Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination

Autores
Gomes, Karina A.; Rivero, Karina A.; Barrios Mathieur, Christian; Degiuseppe, Juan I.; Cortes, Paulo R.; Gonzalez, Patricia A.; Zurschmitten, Abel; Castro, María P.; Parreño, Gladys Viviana; Mozgovoj, Marina Valeria; Stupka, Juan A.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Norovirus (NoV) is a leading cause of acute gastroenteritis (AGE) in young children worldwide. Following the introduction of universal rotavirus (RVA) vaccination in Argentina in 2015, the role of NoV in pediatric AGE warrants evaluation. This study aimed to assess the prevalence, clinical characteristics, and molecular diversity of NoV in children under five years of age, comparing the periods before and after RVA vaccine implementation. Methods: A descriptive observational study was conducted in two pediatric hospitals in Argentina. Stool samples were obtained from both outpatient and hospitalized children presenting with acute gastroenteritis (AGE) during two distinct one-year periods: 285 samples from the pre-vaccination period (2011–2012) and 212 samples from the post-vaccination period (2019–2020). NoV, RVA and other viral enteropathogens were detected by RT-qPCR or immunoassay. Positive NoV samples were genotyped by Sanger sequencing of the ORF1/ORF2 junction. Results: NoV was detected in 30.1% (86/285) and 23.5% (50/212) of cases in the pre- and post-vaccination periods, respectively. Children under two years of age and inpatients had significantly higher NoV detection in both periods. NoV mono-infections were more frequent in post-vaccination period (72% vs. 50%). NoV GII predominated in both periods, with increased genotype diversity observed post-vaccination, including GII.3[P12], GII.4 Sydney[P16], GII.6[P7], and GII.2[P16]. Conclusions: NoV remains a major cause of pediatric AGE in Argentina, particularly in children under two years old. Although NoV prevalence did not increase after RVA vaccine introduction, its clinical relevance persists. Continued molecular surveillance is essential to monitor genotype dynamics and implement prevention strategies.
Instituto de Virología
Fil: Gomes, Karina A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina
Fil: Rivero, Karina A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina
Fil: Barrios Mathieur, Christian. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina
Fil: Degiuseppe, Juan I. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina
Fil: Cortes, Paulo R. Hospital de Niños “Del Niño Jesús” (Córdoba). Microbiology Laboratory; Argentina
Fil: Gonzalez, Patricia A. Hospital de Niños “Del Niño Jesús” (Córdoba). Microbiology Laboratory; Argentina
Fil: Zurschmitten, Abel. Hospital “Junín de los Andes” Hospital. Laboratory Section; Argentina
Fil: Castro, María P. Hospital “Junín de los Andes” Hospital. Laboratory Section; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina.
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.
Fil: Mozgovoj, Marina Valeria. Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS) UEDD INTA-CONICET; Argentina.
Fuente
Vaccines 13 (11) : 1080. (November 2025)
Materia
Norovirus
Viruses
Children
Gastroenteritis
Vaccination
Virus
Niños
Vacunación
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24775
Acceder
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oai_identifier_str oai:localhost:20.500.12123/24775
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus VaccinationGomes, Karina A.Rivero, Karina A.Barrios Mathieur, ChristianDegiuseppe, Juan I.Cortes, Paulo R.Gonzalez, Patricia A.Zurschmitten, AbelCastro, María P.Parreño, Gladys VivianaMozgovoj, Marina ValeriaStupka, Juan A.NorovirusVirusesChildrenGastroenteritisVaccinationVirusNiñosVacunaciónArgentinaNorovirus (NoV) is a leading cause of acute gastroenteritis (AGE) in young children worldwide. Following the introduction of universal rotavirus (RVA) vaccination in Argentina in 2015, the role of NoV in pediatric AGE warrants evaluation. This study aimed to assess the prevalence, clinical characteristics, and molecular diversity of NoV in children under five years of age, comparing the periods