Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1

Autores
Zacarias, Maria Florencia; Cuatrin, Alejandra; Lavari, Luisina; Taverna, Miguel Angel; Paez, Roxana Beatriz; Vinderola, Celso Gabriel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Whey permeate (WP), a by-product of cheese whey treatment, constitutes a sustainable and economical alternative to synthetic media for probiotic biomass production. Likewise, spray drying (SD) is a rapid and cost-effective alternative to freeze drying in industrial practice. This study aimed to optimise the production of a spray-dried culture (SDC) of Bifidobacterium animalis subsp. lactis INL1. Biomass production on WP was optimised by analyzing the effects of yeast extract and pH on bacterial growth, survival during spray drying, and resistance to simulated gastric digestion. Additionally, pH, inlet temperature, and drying matrix effects were evaluated for their impact on strain resistance during SD and storage. WP proved to be a suitable ingredient for B. lactis INL1 biomass production. Acidic culture conditions yielded superior results, and all tested factors significantly influenced SDC performance. These findings highlight the usefulness of optimization strategies to define effective production conditions for probiotic cultures.
EEA Rafaela
Fil: Zacarías, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Zacarías, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuatrin, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Lavari, Luisina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taverna, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
International Dairy Journal 163 : 106181. (April 2025)
Materia
Biomasa
Lactosuero
Probióticos
Secado por Pulverización
Biomass
Whey
Bifidobacterium
Probiotics
Spray Drying
Bifidobacterium animalis subsp. lactis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21956
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21956
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1Zacarias, Maria FlorenciaCuatrin, AlejandraLavari, LuisinaTaverna, Miguel AngelPaez, Roxana BeatrizVinderola, Celso GabrielBiomasaLactosueroProbióticosSecado por PulverizaciónBiomassWheyBifidobacteriumProbioticsSpray DryingBifidobacterium animalis subsp. lactisWhey permeate (WP), a by-product of cheese whey treatment, constitutes a sustainable and economical alternative to synthetic media for probiotic biomass production. Likewise, spray drying (SD) is a rapid and cost-effective alternative to freeze drying in industrial practice. This study aimed to optimise the production of a spray-dried culture (SDC) of Bifidobacterium animalis subsp. lactis INL1. Biomass production on WP was optimised by analyzing the effects of yeast extract and pH on bacterial growth, survival during spray drying, and resistance to simulated gastric digestion. Additionally, pH, inlet temperature, and drying matrix effects were evaluated for their impact on strain resistance during SD and storage. WP proved to be a suitable ingredient for B. lactis INL1 biomass production. Acidic culture conditions yielded superior results, and all tested factors significantly influenced SDC performance. These findings highlight the usefulness of optimization strategies to define effective production conditions for probiotic cultures.EEA RafaelaFil: Zacarías, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Zacarías, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cuatrin, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Lavari, Luisina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Taverna, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactología Industrial; ArgentinaFil: Vinderola, Celso Gabriel. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2025-04-08T13:40:02Z2025-04-08T13:40:02Z2025-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21956https://www.sciencedirect.com/science/article/abs/pii/S09586946240030170958-69461879-0143https://doi.org/10.1016/j.idairyj.2024.106181International Dairy Journal 163 : 106181. (April 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNAIyAV-1130032/AR./Tecnologías de transformación de alimentos.info:eu-repograntAgreement/INTA/PNAIyAV-1130033/AR./Tecnologías de preservación de alimentos y de aprovechamiento de subproductos.info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:01Zoai:localhost:20.500.12123/21956instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:01.38INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
spellingShingle Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
Zacarias, Maria Florencia
Biomasa
Lactosuero
Probióticos
Secado por Pulverización
Biomass
Whey
Bifidobacterium
Probiotics
Spray Drying
Bifidobacterium animalis subsp. lactis
title_short Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_full Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_fullStr Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_full_unstemmed Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_sort Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
dc.creator.none.fl_str_mv Zacarias, Maria Florencia
Cuatrin, Alejandra
Lavari, Luisina
Taverna, Miguel Angel
Paez, Roxana Beatriz
Vinderola, Celso Gabriel
author Zacarias, Maria Florencia
author_facet Zacarias, Maria Florencia
Cuatrin, Alejandra
Lavari, Luisina
Taverna, Miguel Angel
Paez, Roxana Beatriz
Vinderola, Celso Gabriel
author_role author
author2 Cuatrin, Alejandra
Lavari, Luisina
Taverna, Miguel Angel
Paez, Roxana Beatriz
Vinderola, Celso Gabriel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biomasa
Lactosuero
Probióticos
Secado por Pulverización
Biomass
Whey
Bifidobacterium
Probiotics
Spray Drying
Bifidobacterium animalis subsp. lactis
topic Biomasa
Lactosuero
Probióticos
Secado por Pulverización
Biomass
Whey
Bifidobacterium
Probiotics
Spray Drying
Bifidobacterium animalis subsp. lactis
dc.description.none.fl_txt_mv Whey permeate (WP), a by-product of cheese whey treatment, constitutes a sustainable and economical alternative to synthetic media for probiotic biomass production. Likewise, spray drying (SD) is a rapid and cost-effective alternative to freeze drying in industrial practice. This study aimed to optimise the production of a spray-dried culture (SDC) of Bifidobacterium animalis subsp. lactis INL1. Biomass production on WP was optimised by analyzing the effects of yeast extract and pH on bacterial growth, survival during spray drying, and resistance to simulated gastric digestion. Additionally, pH, inlet temperature, and drying matrix effects were evaluated for their impact on strain resistance during SD and storage. WP proved to be a suitable ingredient for B. lactis INL1 biomass production. Acidic culture conditions yielded superior results, and all tested factors significantly influenced SDC performance. These findings highlight the usefulness of optimization strategies to define effective production conditions for probiotic cultures.
EEA Rafaela
Fil: Zacarías, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Zacarías, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuatrin, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Lavari, Luisina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taverna, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Whey permeate (WP), a by-product of cheese whey treatment, constitutes a sustainable and economical alternative to synthetic media for probiotic biomass production. Likewise, spray drying (SD) is a rapid and cost-effective alternative to freeze drying in industrial practice. This study aimed to optimise the production of a spray-dried culture (SDC) of Bifidobacterium animalis subsp. lactis INL1. Biomass production on WP was optimised by analyzing the effects of yeast extract and pH on bacterial growth, survival during spray drying, and resistance to simulated gastric digestion. Additionally, pH, inlet temperature, and drying matrix effects were evaluated for their impact on strain resistance during SD and storage. WP proved to be a suitable ingredient for B. lactis INL1 biomass production. Acidic culture conditions yielded superior results, and all tested factors significantly influenced SDC performance. These findings highlight the usefulness of optimization strategies to define effective production conditions for probiotic cultures.
publishDate 2025
dc.date.none.fl_str_mv 2025-04-08T13:40:02Z
2025-04-08T13:40:02Z
2025-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21956
https://www.sciencedirect.com/science/article/abs/pii/S0958694624003017
0958-6946
1879-0143
https://doi.org/10.1016/j.idairyj.2024.106181
url http://hdl.handle.net/20.500.12123/21956
https://www.sciencedirect.com/science/article/abs/pii/S0958694624003017
https://doi.org/10.1016/j.idairyj.2024.106181
identifier_str_mv 0958-6946
1879-0143
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNAIyAV-1130032/AR./Tecnologías de transformación de alimentos.
info:eu-repograntAgreement/INTA/PNAIyAV-1130033/AR./Tecnologías de preservación de alimentos y de aprovechamiento de subproductos.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv International Dairy Journal 163 : 106181. (April 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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