdf
info:eu-repo/semantics/altIdentifier/doi/10.2225/vol15-issue5-fulltext-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
publisher.none.fl_str_mv Pontificia Universidad Católica de Valparaíso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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