Comparison of two serological diagnosis tests for bovine paratuberculosis

Autores
Tieri, Silvina; Morsella, Claudia Graciela; Mendez, Laura Luján; Vasini, B.; Garro, Carlos Javier; Paolicchi, Fernando
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the disease. This research aimed to evaluate two different ELISAs: (a) an “in-house” ELISA with a sonicated antigen obtained from a MAP I47 strain, and (b) a commercial ELISA. In total, the evaluated sample consisted of 394 bovine serum samples from 12 farms in Argentina with high (5–9%) and low (≤ 0.05%) prevalence of PTBC. The evaluation of the new antigen (2.5 µg/mL) was against a 1:50 dilution of the M. phlei faced sera. The cut-off point, sensitivity, and specificity determinations of both techniques were by ROC curve analysis. The area under the curve for the I47 ELISA was 0.9 (CI 95%, 0.93–0.97). With a cut-off point of 8.8%, the sensitivity was 84.3% and the specificity 96.6%. The agreement between both techniques was 0.7 (CI 95%, 0.6–0.8). These results indicate a high discriminative capacity to differentiate positive and negative bovine sera of MAP infection with the I47 ELISA. This result would represent an advantage to dispense with the imported kit.
EEA Balcarce
Fil: Tieri, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Balcarce; Argentina
Fil: Tieri, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Vasini, B. University of North Texas Health Science Center; Estados Unidos
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fuente
Veterinary Research Communications 48 : 2645-2650, (2024)
Materia
Ganado Bovino
Enfermedades de los Animales
Inmunodiagnóstico
Cattle
Animal Diseases
Paratuberculosis
Mycobacterium avium subsp. paratuberculosis
Immunodiagnosis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21053
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21053
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Comparison of two serological diagnosis tests for bovine paratuberculosisTieri, SilvinaMorsella, Claudia GracielaMendez, Laura LujánVasini, B.Garro, Carlos JavierPaolicchi, FernandoGanado BovinoEnfermedades de los AnimalesInmunodiagnósticoCattleAnimal DiseasesParatuberculosisMycobacterium avium subsp. paratuberculosisImmunodiagnosisMycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the disease. This research aimed to evaluate two different ELISAs: (a) an “in-house” ELISA with a sonicated antigen obtained from a MAP I47 strain, and (b) a commercial ELISA. In total, the evaluated sample consisted of 394 bovine serum samples from 12 farms in Argentina with high (5–9%) and low (≤ 0.05%) prevalence of PTBC. The evaluation of the new antigen (2.5 µg/mL) was against a 1:50 dilution of the M. phlei faced sera. The cut-off point, sensitivity, and specificity determinations of both techniques were by ROC curve analysis. The area under the curve for the I47 ELISA was 0.9 (CI 95%, 0.93–0.97). With a cut-off point of 8.8%, the sensitivity was 84.3% and the specificity 96.6%. The agreement between both techniques was 0.7 (CI 95%, 0.6–0.8). These results indicate a high discriminative capacity to differentiate positive and negative bovine sera of MAP infection with the I47 ELISA. This result would represent an advantage to dispense with the imported kit.EEA BalcarceFil: Tieri, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Balcarce; ArgentinaFil: Tieri, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Vasini, B. University of North Texas Health Science Center; Estados UnidosFil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Springer2025-01-22T16:08:28Z2025-01-22T16:08:28Z2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21053https://link.springer.com/article/10.1007/s11259-024-10363-70165-7380 (print)1573-7446 (online)https://doi.org/10.1007/s11259-024-10363-7Veterinary Research Communications 48 : 2645-2650, (2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-18T10:09:54Zoai:localhost:20.500.12123/21053instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:09:54.973INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comparison of two serological diagnosis tests for bovine paratuberculosis
title Comparison of two serological diagnosis tests for bovine paratuberculosis
spellingShingle Comparison of two serological diagnosis tests for bovine paratuberculosis
Tieri, Silvina
Ganado Bovino
Enfermedades de los Animales
Inmunodiagnóstico
Cattle
Animal Diseases
Paratuberculosis
Mycobacterium avium subsp. paratuberculosis
Immunodiagnosis
title_short Comparison of two serological diagnosis tests for bovine paratuberculosis
title_full Comparison of two serological diagnosis tests for bovine paratuberculosis
title_fullStr Comparison of two serological diagnosis tests for bovine paratuberculosis
title_full_unstemmed Comparison of two serological diagnosis tests for bovine paratuberculosis
title_sort Comparison of two serological diagnosis tests for bovine paratuberculosis
dc.creator.none.fl_str_mv Tieri, Silvina
Morsella, Claudia Graciela
Mendez, Laura Luján
Vasini, B.
