Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus

Autores
Mazzucco Panizza, Matilde Nahimé; Cutulle, Christian; Primo, María Evangelina; Morel, Nicolas; Sebastian, Patrick; Nava, Santiago
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae of R. microplus were fed on a calf infected with the isolate S1P of A. marginale. The engorged females obtained were analyzed by PCR and incubated for oviposition. After hatching, larvae were analyzed by PCR and fed on susceptible splenectomized cattle. Although A. marginale was detected in the females, no A. marginale DNA was amplified from the larvae and transmission of A. marginale to cattle was not recorded. In the second experiment, R. microplus larvae were fed on cattle naturally infected with field isolates of A. marginale and experimentally infected with the isolate S1P of A. marginale. After detachment, engorged females were incubated for oviposition. The offspring were analyzed by PCR, with negative results. Finally, free-living larvae of R. microplus collected from pasture on farms with cattle infected with A. marginale were analyzed by PCR for Anaplasma infection. All samples analyzed were negative for A. marginale. The results of this work indicate that transovarial transmission of A. marginale by R. microplus is unlikely to occur.
EEA Rafaela
Fil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Buenos Aires, Argentina
Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Veterinary Parasitology 311: 109808 (November 2022)
Materia
Anaplasma marginale
Rhipicephalus
Anaplasmosis
Ganado Bovino
Cattle
Transovarial Transmission
Transmisión Transovárica
Rhipicephalus microplus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13684
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13684
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplusMazzucco Panizza, Matilde NahiméCutulle, ChristianPrimo, María EvangelinaMorel, NicolasSebastian, PatrickNava, SantiagoAnaplasma marginaleRhipicephalusAnaplasmosisGanado BovinoCattleTransovarial TransmissionTransmisión TransováricaRhipicephalus microplusThe aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae of R. microplus were fed on a calf infected with the isolate S1P of A. marginale. The engorged females obtained were analyzed by PCR and incubated for oviposition. After hatching, larvae were analyzed by PCR and fed on susceptible splenectomized cattle. Although A. marginale was detected in the females, no A. marginale DNA was amplified from the larvae and transmission of A. marginale to cattle was not recorded. In the second experiment, R. microplus larvae were fed on cattle naturally infected with field isolates of A. marginale and experimentally infected with the isolate S1P of A. marginale. After detachment, engorged females were incubated for oviposition. The offspring were analyzed by PCR, with negative results. Finally, free-living larvae of R. microplus collected from pasture on farms with cattle infected with A. marginale were analyzed by PCR for Anaplasma infection. All samples analyzed were negative for A. marginale. The results of this work indicate that transovarial transmission of A. marginale by R. microplus is unlikely to occur.EEA RafaelaFil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Buenos Aires, ArgentinaFil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaElsevier2022-12-22T11:31:09Z2022-12-22T11:31:09Z2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13684https://www.sciencedirect.com/science/article/abs/pii/S03044017220016250304-4017 (print)1873-2550 (online)https://doi.org/10.1016/j.vetpar.2022.109808Veterinary Parasitology 311: 109808 (November 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y lainfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:40Zoai:localhost:20.500.12123/13684instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:40.875INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
title Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
spellingShingle Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
Mazzucco Panizza, Matilde Nahimé
Anaplasma marginale
Rhipicephalus
Anaplasmosis
Ganado Bovino
Cattle
Transovarial Transmission
Transmisión Transovárica
Rhipicephalus microplus
title_short Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
title_full Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
title_fullStr Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
title_full_unstemmed Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
title_sort Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
dc.creator.none.fl_str_mv Mazzucco Panizza, Matilde Nahimé
Cutulle, Christian
Primo, María Evangelina
Morel, Nicolas
Sebastian, Patrick
Nava, Santiago
author Mazzucco Panizza, Matilde Nahimé
author_facet Mazzucco Panizza, Matilde Nahimé
Cutulle, Christian
Primo, María Evangelina
Morel, Nicolas
Sebastian, Patrick
Nava, Santiago
author_role author
author2 Cutulle, Christian
Primo, María Evangelina
Morel, Nicolas
Sebastian, Patrick
Nava, Santiago
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Anaplasma marginale
Rhipicephalus
Anaplasmosis
Ganado Bovino
Cattle
Transovarial Transmission
Transmisión Transovárica
Rhipicephalus microplus
topic Anaplasma marginale
Rhipicephalus
Anaplasmosis
Ganado Bovino
Cattle
Transovarial Transmission
Transmisión Transovárica
Rhipicephalus microplus
dc.description.none.fl_txt_mv The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae of R. microplus were fed on a calf infected with the isolate S1P of A. marginale. The engorged females obtained were analyzed by PCR and incubated for oviposition. After hatching, larvae were analyzed by PCR and fed on susceptible splenectomized cattle. Although A. marginale was detected in the females, no A. marginale DNA was amplified from the larvae and transmission of A. marginale to cattle was not recorded. In the second experiment, R. microplus larvae were fed on cattle naturally infected with field isolates of A. marginale and experimentally infected with the isolate S1P of A. marginale. After detachment, engorged females were incubated for oviposition. The offspring were analyzed by PCR, with negative results. Finally, free-living larvae of R. microplus collected from pasture on farms with cattle infected with A. marginale were analyzed by PCR for Anaplasma infection. All samples analyzed were negative for A. marginale. The results of this work indicate that transovarial transmission of A. marginale by R. microplus is unlikely to occur.
EEA Rafaela
Fil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Mazzucco Panizza, Matilde Nahimé. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Cutulle, Christian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Buenos Aires, Argentina
Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae of R. microplus were fed on a calf infected with the isolate S1P of A. marginale. The engorged females obtained were analyzed by PCR and incubated for oviposition. After hatching, larvae were analyzed by PCR and fed on susceptible splenectomized cattle. Although A. marginale was detected in the females, no A. marginale DNA was amplified from the larvae and transmission of A. marginale to cattle was not recorded. In the second experiment, R. microplus larvae were fed on cattle naturally infected with field isolates of A. marginale and experimentally infected with the isolate S1P of A. marginale. After detachment, engorged females were incubated for oviposition. The offspring were analyzed by PCR, with negative results. Finally, free-living larvae of R. microplus collected from pasture on farms with cattle infected with A. marginale were analyzed by PCR for Anaplasma infection. All samples analyzed were negative for A. marginale. The results of this work indicate that transovarial transmission of A. marginale by R. microplus is unlikely to occur.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-22T11:31:09Z
2022-12-22T11:31:09Z
2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13684
https://www.sciencedirect.com/science/article/abs/pii/S0304401722001625
0304-4017 (print)
1873-2550 (online)
https://doi.org/10.1016/j.vetpar.2022.109808
url http://hdl.handle.net/20.500.12123/13684
https://www.sciencedirect.com/science/article/abs/pii/S0304401722001625
https://doi.org/10.1016/j.vetpar.2022.109808
identifier_str_mv 0304-4017 (print)
1873-2550 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology 311: 109808 (November 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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