Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
- Autores
- da Silva de Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; da Rocha, David Gitirana; Dias da Silva, Wilmar; Lasunskaia, Elena B.
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.
Instituto de Biotecnología
Fil: da Silva de Souza, Giliane. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil
Fil: Rodriguez, Ana Barbara F. Universidade Estadual do Norte Fluminense. Laboratory of Animal Morphology and Pathology; Brasil
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ribeiro, Efranci Seliprandy. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil
Fil: Oelemann, Walter M. R. Federal University of Rio de Janeiro. Paulo de Goes Microbiology Institute; Brasil
Fil: da Rocha, David Gitirana. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil
Fil: Dias da Silva, Wilmar. Butantan Institute. Immunochemistry Laboratory; Brasil
Fil: Lasunskaia, Elena B. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil - Fuente
- Pathogens and Disease 76 (6): fty063 (Agosto 2018)
- Materia
-
Paratuberculosis
Técnicas Inmunológicas
Heces
Mycobacterium avium
Inmunodiagnóstico
Ganado bovino
Immunological Techniques
Faeces
Immunodiagnosis
Cattle
Fecal Biomarker
Biomarcador Fecal - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4821
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Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattleda Silva de Souza, GilianeRodriguez, Ana Barbara F.Romano, Maria IsabelRibeiro, Efranci SeliprandyOelemann, Walter M. R.da Rocha, David GitiranaDias da Silva, WilmarLasunskaia, Elena B.ParatuberculosisTécnicas InmunológicasHecesMycobacterium aviumInmunodiagnósticoGanado bovinoImmunological TechniquesFaecesImmunodiagnosisCattleFecal BiomarkerBiomarcador FecalParatuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.Instituto de BiotecnologíaFil: da Silva de Souza, Giliane. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; BrasilFil: Rodriguez, Ana Barbara F. Universidade Estadual do Norte Fluminense. Laboratory of Animal Morphology and Pathology; BrasilFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Ribeiro, Efranci Seliprandy. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; BrasilFil: Oelemann, Walter M. R. Federal University of Rio de Janeiro. Paulo de Goes Microbiology Institute; BrasilFil: da Rocha, David Gitirana. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; BrasilFil: Dias da Silva, Wilmar. Butantan Institute. Immunochemistry Laboratory; BrasilFil: Lasunskaia, Elena B. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; BrasilOxford University Press2019-04-05T10:53:25Z2019-04-05T10:53:25Z2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://academic.oup.com/femspd/article-abstract/76/6/fty063/5057472?redirectedFrom=fulltexthttp://hdl.handle.net/20.500.12123/48212049-632Xhttps://doi.org/10.1093/femspd/fty063Pathogens and Disease 76 (6): fty063 (Agosto 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-18T10:07:31Zoai:localhost:20.500.12123/4821instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:07:31.896INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title |
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| spellingShingle |
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle da Silva de Souza, Giliane Paratuberculosis Técnicas Inmunológicas Heces Mycobacterium avium Inmunodiagnóstico Ganado bovino Immunological Techniques Faeces Immunodiagnosis Cattle Fecal Biomarker Biomarcador Fecal |
| title_short |
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_full |
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_fullStr |
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_full_unstemmed |
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| title_sort |
Identification of the Apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
| dc.creator.none.fl_str_mv |
da Silva de Souza, Giliane Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. da Rocha, David Gitirana Dias da Silva, Wilmar Lasunskaia, Elena B. |
| author |
da Silva de Souza, Giliane |
| author_facet |
da Silva de Souza, Giliane Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. da Rocha, David Gitirana Dias da Silva, Wilmar Lasunskaia, Elena B. |
| author_role |
author |
| author2 |
Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. da Rocha, David Gitirana Dias da Silva, Wilmar Lasunskaia, Elena B. |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Paratuberculosis Técnicas Inmunológicas Heces Mycobacterium avium Inmunodiagnóstico Ganado bovino Immunological Techniques Faeces Immunodiagnosis Cattle Fecal Biomarker Biomarcador Fecal |
| topic |
Paratuberculosis Técnicas Inmunológicas Heces Mycobacterium avium Inmunodiagnóstico Ganado bovino Immunological Techniques Faeces Immunodiagnosis Cattle Fecal Biomarker Biomarcador Fecal |
| dc.description.none.fl_txt_mv |
Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis. Instituto de Biotecnología Fil: da Silva de Souza, Giliane. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil Fil: Rodriguez, Ana Barbara F. Universidade Estadual do Norte Fluminense. Laboratory of Animal Morphology and Pathology; Brasil Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Ribeiro, Efranci Seliprandy. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil Fil: Oelemann, Walter M. R. Federal University of Rio de Janeiro. Paulo de Goes Microbiology Institute; Brasil Fil: da Rocha, David Gitirana. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil Fil: Dias da Silva, Wilmar. Butantan Institute. Immunochemistry Laboratory; Brasil Fil: Lasunskaia, Elena B. Universidade Estadual do Norte Fluminense. Laboratory of Biology of Recognition; Brasil |
| description |
Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-08 2019-04-05T10:53:25Z 2019-04-05T10:53:25Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
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https://academic.oup.com/femspd/article-abstract/76/6/fty063/5057472?redirectedFrom=fulltext http://hdl.handle.net/20.500.12123/4821 2049-632X https://doi.org/10.1093/femspd/fty063 |
| url |
https://academic.oup.com/femspd/article-abstract/76/6/fty063/5057472?redirectedFrom=fulltext http://hdl.handle.net/20.500.12123/4821 https://doi.org/10.1093/femspd/fty063 |
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2049-632X |
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eng |
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eng |
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restrictedAccess |
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application/pdf |
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Oxford University Press |
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Oxford University Press |
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Pathogens and Disease 76 (6): fty063 (Agosto 2018) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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