Garro, Carlos Javier
Paolicchi, Fernando
author Tieri, Silvina
author_facet Tieri, Silvina
Morsella, Claudia Graciela
Mendez, Laura Luján
Vasini, B.
Garro, Carlos Javier
Paolicchi, Fernando
author_role author
author2 Morsella, Claudia Graciela
Mendez, Laura Luján
Vasini, B.
Garro, Carlos Javier
Paolicchi, Fernando
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Inmunodiagnóstico
Cattle
Animal Diseases
Paratuberculosis
Mycobacterium avium subsp. paratuberculosis
Immunodiagnosis
topic Ganado Bovino
Enfermedades de los Animales
Inmunodiagnóstico
Cattle
Animal Diseases
Paratuberculosis
Mycobacterium avium subsp. paratuberculosis
Immunodiagnosis
dc.description.none.fl_txt_mv Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the disease. This research aimed to evaluate two different ELISAs: (a) an “in-house” ELISA with a sonicated antigen obtained from a MAP I47 strain, and (b) a commercial ELISA. In total, the evaluated sample consisted of 394 bovine serum samples from 12 farms in Argentina with high (5–9%) and low (≤ 0.05%) prevalence of PTBC. The evaluation of the new antigen (2.5 µg/mL) was against a 1:50 dilution of the M. phlei faced sera. The cut-off point, sensitivity, and specificity determinations of both techniques were by ROC curve analysis. The area under the curve for the I47 ELISA was 0.9 (CI 95%, 0.93–0.97). With a cut-off point of 8.8%, the sensitivity was 84.3% and the specificity 96.6%. The agreement between both techniques was 0.7 (CI 95%, 0.6–0.8). These results indicate a high discriminative capacity to differentiate positive and negative bovine sera of MAP infection with the I47 ELISA. This result would represent an advantage to dispense with the imported kit.
EEA Balcarce
Fil: Tieri, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Balcarce; Argentina
Fil: Tieri, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Mendez, Laura Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Vasini, B. University of North Texas Health Science Center; Estados Unidos
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
description Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of paratuberculosis (PTBC), a chronic infectious granulomatous enteritis of ruminants. The PTBC diagnosis with commercial ELISA has limitations in sensitivity and specificity, and its results depend on the state of progress of the disease. This research aimed to evaluate two different ELISAs: (a) an “in-house” ELISA with a sonicated antigen obtained from a MAP I47 strain, and (b) a commercial ELISA. In total, the evaluated sample consisted of 394 bovine serum samples from 12 farms in Argentina with high (5–9%) and low (≤ 0.05%) prevalence of PTBC. The evaluation of the new antigen (2.5 µg/mL) was against a 1:50 dilution of the M. phlei faced sera. The cut-off point, sensitivity, and specificity determinations of both techniques were by ROC curve analysis. The area under the curve for the I47 ELISA was 0.9 (CI 95%, 0.93–0.97). With a cut-off point of 8.8%, the sensitivity was 84.3% and the specificity 96.6%. The agreement between both techniques was 0.7 (CI 95%, 0.6–0.8). These results indicate a high discriminative capacity to differentiate positive and negative bovine sera of MAP infection with the I47 ELISA. This result would represent an advantage to dispense with the imported kit.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
2025-01-22T16:08:28Z
2025-01-22T16:08:28Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21053
https://link.springer.com/article/10.1007/s11259-024-10363-7
0165-7380 (print)
1573-7446 (online)
https://doi.org/10.1007/s11259-024-10363-7
url http://hdl.handle.net/20.500.12123/21053
https://link.springer.com/article/10.1007/s11259-024-10363-7
https://doi.org/10.1007/s11259-024-10363-7
identifier_str_mv 0165-7380 (print)
1573-7446 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Veterinary Research Communications 48 : 2645-2650, (2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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