Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity

Autores
De La Fourniere, Sofia; Guillemi, Eliana Carolina; Paoletta, Martina; Pérez, Agustina Ericlee; Obregón, Dasiel; Cabezas-Cruz, Alejandro; Sarmiento, Nestor Fabian; Farber, Marisa Diana
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anaplasma marginale is an obligate intraerythrocytic bacterium of bovines, responsible for large economic losses worldwide. It is mainly transmitted by Rhipicephalus (Boophilus) microplus ticks and, despite mounting evidence suggesting transovarial transmission, the occurrence of this phenomenon remains controversial. We evaluated the vector competence of R. microplus larvae vertically infected with A. marginale to transmit the bacterium to a naïve bovine. A subgroup of engorged female ticks collected from an A. marginale-positive animal was dissected and the presence of the pathogen in its tissues was confirmed. A second subgroup of ticks was placed under controlled conditions for oviposition. After confirming the presence of A. marginale in the hatched larvae, an experimental infestation assay was conducted. Larvae were placed on an A. marginale-free splenectomized calf. The bacterium was detected in the experimentally infested bovine 22 days post-infestation. We analyzed the A. marginale diversity throughout the transmission cycle using the molecular marker MSP1a. Different genotypes were detected in the mammalian and arthropod hosts showing a reduction of strain diversity along the transmission process. Our results demonstrate the vertical transmission of A. marginale from R. microplus females to its larvae, their vector competence to transmit the pathogen, and a bottleneck in A. marginale strain diversity.
Instituto de Biotecnología
Fil: De La Fourniere, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: De La Fourniere, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Paoletta, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, Agustina Ericlee. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Pérez, Agustina Ericlee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Obregón, Dasiel. University of Guelph. School of Environmental Sciences; Canadá
Fil: Cabezas-Cruz, Alejandro. Agence Nationale de Sécurité Sanitaire de l’Alimentation (ANSES). Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). Ecole Nationale Vétérinarie d’Alfort. Laboratoire de Santé Animale; Francia
Fil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Pathogens 12 (8) : 1010 (Agosto 2023)
Materia
Anaplasma marginale
Rhipicephalus
Vectors
Vectores
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15302
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15302
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversityDe La Fourniere, SofiaGuillemi, Eliana CarolinaPaoletta, MartinaPérez, Agustina EricleeObregón, DasielCabezas-Cruz, AlejandroSarmiento, Nestor FabianFarber, Marisa DianaAnaplasma marginaleRhipicephalusVectorsVectoresAnaplasma marginale is an obligate intraerythrocytic bacterium of bovines, responsible for large economic losses worldwide. It is mainly transmitted by Rhipicephalus (Boophilus) microplus ticks and, despite mounting evidence suggesting transovarial transmission, the occurrence of this phenomenon remains controversial. We evaluated the vector competence of R. microplus larvae vertically infected with A. marginale to transmit the bacterium to a naïve bovine. A subgroup of engorged female ticks collected from an A. marginale-positive animal was dissected and the presence of the pathogen in its tissues was confirmed. A second subgroup of ticks was placed under controlled conditions for oviposition. After confirming the presence of A. marginale in the hatched larvae, an experimental infestation assay was conducted. Larvae were placed on an A. marginale-free splenectomized calf. The bacterium was detected in the experimentally infested bovine 22 days post-infestation. We analyzed the A. marginale diversity throughout the transmission cycle using the molecular marker MSP1a. Different genotypes were detected in the mammalian and arthropod hosts showing a reduction of strain diversity along the transmission process. Our results demonstrate the vertical transmission of A. marginale from R. microplus females to its larvae, their vector competence to transmit the pathogen, and a bottleneck in A. marginale strain diversity.Instituto de BiotecnologíaFil: De La Fourniere, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: De La Fourniere, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Paoletta, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez, Agustina Ericlee. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Pérez, Agustina Ericlee. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Obregón, Dasiel. University of Guelph. School of Environmental Sciences; CanadáFil: Cabezas-Cruz, Alejandro. Agence Nationale de Sécurité Sanitaire de l’Alimentation (ANSES). Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). Ecole Nationale Vétérinarie d’Alfort. Laboratoire de Santé Animale; FranciaFil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2023-09-25T14:42:05Z2023-09-25T14:42:05Z2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15302https://www.mdpi.com/2076-0817/12/8/10102076-0817https://doi.org/10.3390/pathogens12081010Pathogens 12 (8) : 1010 (Agosto 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:58Zoai:localhost:20.500.12123/15302instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:58.307INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
title Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