before and after RVA vaccine implementation. Methods: A descriptive observational study was conducted in two pediatric hospitals in Argentina. Stool samples were obtained from both outpatient and hospitalized children presenting with acute gastroenteritis (AGE) during two distinct one-year periods: 285 samples from the pre-vaccination period (2011–2012) and 212 samples from the post-vaccination period (2019–2020). NoV, RVA and other viral enteropathogens were detected by RT-qPCR or immunoassay. Positive NoV samples were genotyped by Sanger sequencing of the ORF1/ORF2 junction. Results: NoV was detected in 30.1% (86/285) and 23.5% (50/212) of cases in the pre- and post-vaccination periods, respectively. Children under two years of age and inpatients had significantly higher NoV detection in both periods. NoV mono-infections were more frequent in post-vaccination period (72% vs. 50%). NoV GII predominated in both periods, with increased genotype diversity observed post-vaccination, including GII.3[P12], GII.4 Sydney[P16], GII.6[P7], and GII.2[P16]. Conclusions: NoV remains a major cause of pediatric AGE in Argentina, particularly in children under two years old. Although NoV prevalence did not increase after RVA vaccine introduction, its clinical relevance persists. Continued molecular surveillance is essential to monitor genotype dynamics and implement prevention strategies.Instituto de VirologíaFil: Gomes, Karina A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; ArgentinaFil: Rivero, Karina A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; ArgentinaFil: Barrios Mathieur, Christian. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; ArgentinaFil: Degiuseppe, Juan I. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; ArgentinaFil: Cortes, Paulo R. Hospital de Niños “Del Niño Jesús” (Córdoba). Microbiology Laboratory; ArgentinaFil: Gonzalez, Patricia A. Hospital de Niños “Del Niño Jesús” (Córdoba). Microbiology Laboratory; ArgentinaFil: Zurschmitten, Abel. Hospital “Junín de los Andes” Hospital. Laboratory Section; ArgentinaFil: Castro, María P. Hospital “Junín de los Andes” Hospital. Laboratory Section; ArgentinaFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina.Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.Fil: Mozgovoj, Marina Valeria. Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS) UEDD INTA-CONICET; Argentina.MDPI2025-12-26T16:48:23Z2025-12-26T16:48:23Z2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24775https://www.mdpi.com/2076-393X/13/11/10802076-393Xhttps://doi.org/10.3390/vaccines13111080Vaccines 13 (11) : 1080. (November 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-01-08T10:41:01Zoai:localhost:20.500.12123/24775instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:41:01.545INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
title Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
spellingShingle Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
Gomes, Karina A.
Norovirus
Viruses
Children
Gastroenteritis
Vaccination
Virus
Niños
Vacunación
Argentina
title_short Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
title_full Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
title_fullStr Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
title_full_unstemmed Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
title_sort Norovirus in Pediatric Gastroenteritis: A Study in Argentine Hospitals Before and After the Introduction of Universal Rotavirus Vaccination
dc.creator.none.fl_str_mv Gomes, Karina A.
Rivero, Karina A.
Barrios Mathieur, Christian
Degiuseppe, Juan I.
Cortes, Paulo R.
Gonzalez, Patricia A.
Zurschmitten, Abel
Castro, María P.
Parreño, Gladys Viviana
Mozgovoj, Marina Valeria
Stupka, Juan A.
author Gomes, Karina A.
author_facet Gomes, Karina A.
Rivero, Karina A.
Barrios Mathieur, Christian
Degiuseppe, Juan I.
Cortes, Paulo R.
Gonzalez, Patricia A.
Zurschmitten, Abel
Castro, María P.
Parreño, Gladys Viviana
Mozgovoj, Marina Valeria
Stupka, Juan A.
author_role author
author2 Rivero, Karina A.
Barrios Mathieur, Christian
Degiuseppe, Juan I.
Cortes, Paulo R.
Gonzalez, Patricia A.
Zurschmitten, Abel
Castro, María P.
