A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants

Autores: Bermúdez, Luisa; Del Pozo, Talía; Silvestre Lira, Bruno; de Godoy, Fabiana; Boos, Irene; Romanò, Cecilia; Previtali, Viola; Almeida, Juliana; Bréhélin, Claire; Asis, Ramón; Quadrana, Leandro Daniel; Demarco, Diego Gabriel; Alseekh, Saleh; Salinas Gamboa, Rigel; Pérez-Flores, Laura; Dominguez, Pia Guadalupe; Rothan, Christophe; Fernie, Alisdair Robert; González, Maurício; Stocker, Achim; Hemmerle, Andreas; Clausen, Mads Hartving; Carrari, Fernando Oscar; Rossi, Magdalena
Año de publicación: 2018
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.
Fil: Bermúdez, Luisa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Del Pozo, Talía. Universidad Mayor; Chile
Fil: Silvestre Lira, Bruno. Universidade de Sao Paulo; Brasil
Fil: de Godoy, Fabiana. Universidade de Sao Paulo; Brasil
Fil: Boos, Irene. Technical University of Denmark; Dinamarca
Fil: Romanò, Cecilia. Technical University of Denmark; Dinamarca
Fil: Previtali, Viola. Technical University of Denmark; Dinamarca
Fil: Almeida, Juliana. Universidade de Sao Paulo; Brasil
Fil: Bréhélin, Claire. Laboratoire de Biogénèse Membranaire; Francia
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Demarco, Diego Gabriel. Universidade de Sao Paulo; Brasil
Fil: Alseekh, Saleh. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Salinas Gamboa, Rigel. Universidad Autónoma Metropolitana - Iztapalapa; México
Fil: Pérez-Flores, Laura. Universidad Autónoma Metropolitana - Iztapalapa; México
Fil: Dominguez, Pia Guadalupe. Comisión Nacional de Investigación Científica y Tecnológica; Chile. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Rothan, Christophe. Biologie Du Fruit Et Pathologie; Francia
Fil: Fernie, Alisdair Robert. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: González, Maurício. Universidad de Chile; Chile
Fil: Stocker, Achim. University of Bern; Suiza
Fil: Hemmerle, Andreas. University of Bern; Suiza
Fil: Clausen, Mads Hartving. Technical University of Denmark; Dinamarca
Fil: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Rossi, Magdalena. Universidade de Sao Paulo; Brasil
Materia: TOCOPHEROL
TOMATO
TOCOPHEROL TRANSPORT
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/104898