spellingShingle Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
De La Fourniere, Sofia
Anaplasma marginale
Rhipicephalus
Vectors
Vectores
title_short Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
title_full Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
title_fullStr Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
title_full_unstemmed Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
title_sort Transovarial transmission of anaplasma marginale in Rhipicephalus (Boophilus) microplus ticks results in a bottleneck for strain diversity
dc.creator.none.fl_str_mv De La Fourniere, Sofia
Guillemi, Eliana Carolina
Paoletta, Martina
Pérez, Agustina Ericlee
Obregón, Dasiel
Cabezas-Cruz, Alejandro
Sarmiento, Nestor Fabian
Farber, Marisa Diana
author De La Fourniere, Sofia
author_facet De La Fourniere, Sofia
Guillemi, Eliana Carolina
Paoletta, Martina
Pérez, Agustina Ericlee
Obregón, Dasiel
Cabezas-Cruz, Alejandro
Sarmiento, Nestor Fabian
Farber, Marisa Diana
author_role author
author2 Guillemi, Eliana Carolina
Paoletta, Martina
Pérez, Agustina Ericlee
Obregón, Dasiel
Cabezas-Cruz, Alejandro
Sarmiento, Nestor Fabian
Farber, Marisa Diana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anaplasma marginale
Rhipicephalus
Vectors
Vectores
topic Anaplasma marginale
Rhipicephalus
Vectors
Vectores
dc.description.none.fl_txt_mv Anaplasma marginale is an obligate intraerythrocytic bacterium of bovines, responsible for large economic losses worldwide. It is mainly transmitted by Rhipicephalus (Boophilus) microplus ticks and, despite mounting evidence suggesting transovarial transmission, the occurrence of this phenomenon remains controversial. We evaluated the vector competence of R. microplus larvae vertically infected with A. marginale to transmit the bacterium to a naïve bovine. A subgroup of engorged female ticks collected from an A. marginale-positive animal was dissected and the presence of the pathogen in its tissues was confirmed. A second subgroup of ticks was placed under controlled conditions for oviposition. After confirming the presence of A. marginale in the hatched larvae, an experimental infestation assay was conducted. Larvae were placed on an A. marginale-free splenectomized calf. The bacterium was detected in the experimentally infested bovine 22 days post-infestation. We analyzed the A. marginale diversity throughout the transmission cycle using the molecular marker MSP1a. Different genotypes were detected in the mammalian and arthropod hosts showing a reduction of strain diversity along the transmission process. Our results demonstrate the vertical transmission of A. marginale from R. microplus females to its larvae, their vector competence to transmit the pathogen, and a bottleneck in A. marginale strain diversity.
Instituto de Biotecnología
Fil: De La Fourniere, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: De La Fourniere, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Guillemi, Eliana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Paoletta, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez, Agustina Ericlee. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Pérez, Agustina Ericlee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Obregón, Dasiel. University of Guelph. School of Environmental Sciences; Canadá
Fil: Cabezas-Cruz, Alejandro. Agence Nationale de Sécurité Sanitaire de l’Alimentation (ANSES). Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE). Ecole Nationale Vétérinarie d’Alfort. Laboratoire de Santé Animale; Francia
Fil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Anaplasma marginale is an obligate intraerythrocytic bacterium of bovines, responsible for large economic losses worldwide. It is mainly transmitted by Rhipicephalus (Boophilus) microplus ticks and, despite mounting evidence suggesting transovarial transmission, the occurrence of this phenomenon remains controversial. We evaluated the vector competence of R. microplus larvae vertically infected with A. marginale to transmit the bacterium to a naïve bovine. A subgroup of engorged female ticks collected from an A. marginale-positive animal was dissected and the presence of the pathogen in its tissues was confirmed. A second subgroup of ticks was placed under controlled conditions for oviposition. After confirming the presence of A. marginale in the hatched larvae, an experimental infestation assay was conducted. Larvae were placed on an A. marginale-free splenectomized calf. The bacterium was detected in the experimentally infested bovine 22 days post-infestation. We analyzed the A. marginale diversity throughout the transmission cycle using the molecular marker MSP1a. Different genotypes were detected in the mammalian and arthropod hosts showing a reduction of strain diversity along the transmission process. Our results demonstrate the vertical transmission of A. marginale from R. microplus females to its larvae, their vector competence to transmit the pathogen, and a bottleneck in A. marginale strain diversity.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-25T14:42:05Z
2023-09-25T14:42:05Z
2023-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15302
https://www.mdpi.com/2076-0817/12/8/1010
2076-0817
https://doi.org/10.3390/pathogens12081010
url http://hdl.handle.net/20.500.12123/15302
https://www.mdpi.com/2076-0817/12/8/1010
https://doi.org/10.3390/pathogens12081010
identifier_str_mv 2076-0817
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Pathogens 12 (8) : 1010 (Agosto 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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