Parreño, Gladys Viviana
Mozgovoj, Marina Valeria
Stupka, Juan A.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Norovirus
Viruses
Children
Gastroenteritis
Vaccination
Virus
Niños
Vacunación
Argentina
topic Norovirus
Viruses
Children
Gastroenteritis
Vaccination
Virus
Niños
Vacunación
Argentina
dc.description.none.fl_txt_mv Norovirus (NoV) is a leading cause of acute gastroenteritis (AGE) in young children worldwide. Following the introduction of universal rotavirus (RVA) vaccination in Argentina in 2015, the role of NoV in pediatric AGE warrants evaluation. This study aimed to assess the prevalence, clinical characteristics, and molecular diversity of NoV in children under five years of age, comparing the periods before and after RVA vaccine implementation. Methods: A descriptive observational study was conducted in two pediatric hospitals in Argentina. Stool samples were obtained from both outpatient and hospitalized children presenting with acute gastroenteritis (AGE) during two distinct one-year periods: 285 samples from the pre-vaccination period (2011–2012) and 212 samples from the post-vaccination period (2019–2020). NoV, RVA and other viral enteropathogens were detected by RT-qPCR or immunoassay. Positive NoV samples were genotyped by Sanger sequencing of the ORF1/ORF2 junction. Results: NoV was detected in 30.1% (86/285) and 23.5% (50/212) of cases in the pre- and post-vaccination periods, respectively. Children under two years of age and inpatients had significantly higher NoV detection in both periods. NoV mono-infections were more frequent in post-vaccination period (72% vs. 50%). NoV GII predominated in both periods, with increased genotype diversity observed post-vaccination, including GII.3[P12], GII.4 Sydney[P16], GII.6[P7], and GII.2[P16]. Conclusions: NoV remains a major cause of pediatric AGE in Argentina, particularly in children under two years old. Although NoV prevalence did not increase after RVA vaccine introduction, its clinical relevance persists. Continued molecular surveillance is essential to monitor genotype dynamics and implement prevention strategies.
Instituto de Virología
Fil: Gomes, Karina A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina
Fil: Rivero, Karina A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina
Fil: Barrios Mathieur, Christian. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina
Fil: Degiuseppe, Juan I. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Gastroenteritis Viral; Argentina
Fil: Cortes, Paulo R. Hospital de Niños “Del Niño Jesús” (Córdoba). Microbiology Laboratory; Argentina
Fil: Gonzalez, Patricia A. Hospital de Niños “Del Niño Jesús” (Córdoba). Microbiology Laboratory; Argentina
Fil: Zurschmitten, Abel. Hospital “Junín de los Andes” Hospital. Laboratory Section; Argentina
Fil: Castro, María P. Hospital “Junín de los Andes” Hospital. Laboratory Section; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina.
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.
Fil: Mozgovoj, Marina Valeria. Instituto de Ciencia y Tecnología de los Sistemas Alimentarios Sustentables (ICyTeSAS) UEDD INTA-CONICET; Argentina.
description Norovirus (NoV) is a leading cause of acute gastroenteritis (AGE) in young children worldwide. Following the introduction of universal rotavirus (RVA) vaccination in Argentina in 2015, the role of NoV in pediatric AGE warrants evaluation. This study aimed to assess the prevalence, clinical characteristics, and molecular diversity of NoV in children under five years of age, comparing the periods before and after RVA vaccine implementation. Methods: A descriptive observational study was conducted in two pediatric hospitals in Argentina. Stool samples were obtained from both outpatient and hospitalized children presenting with acute gastroenteritis (AGE) during two distinct one-year periods: 285 samples from the pre-vaccination period (2011–2012) and 212 samples from the post-vaccination period (2019–2020). NoV, RVA and other viral enteropathogens were detected by RT-qPCR or immunoassay. Positive NoV samples were genotyped by Sanger sequencing of the ORF1/ORF2 junction. Results: NoV was detected in 30.1% (86/285) and 23.5% (50/212) of cases in the pre- and post-vaccination periods, respectively. Children under two years of age and inpatients had significantly higher NoV detection in both periods. NoV mono-infections were more frequent in post-vaccination period (72% vs. 50%). NoV GII predominated in both periods, with increased genotype diversity observed post-vaccination, including GII.3[P12], GII.4 Sydney[P16], GII.6[P7], and GII.2[P16]. Conclusions: NoV remains a major cause of pediatric AGE in Argentina, particularly in children under two years old. Although NoV prevalence did not increase after RVA vaccine introduction, its clinical relevance persists. Continued molecular surveillance is essential to monitor genotype dynamics and implement prevention strategies.
publishDate 2025
dc.date.none.fl_str_mv 2025-12-26T16:48:23Z
2025-12-26T16:48:23Z
2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24775
https://www.mdpi.com/2076-393X/13/11/1080
2076-393X
https://doi.org/10.3390/vaccines13111080
url http://hdl.handle.net/20.500.12123/24775
https://www.mdpi.com/2076-393X/13/11/1080
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dc.language.none.fl_str_mv eng
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Vaccines 13 (11) : 1080. (November 2025)
reponame:INTA Digital (INTA)
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reponame_str INTA Digital (INTA)
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