Acceder

id	CONICETDig_c6eafdcdbfefe4c062f92ec1814631ef
oai_identifier_str	oai:ri.conicet.gov.ar:11336/104898
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plantsBermúdez, LuisaDel Pozo, TalíaSilvestre Lira, Brunode Godoy, FabianaBoos, IreneRomanò, CeciliaPrevitali, ViolaAlmeida, JulianaBréhélin, ClaireAsis, RamónQuadrana, Leandro DanielDemarco, Diego GabrielAlseekh, SalehSalinas Gamboa, RigelPérez-Flores, LauraDominguez, Pia GuadalupeRothan, ChristopheFernie, Alisdair RobertGonzález, MaurícioStocker, AchimHemmerle, AndreasClausen, Mads HartvingCarrari, Fernando OscarRossi, MagdalenaTOCOPHEROLTOMATOTOCOPHEROL TRANSPORThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.Fil: Bermúdez, Luisa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Del Pozo, Talía. Universidad Mayor; ChileFil: Silvestre Lira, Bruno. Universidade de Sao Paulo; BrasilFil: de Godoy, Fabiana. Universidade de Sao Paulo; BrasilFil: Boos, Irene. Technical University of Denmark; DinamarcaFil: Romanò, Cecilia. Technical University of Denmark; DinamarcaFil: Previtali, Viola. Technical University of Denmark; DinamarcaFil: Almeida, Juliana. Universidade de Sao Paulo; BrasilFil: Bréhélin, Claire. Laboratoire de Biogénèse Membranaire; FranciaFil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Demarco, Diego Gabriel. Universidade de Sao Paulo; BrasilFil: Alseekh, Saleh. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Salinas Gamboa, Rigel. Universidad Autónoma Metropolitana - Iztapalapa; MéxicoFil: Pérez-Flores, Laura. Universidad Autónoma Metropolitana - Iztapalapa; MéxicoFil: Dominguez, Pia Guadalupe. Comisión Nacional de Investigación Científica y Tecnológica; Chile. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Rothan, Christophe. Biologie Du Fruit Et Pathologie; FranciaFil: Fernie, Alisdair Robert. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: González, Maurício. Universidad de Chile; ChileFil: Stocker, Achim. University of Bern; SuizaFil: Hemmerle, Andreas. University of Bern; SuizaFil: Clausen, Mads Hartving. Technical University of Denmark; DinamarcaFil: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Rossi, Magdalena. Universidade de Sao Paulo; BrasilNLM (Medline)2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104898Bermúdez, Luisa; Del Pozo, Talía; Silvestre Lira, Bruno; de Godoy, Fabiana; Boos, Irene; et al.; A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants; NLM (Medline); Plant & Cell Physiology; 59; 11; 11-2018; 2188-22031471-90531471-9053CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/pcp/pcy191info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/30239816info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:49Zoai:ri.conicet.gov.ar:11336/104898instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:50.017CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants
title	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants
spellingShingle	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants Bermúdez, Luisa TOCOPHEROL TOMATO TOCOPHEROL TRANSPORT
title_short	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants
title_full	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants
title_fullStr	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants
title_full_unstemmed	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants
title_sort	A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants
dc.creator.none.fl_str_mv	Bermúdez, Luisa Del Pozo, Talía Silvestre Lira, Bruno de Godoy, Fabiana Boos, Irene Romanò, Cecilia Previtali, Viola Almeida, Juliana Bréhélin, Claire Asis, Ramón Quadrana, Leandro Daniel Demarco, Diego Gabriel Alseekh, Saleh Salinas Gamboa, Rigel Pérez-Flores, Laura Dominguez, Pia Guadalupe Rothan, Christophe Fernie, Alisdair Robert González, Maurício Stocker, Achim Hemmerle, Andreas Clausen, Mads Hartving Carrari, Fernando Oscar Rossi, Magdalena
author	Bermúdez, Luisa
author_facet	Bermúdez, Luisa Del Pozo, Talía Silvestre Lira, Bruno de Godoy, Fabiana Boos, Irene Romanò, Cecilia Previtali, Viola Almeida, Juliana Bréhélin, Claire Asis, Ramón Quadrana, Leandro Daniel Demarco, Diego Gabriel Alseekh, Saleh Salinas Gamboa, Rigel Pérez-Flores, Laura Dominguez, Pia Guadalupe Rothan, Christophe Fernie, Alisdair Robert González, Maurício Stocker, Achim Hemmerle, Andreas Clausen, Mads Hartving Carrari, Fernando Oscar Rossi, Magdalena
author_role	author
