A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants
- Autores
- Bermudez Salazar, Luisa; del Pozo, Talía; Lira, Bruno Silvestre; Godoy, Fabiana de; Boos, Irene; Romano, Cecilia; Previtali, Viola; Almeida, Juliana; Brehelin, Claire; Asis, Ramón; Quadrana, Leandro Daniel; Demarco, Diego; Alseekh, Saleh; Salinas Gamboa, Rigel; Perez-Flores, Laura; Dominguez, Pia Guadalupe; Rothan, Christophe; Fernie, Alisdair R.; Gonzalez, Maurıcio; Stocker, Achim; Hemmerle, Andreas; Clausen, Mads Hartving; Carrari, Fernando; Rossi, Magdalena
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.
Instituto de Biotecnología
Fil: Bermudez Salazar, Luisa. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lira, Bruno Silvestre. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil
Fil: Godoy, Fabiana de. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil
Fil: Boos, Irene. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; Dinamarca
Fil: Romano, Cecilia. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; Dinamarca
Fil: Previtali, Viola. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; Dinamarca
Fil: Almeida, Juliana. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil
Fil: Brehelin, Claire. Centre National de la Recherche Scientifique. Laboratory of Membrane Biogenesis; Francia
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Demarco, Diego. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil
Fil: Alseekh, Saleh. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Salinas Gamboa, Rigel. Universidad Autónoma Metropolitana. Laboratorio de Fisiología, Bioquímica y Biología Molecular de Plantas. Departamento de Ciencias de la Salud; México
Fil: Perez-Flores, Laura. Universidad Autónoma Metropolitana. Laboratorio de Fisiología, Bioquímica y Biología Molecular de Plantas. Departamento de Ciencias de la Salud; México
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rothan, Christophe. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; Francia
Fil: Fernie, Alisdair R. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Gonzalez, Maurıcio. Universidad de Chile. Laboratorio de Genética Molecular Vegetal; Chile
Fil: Stocker, Achim. University of Berne. Department of Chemistry and Biochemistry; Suiza
Fil: Hemmerle, Andreas. University of Berne. Department of Chemistry and Biochemistry; Suiza
Fil: Clausen, Mads Hartving. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; Dinamarca
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil
Fil: Rossi, Magdalena. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil - Fuente
- Plant and Cell Physiology 59 (11) : 2188-2203 (Noviembre 2018)
- Materia
-
Tomatoes
Tocopherols
Chloroplasts
Proteins
Tomate
Tocoferoles
Cloroplasto
Proteínas - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4819
Ver los metadatos del registro completo
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A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plantsBermudez Salazar, Luisadel Pozo, TalíaLira, Bruno SilvestreGodoy, Fabiana deBoos, IreneRomano, CeciliaPrevitali, ViolaAlmeida, JulianaBrehelin, ClaireAsis, RamónQuadrana, Leandro DanielDemarco, DiegoAlseekh, SalehSalinas Gamboa, RigelPerez-Flores, LauraDominguez, Pia GuadalupeRothan, ChristopheFernie, Alisdair R.Gonzalez, MaurıcioStocker, AchimHemmerle, AndreasClausen, Mads HartvingCarrari, FernandoRossi, MagdalenaTomatoesTocopherolsChloroplastsProteinsTomateTocoferolesCloroplastoProteínasTocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.Instituto de BiotecnologíaFil: Bermudez Salazar, Luisa. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lira, Bruno Silvestre. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; BrasilFil: Godoy, Fabiana de. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; BrasilFil: Boos, Irene. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; DinamarcaFil: Romano, Cecilia. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; DinamarcaFil: Previtali, Viola. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; DinamarcaFil: Almeida, Juliana. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; BrasilFil: Brehelin, Claire. Centre National de la Recherche Scientifique. Laboratory of Membrane Biogenesis; FranciaFil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Demarco, Diego. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; BrasilFil: Alseekh, Saleh. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Salinas Gamboa, Rigel. Universidad Autónoma Metropolitana. Laboratorio de Fisiología, Bioquímica y Biología Molecular de Plantas. Departamento de Ciencias de la Salud; MéxicoFil: Perez-Flores, Laura. Universidad Autónoma Metropolitana. Laboratorio de Fisiología, Bioquímica y Biología Molecular de Plantas. Departamento de Ciencias de la Salud; MéxicoFil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rothan, Christophe. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; FranciaFil: Fernie, Alisdair R. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Gonzalez, Maurıcio. Universidad de Chile. Laboratorio de Genética Molecular Vegetal; ChileFil: Stocker, Achim. University of Berne. Department of Chemistry and Biochemistry; SuizaFil: Hemmerle, Andreas. University of Berne. Department of Chemistry and Biochemistry; SuizaFil: Clausen, Mads Hartving. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; DinamarcaFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; BrasilFil: Rossi, Magdalena. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; BrasilOxford University Press2019-04-04T17:08:20Z2019-04-04T17:08:20Z2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://academic.oup.com/pcp/article/59/11/2188/5101312http://hdl.handle.net/20.500.12123/48191471-9053https://doi.org/10.1093/pcp/pcy191Plant and Cell Physiology 59 (11) : 2188-2203 (Noviembre 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:54Zoai:localhost:20.500.12123/4819instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:55.049INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants |
| title |
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants |
| spellingShingle |
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants Bermudez Salazar, Luisa Tomatoes Tocopherols Chloroplasts Proteins Tomate Tocoferoles Cloroplasto Proteínas |
| title_short |
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants |
| title_full |
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants |
| title_fullStr |
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants |
| title_full_unstemmed |
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants |
| title_sort |
A tomato tocopherol-binding protein sheds light on intracellular a-tocopherol metabolism in plants |
| dc.creator.none.fl_str_mv |
Bermudez Salazar, Luisa del Pozo, Talía Lira, Bruno Silvestre Godoy, Fabiana de Boos, Irene Romano, Cecilia Previtali, Viola Almeida, Juliana Brehelin, Claire Asis, Ramón Quadrana, Leandro Daniel Demarco, Diego Alseekh, Saleh Salinas Gamboa, Rigel Perez-Flores, Laura Dominguez, Pia Guadalupe Rothan, Christophe Fernie, Alisdair R. Gonzalez, Maurıcio Stocker, Achim Hemmerle, Andreas Clausen, Mads Hartving Carrari, Fernando Rossi, Magdalena |
| author |
Bermudez Salazar, Luisa |
| author_facet |
Bermudez Salazar, Luisa del Pozo, Talía Lira, Bruno Silvestre Godoy, Fabiana de Boos, Irene Romano, Cecilia Previtali, Viola Almeida, Juliana Brehelin, Claire Asis, Ramón Quadrana, Leandro Daniel Demarco, Diego Alseekh, Saleh Salinas Gamboa, Rigel Perez-Flores, Laura Dominguez, Pia Guadalupe Rothan, Christophe Fernie, Alisdair R. Gonzalez, Maurıcio Stocker, Achim Hemmerle, Andreas Clausen, Mads Hartving Carrari, Fernando Rossi, Magdalena |
| author_role |
author |
| author2 |
del Pozo, Talía Lira, Bruno Silvestre Godoy, Fabiana de Boos, Irene Romano, Cecilia Previtali, Viola Almeida, Juliana Brehelin, Claire Asis, Ramón Quadrana, Leandro Daniel Demarco, Diego Alseekh, Saleh Salinas Gamboa, Rigel Perez-Flores, Laura Dominguez, Pia Guadalupe Rothan, Christophe Fernie, Alisdair R. Gonzalez, Maurıcio Stocker, Achim Hemmerle, Andreas Clausen, Mads Hartving Carrari, Fernando Rossi, Magdalena |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Tomatoes Tocopherols Chloroplasts Proteins Tomate Tocoferoles Cloroplasto Proteínas |
| topic |
Tomatoes Tocopherols Chloroplasts Proteins Tomate Tocoferoles Cloroplasto Proteínas |
| dc.description.none.fl_txt_mv |
Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism. Instituto de Biotecnología Fil: Bermudez Salazar, Luisa. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lira, Bruno Silvestre. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil Fil: Godoy, Fabiana de. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil Fil: Boos, Irene. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; Dinamarca Fil: Romano, Cecilia. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; Dinamarca Fil: Previtali, Viola. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; Dinamarca Fil: Almeida, Juliana. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil Fil: Brehelin, Claire. Centre National de la Recherche Scientifique. Laboratory of Membrane Biogenesis; Francia Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Demarco, Diego. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil Fil: Alseekh, Saleh. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Salinas Gamboa, Rigel. Universidad Autónoma Metropolitana. Laboratorio de Fisiología, Bioquímica y Biología Molecular de Plantas. Departamento de Ciencias de la Salud; México Fil: Perez-Flores, Laura. Universidad Autónoma Metropolitana. Laboratorio de Fisiología, Bioquímica y Biología Molecular de Plantas. Departamento de Ciencias de la Salud; México Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rothan, Christophe. Institut National de la Recherche Agronomique. Fruit Biology and Pathology Unit; Francia Fil: Fernie, Alisdair R. Max Planck Institute of Molecular Plant Physiology; Alemania Fil: Gonzalez, Maurıcio. Universidad de Chile. Laboratorio de Genética Molecular Vegetal; Chile Fil: Stocker, Achim. University of Berne. Department of Chemistry and Biochemistry; Suiza Fil: Hemmerle, Andreas. University of Berne. Department of Chemistry and Biochemistry; Suiza Fil: Clausen, Mads Hartving. Technical University of Denmark. Center for Nanomedicine and Theranostics. Department of Chemistry; Dinamarca Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil Fil: Rossi, Magdalena. Universidade de São Pablo. Departamento de Botânica. Instituto de Biociencias; Brasil |
| description |
Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-11 2019-04-04T17:08:20Z 2019-04-04T17:08:20Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://academic.oup.com/pcp/article/59/11/2188/5101312 http://hdl.handle.net/20.500.12123/4819 1471-9053 https://doi.org/10.1093/pcp/pcy191 |
| url |
https://academic.oup.com/pcp/article/59/11/2188/5101312 http://hdl.handle.net/20.500.12123/4819 https://doi.org/10.1093/pcp/pcy191 |
| identifier_str_mv |
1471-9053 |
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eng |
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eng |
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info:eu-repo/semantics/restrictedAccess |
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restrictedAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Oxford University Press |
| publisher.none.fl_str_mv |
Oxford University Press |
| dc.source.none.fl_str_mv |
Plant and Cell Physiology 59 (11) : 2188-2203 (Noviembre 2018) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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