Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission

Autores
Pérez, Agustina Ericlee; Guillemi, Eliana Carolina; Abuin-Denis, Lianet; Piloto-Sardiñas, Elianne; Obregón, Dasiel; Pin Viso, Natalia Daniela; Sarmiento, Nestor Fabian; Cabezas-Cruz, Alejandro; Farber, Marisa Diana
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cattle tick Rhipicephalus microplus is a vector for Anaplasma marginale in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study focused on how A. marginale infection affects the bacterial community within the salivary glands and ovaries of R. microplus. Engorged female ticks collected from cattle in a disease-endemic area of Argentina were further classified based on whether they were infected with A. marginale by targeting the msp1β gene through PCR diagnosis in tissue samples. We included negative control samples throughout the study. We analyzed the bacterial communities in tick tissues by sequencing the 16S rRNA V3-V4 region. Our results revealed significant differences in community composition between infected and uninfected samples. Sphingomonadaceae was identified as a predominant taxon in uninfected salivary glands and ovaries. Additionally, we constructed co-occurrence networks to study interactions within the microbial communities. It is noteworthy that A. marginale infection led to an increase in network complexity in the salivary glands, exerting the opposite effect on the ovaries. These findings reinforced the hypothesis that A. marginale impacts the microbiota of R. microplus at an organ-specific level.
Instituto de Biotecnología
Fil: Pérez, Agustina Ericlee. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Pérez, Agustina Ericlee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Abuin-Denis, Lianet. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia
Fil: Abuin-Denis, Lianet. Center for Genetic Engineering and Biotechnology. Animal Biotechnology Department; Cuba
Fil: Piloto-Sardiñas, Elianne. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia
Fil: Piloto-Sardiñas, Elianne. National Center for Animal and Plant Health. Direction of Animal Health; Cuba
Fil: Obregón, Dasiel. University of Guelph. School of Environmental Sciences; Canadá
Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Cabezas-Cruz, Alejandro. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia
Fil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Ticks and Tick-borne Diseases 16 (5) : 102522 (September 2025)
Materia
Anaplasma marginale
Microbiomes
Ovaries
Pathogens
Rhipicephalus
Salivary Glands
Microbiomas
Ovarios
Organismos Patógenos
Glándulas Salivales
Rhipicephalus microplus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23644
Acceder
id INTADig_ab96eb1a95aca40d7ff9f199f56cf8d0
oai_identifier_str oai:localhost:20.500.12123/23644
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmissionPérez, Agustina EricleeGuillemi, Eliana CarolinaAbuin-Denis, LianetPiloto-Sardiñas, ElianneObregón, DasielPin Viso, Natalia DanielaSarmiento, Nestor FabianCabezas-Cruz, AlejandroFarber, Marisa DianaAnaplasma marginaleMicrobiomesOvariesPathogensRhipicephalusSalivary GlandsMicrobiomasOvariosOrganismos PatógenosGlándulas SalivalesRhipicephalus microplusThe cattle tick Rhipicephalus microplus is a vector for Anaplasma marginale in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study focused on how A. marginale infection affects the bacterial community within the salivary glands and ovaries of R. microplus. Engorged female ticks collected from cattle in a disease-endemic area of Argentina were further classified based on whether they were infected with A. marginale by targeting the msp1β gene through PCR diagnosis in tissue samples. We included negative control samples throughout the study. We analyzed the bacterial communities in tick tissues by sequencing the 16S rRNA V3-V4 region. Our results revealed significant differences in community composition between infected and uninfected samples. Sphingomonadaceae was identified as a predominant taxon in uninfected salivary glands and ovaries. Additionally, we constructed co-occurrence networks to study interactions within the microbial communities. It is noteworthy that A. marginale infection led to an increase in network complexity in the salivary glands, exerting the opposite effect on the ovaries. These findings reinforced the hypothesis that A. marginale impacts the microbiota of R. microplus at an organ-specific level.Instituto de BiotecnologíaFil: Pérez, Agustina Ericlee. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Pérez, Agustina Ericlee. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Abuin-Denis, Lianet. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; FranciaFil: Abuin-Denis, Lianet. Center for Genetic Engineering and Biotechnology. Animal Biotechnology Department; CubaFil: Piloto-Sardiñas, Elianne. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; FranciaFil: Piloto-Sardiñas, Elianne. National Center for Animal and Plant Health. Direction of Animal Health; CubaFil: Obregón, Dasiel. University of Guelph. School of Environmental Sciences; CanadáFil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Cabezas-Cruz, Alejandro. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; FranciaFil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Farber, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2025-09-02T10:08:58Z2025-09-02T10:08:58Z2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23644https://www.sciencedirect.com/science/article/pii/S1877959X2500086X1877-959Xhttps://doi.org/10.1016/j.ttbdis.2025.102522Ticks and Tick-borne Diseases 16 (5) : 102522 (September 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:16Zoai:localhost:20.500.12123/23644instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:16.585INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
title Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