author2	Del Pozo, Talía Silvestre Lira, Bruno de Godoy, Fabiana Boos, Irene Romanò, Cecilia Previtali, Viola Almeida, Juliana Bréhélin, Claire Asis, Ramón Quadrana, Leandro Daniel Demarco, Diego Gabriel Alseekh, Saleh Salinas Gamboa, Rigel Pérez-Flores, Laura Dominguez, Pia Guadalupe Rothan, Christophe Fernie, Alisdair Robert González, Maurício Stocker, Achim Hemmerle, Andreas Clausen, Mads Hartving Carrari, Fernando Oscar Rossi, Magdalena
author2_role	author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	TOCOPHEROL TOMATO TOCOPHEROL TRANSPORT
topic	TOCOPHEROL TOMATO TOCOPHEROL TRANSPORT
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism. Fil: Bermúdez, Luisa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Del Pozo, Talía. Universidad Mayor; Chile Fil: Silvestre Lira, Bruno. Universidade de Sao Paulo; Brasil Fil: de Godoy, Fabiana. Universidade de Sao Paulo; Brasil Fil: Boos, Irene. Technical University of Denmark; Dinamarca Fil: Romanò, Cecilia. Technical University of Denmark; Dinamarca Fil: Previtali, Viola. Technical University of Denmark; Dinamarca Fil: Almeida, Juliana. Universidade de Sao Paulo; Brasil Fil: Bréhélin, Claire. Laboratoire de Biogénèse Membranaire; Francia Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Demarco, Diego Gabriel. Universidade de Sao Paulo; Brasil Fil: Alseekh, Saleh. Max Planck Institute Of Molecular Plant Physiology; Alemania Fil: Salinas Gamboa, Rigel. Universidad Autónoma Metropolitana - Iztapalapa; México Fil: Pérez-Flores, Laura. Universidad Autónoma Metropolitana - Iztapalapa; México Fil: Dominguez, Pia Guadalupe. Comisión Nacional de Investigación Científica y Tecnológica; Chile. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Rothan, Christophe. Biologie Du Fruit Et Pathologie; Francia Fil: Fernie, Alisdair Robert. Max Planck Institute Of Molecular Plant Physiology; Alemania Fil: González, Maurício. Universidad de Chile; Chile Fil: Stocker, Achim. University of Bern; Suiza Fil: Hemmerle, Andreas. University of Bern; Suiza Fil: Clausen, Mads Hartving. Technical University of Denmark; Dinamarca Fil: Carrari, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Rossi, Magdalena. Universidade de Sao Paulo; Brasil
description	Tocopherols are non-polar compounds synthesized in the plastids, which function as major antioxidants of the plant cells and are essential in the human diet. Both the intermediates and final products of the tocopherol biosynthetic pathway must cross plastid membranes to reach their sites of action. So far, no protein with tocopherol binding activity has been reported in plants. Here, we demonstrated that the tomato SlTBP protein is targeted to chloroplasts and able to bind α-tocopherol. SlTBP-knockdown tomato plants exhibited reduced levels of tocopherol in both leaves and fruits. Several tocopherol deficiency phenotypes were apparent in the transgenic lines, such as alterations in photosynthetic parameters, dramatic distortion of thylakoid membranes and significant variations in the lipid profile. These results, along with the altered expression of genes related to photosynthesis, and tetrapyrrole, lipid, isoprenoid, inositol/phosphoinositide and redox metabolism, suggest that SlTBP may act in conducting tocopherol (or its biosynthetic intermediates) between the plastid compartments and/or at the interface between chloroplast and endoplasmic reticulum membranes, affecting interorganellar lipid metabolism.
publishDate	2018
dc.date.none.fl_str_mv	2018-11
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/104898 Bermúdez, Luisa; Del Pozo, Talía; Silvestre Lira, Bruno; de Godoy, Fabiana; Boos, Irene; et al.; A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants; NLM (Medline); Plant & Cell Physiology; 59; 11; 11-2018; 2188-2203 1471-9053 1471-9053 CONICET Digital CONICET
url	http://hdl.handle.net/11336/104898
identifier_str_mv	Bermúdez, Luisa; Del Pozo, Talía; Silvestre Lira, Bruno; de Godoy, Fabiana; Boos, Irene; et al.; A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants; NLM (Medline); Plant & Cell Physiology; 59; 11; 11-2018; 2188-2203 1471-9053 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1093/pcp/pcy191 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/30239816
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	NLM (Medline)
publisher.none.fl_str_mv	NLM (Medline)
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1842268997031034880
score	13.13397

A tomato tocopherol binding protein sheds light on intracellular α-tocopherol metabolism in plants

Publicaciones similares