Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity

Autores
Guizzo, Melina Garcia; Tirloni, Lucas; González, Sergio Alberto; Farber, Marisa Diana; Braz, Glória; Parizi, Luís Fernando; Dedavid e Silva, Lucas Andre; da Silva Vaz, Itabajara; Oliveira, Pedro L.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.
Fil: Guizzo, Melina Garcia. National Institute Of Allergy And Infectious Diseases; Estados Unidos. Universidade Federal do Rio de Janeiro; Brasil
Fil: Tirloni, Lucas. National Institute Of Allergy And Infectious Diseases; Estados Unidos
Fil: González, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Braz, Glória. Universidade Federal do Rio de Janeiro; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Dedavid e Silva, Lucas Andre. Universidade Federal do Rio Grande do Sul; Brasil
Fil: da Silva Vaz, Itabajara. Instituto Nacional de Ciência E Tecnologia Em Entomologia Molecular; Brasil. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Nacional de Ciência E Tecnologia Em Entomologia Molecular; Brasil
Materia
COXIELLA
MICROBIOME
RHIPICEPHALUS MICROPLUS
SYMBIONT
TICK
TRANSCRIPTOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213461
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/213461
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding CapacityGuizzo, Melina GarciaTirloni, LucasGonzález, Sergio AlbertoFarber, Marisa DianaBraz, GlóriaParizi, Luís FernandoDedavid e Silva, Lucas Andreda Silva Vaz, ItabajaraOliveira, Pedro L.COXIELLAMICROBIOMERHIPICEPHALUS MICROPLUSSYMBIONTTICKTRANSCRIPTOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.Fil: Guizzo, Melina Garcia. National Institute Of Allergy And Infectious Diseases; Estados Unidos. Universidade Federal do Rio de Janeiro; BrasilFil: Tirloni, Lucas. National Institute Of Allergy And Infectious Diseases; Estados UnidosFil: González, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Braz, Glória. Universidade Federal do Rio de Janeiro; BrasilFil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul; BrasilFil: Dedavid e Silva, Lucas Andre. Universidade Federal do Rio Grande do Sul; BrasilFil: da Silva Vaz, Itabajara. Instituto Nacional de Ciência E Tecnologia Em Entomologia Molecular; Brasil. Universidade Federal do Rio Grande do Sul; BrasilFil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Nacional de Ciência E Tecnologia Em Entomologia Molecular; BrasilFrontiers Media2022-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213461Guizzo, Melina Garcia; Tirloni, Lucas; González, Sergio Alberto; Farber, Marisa Diana; Braz, Glória; et al.; Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity; Frontiers Media; Frontiers in Microbiology; 13; 5-2022; 1-131664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2022.868575/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2022.868575info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:58Zoai:ri.conicet.gov.ar:11336/213461instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:59.05CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity
title Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity
spellingShingle Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity
Guizzo, Melina Garcia
COXIELLA
MICROBIOME
RHIPICEPHALUS MICROPLUS
SYMBIONT
TICK
TRANSCRIPTOME
title_short Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity
title_full Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity
title_fullStr Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity
title_full_unstemmed Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity
title_sort Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity
dc.creator.none.fl_str_mv Guizzo, Melina Garcia
Tirloni, Lucas
González, Sergio Alberto
Farber, Marisa Diana
Braz, Glória
Parizi, Luís Fernando
Dedavid e Silva, Lucas Andre
da Silva Vaz, Itabajara
Oliveira, Pedro L.
author Guizzo, Melina Garcia
author_facet Guizzo, Melina Garcia
Tirloni, Lucas
González, Sergio Alberto
Farber, Marisa Diana
Braz, Glória
Parizi, Luís Fernando
Dedavid e Silva, Lucas Andre
da Silva Vaz, Itabajara
Oliveira, Pedro L.
author_role author
author2 Tirloni, Lucas
González, Sergio Alberto
Farber, Marisa Diana
Braz, Glória
Parizi, Luís Fernando
Dedavid e Silva, Lucas Andre
da Silva Vaz, Itabajara
Oliveira, Pedro L.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COXIELLA
MICROBIOME
RHIPICEPHALUS MICROPLUS
SYMBIONT
TICK
TRANSCRIPTOME
topic COXIELLA
MICROBIOME
RHIPICEPHALUS MICROPLUS
SYMBIONT
TICK
TRANSCRIPTOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.
Fil: Guizzo, Melina Garcia. National Institute Of Allergy And Infectious Diseases; Estados Unidos. Universidade Federal do Rio de Janeiro; Brasil
Fil: Tirloni, Lucas. National Institute Of Allergy And Infectious Diseases; Estados Unidos
Fil: González, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Braz, Glória. Universidade Federal do Rio de Janeiro; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Dedavid e Silva, Lucas Andre. Universidade Federal do Rio Grande do Sul; Brasil
Fil: da Silva Vaz, Itabajara. Instituto Nacional de Ciência E Tecnologia Em Entomologia Molecular; Brasil. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil. Instituto Nacional de Ciência E Tecnologia Em Entomologia Molecular; Brasil
description In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.
publishDate 2022
dc.date.none.fl_str_mv 2022-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213461
Guizzo, Melina Garcia; Tirloni, Lucas; González, Sergio Alberto; Farber, Marisa Diana; Braz, Glória; et al.; Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity; Frontiers Media; Frontiers in Microbiology; 13; 5-2022; 1-13
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213461
identifier_str_mv Guizzo, Melina Garcia; Tirloni, Lucas; González, Sergio Alberto; Farber, Marisa Diana; Braz, Glória; et al.; Coxiella Endosymbiont of Rhipicephalus microplus Modulates Tick Physiology With a Major Impact in Blood Feeding Capacity; Frontiers Media; Frontiers in Microbiology; 13; 5-2022; 1-13
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2022.868575/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2022.868575
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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