spellingShingle Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
Pérez, Agustina Ericlee
Anaplasma marginale
Microbiomes
Ovaries
Pathogens
Rhipicephalus
Salivary Glands
Microbiomas
Ovarios
Organismos Patógenos
Glándulas Salivales
Rhipicephalus microplus
title_short Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
title_full Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
title_fullStr Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
title_full_unstemmed Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
title_sort Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission
dc.creator.none.fl_str_mv Pérez, Agustina Ericlee
Guillemi, Eliana Carolina
Abuin-Denis, Lianet
Piloto-Sardiñas, Elianne
Obregón, Dasiel
Pin Viso, Natalia Daniela
Sarmiento, Nestor Fabian
Cabezas-Cruz, Alejandro
Farber, Marisa Diana
author Pérez, Agustina Ericlee
author_facet Pérez, Agustina Ericlee
Guillemi, Eliana Carolina
Abuin-Denis, Lianet
Piloto-Sardiñas, Elianne
Obregón, Dasiel
Pin Viso, Natalia Daniela
Sarmiento, Nestor Fabian
Cabezas-Cruz, Alejandro
Farber, Marisa Diana
author_role author
author2 Guillemi, Eliana Carolina
Abuin-Denis, Lianet
Piloto-Sardiñas, Elianne
Obregón, Dasiel
Pin Viso, Natalia Daniela
Sarmiento, Nestor Fabian
Cabezas-Cruz, Alejandro
Farber, Marisa Diana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anaplasma marginale
Microbiomes
Ovaries
Pathogens
Rhipicephalus
Salivary Glands
Microbiomas
Ovarios
Organismos Patógenos
Glándulas Salivales
Rhipicephalus microplus
topic Anaplasma marginale
Microbiomes
Ovaries
Pathogens
Rhipicephalus
Salivary Glands
Microbiomas
Ovarios
Organismos Patógenos
Glándulas Salivales
Rhipicephalus microplus
dc.description.none.fl_txt_mv The cattle tick Rhipicephalus microplus is a vector for Anaplasma marginale in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study focused on how A. marginale infection affects the bacterial community within the salivary glands and ovaries of R. microplus. Engorged female ticks collected from cattle in a disease-endemic area of Argentina were further classified based on whether they were infected with A. marginale by targeting the msp1β gene through PCR diagnosis in tissue samples. We included negative control samples throughout the study. We analyzed the bacterial communities in tick tissues by sequencing the 16S rRNA V3-V4 region. Our results revealed significant differences in community composition between infected and uninfected samples. Sphingomonadaceae was identified as a predominant taxon in uninfected salivary glands and ovaries. Additionally, we constructed co-occurrence networks to study interactions within the microbial communities. It is noteworthy that A. marginale infection led to an increase in network complexity in the salivary glands, exerting the opposite effect on the ovaries. These findings reinforced the hypothesis that A. marginale impacts the microbiota of R. microplus at an organ-specific level.
Instituto de Biotecnología
Fil: Pérez, Agustina Ericlee. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Pérez, Agustina Ericlee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Abuin-Denis, Lianet. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia
Fil: Abuin-Denis, Lianet. Center for Genetic Engineering and Biotechnology. Animal Biotechnology Department; Cuba
Fil: Piloto-Sardiñas, Elianne. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia
Fil: Piloto-Sardiñas, Elianne. National Center for Animal and Plant Health. Direction of Animal Health; Cuba
Fil: Obregón, Dasiel. University of Guelph. School of Environmental Sciences; Canadá
Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Cabezas-Cruz, Alejandro. Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). ANSES. Unité Mixte de Recherche Biologie et Immunologie Parasitaires (UMR Bipar). Ecole Nationale V´et´erinaire d’Alfort. Laboratoire de Sant´e Animale; Francia
Fil: Farber, Marisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The cattle tick Rhipicephalus microplus is a vector for Anaplasma marginale in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study focused on how A. marginale infection affects the bacterial community within the salivary glands and ovaries of R. microplus. Engorged female ticks collected from cattle in a disease-endemic area of Argentina were further classified based on whether they were infected with A. marginale by targeting the msp1β gene through PCR diagnosis in tissue samples. We included negative control samples throughout the study. We analyzed the bacterial communities in tick tissues by sequencing the 16S rRNA V3-V4 region. Our results revealed significant differences in community composition between infected and uninfected samples. Sphingomonadaceae was identified as a predominant taxon in uninfected salivary glands and ovaries. Additionally, we constructed co-occurrence networks to study interactions within the microbial communities. It is noteworthy that A. marginale infection led to an increase in network complexity in the salivary glands, exerting the opposite effect on the ovaries. These findings reinforced the hypothesis that A. marginale impacts the microbiota of R. microplus at an organ-specific level.
publishDate 2025
dc.date.none.fl_str_mv 2025-09-02T10:08:58Z
2025-09-02T10:08:58Z
2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23644
https://www.sciencedirect.com/science/article/pii/S1877959X2500086X
1877-959X
https://doi.org/10.1016/j.ttbdis.2025.102522
url http://hdl.handle.net/20.500.12123/23644
https://www.sciencedirect.com/science/article/pii/S1877959X2500086X
https://doi.org/10.1016/j.ttbdis.2025.102522
identifier_str_mv 1877-959X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Ticks and Tick-borne Diseases 16 (5) : 102522 (September 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341444889608192
score 13.13397

Publicaciones similares