Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity

Autores
Garcia Guizzo, Melina; Tirloni, Lucas; Gonzalez, Sergio Alberto; Farber, Marisa Diana; Braz, Glória; Parizi, Luís Fernando; Dedavid e Silva, Lucas Andre; Da Silva Vaz, Itabajara; Oliveira, Pedro L.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.
Instituto de Biotecnología
Fil: Garcia Guizzo, Melina. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Biology Section; Estados Unidos
Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Tirloni, Lucas. National Institute of Allergy and Infectious Diseases. Rocky Mountain Laboratories. Laboratory of Bacteriology. Tick-Pathogen Transmission Unit; Estados Unidos
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnologia y Biologia Molecular; Argentina
Fil: Gonzalez, Sergio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnologia y Biologia Molecular; Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Braz, Glória. Universidade Federal do Rio de Janeiro. Instituto de Química; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Dedavid e Silva, Lucas Andre. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária; Brasil
Fil: Da Silva Vaz, Itabajara. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Fil: Oliveira, Pedro L. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Oliveira, Pedro L. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Fuente
Frontiers in Microbiology 13 : 868575 (Mayo 2022)
Materia
Coxiella
Simbiontico
Transcriptomas
Microbiomas
Sanidad Animal
Symbionts
Rhipicephalus
Transcriptome
Microbiomes
Animal Health
Rhipicephalus microplus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12156
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oai_identifier_str oai:localhost:20.500.12123/12156
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacityGarcia Guizzo, MelinaTirloni, LucasGonzalez, Sergio AlbertoFarber, Marisa DianaBraz, GlóriaParizi, Luís FernandoDedavid e Silva, Lucas AndreDa Silva Vaz, ItabajaraOliveira, Pedro L.CoxiellaSimbionticoTranscriptomasMicrobiomasSanidad AnimalSymbiontsRhipicephalusTranscriptomeMicrobiomesAnimal HealthRhipicephalus microplusIn the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.Instituto de BiotecnologíaFil: Garcia Guizzo, Melina. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Biology Section; Estados UnidosFil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; BrasilFil: Tirloni, Lucas. National Institute of Allergy and Infectious Diseases. Rocky Mountain Laboratories. Laboratory of Bacteriology. Tick-Pathogen Transmission Unit; Estados UnidosFil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnologia y Biologia Molecular; ArgentinaFil: Gonzalez, Sergio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnologia y Biologia Molecular; ArgentinaFil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Braz, Glória. Universidade Federal do Rio de Janeiro. Instituto de Química; BrasilFil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; BrasilFil: Dedavid e Silva, Lucas Andre. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; BrasilFil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; BrasilFil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária; BrasilFil: Da Silva Vaz, Itabajara. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; BrasilFil: Oliveira, Pedro L. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; BrasilFil: Oliveira, Pedro L. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; BrasilFrontiers Media2022-06-24T10:31:46Z2022-06-24T10:31:46Z2022-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12156https://www.frontiersin.org/articles/10.3389/fmicb.2022.868575/full1664-302Xhttps://doi.org/10.3389/fmicb.2022.868575Frontiers in Microbiology 13 : 868575 (Mayo 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131043/AR./Bioinformática y Estadística Genómica.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:30:47Zoai:localhost:20.500.12123/12156instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:47.701INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
title Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
spellingShingle Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
Garcia Guizzo, Melina
Coxiella
Simbiontico
Transcriptomas
Microbiomas
Sanidad Animal
Symbionts
Rhipicephalus
Transcriptome
Microbiomes
Animal Health
Rhipicephalus microplus
title_short Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
title_full Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
title_fullStr Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
title_full_unstemmed Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
title_sort Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
dc.creator.none.fl_str_mv Garcia Guizzo, Melina
Tirloni, Lucas
Gonzalez, Sergio Alberto
Farber, Marisa Diana
Braz, Glória
Parizi, Luís Fernando
Dedavid e Silva, Lucas Andre
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author Garcia Guizzo, Melina
author_facet Garcia Guizzo, Melina
Tirloni, Lucas
Gonzalez, Sergio Alberto
Farber, Marisa Diana
Braz, Glória
Parizi, Luís Fernando
Dedavid e Silva, Lucas Andre
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author_role author
author2 Tirloni, Lucas
Gonzalez, Sergio Alberto
Farber, Marisa Diana
Braz, Glória
Parizi, Luís Fernando
Dedavid e Silva, Lucas Andre
Da Silva Vaz, Itabajara
Oliveira, Pedro L.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Coxiella
Simbiontico
Transcriptomas
Microbiomas
Sanidad Animal
Symbionts
Rhipicephalus
Transcriptome
Microbiomes
Animal Health
Rhipicephalus microplus
topic Coxiella
Simbiontico
Transcriptomas
Microbiomas
Sanidad Animal
Symbionts
Rhipicephalus
Transcriptome
Microbiomes
Animal Health
Rhipicephalus microplus
dc.description.none.fl_txt_mv In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.
Instituto de Biotecnología
Fil: Garcia Guizzo, Melina. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Biology Section; Estados Unidos
Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Tirloni, Lucas. National Institute of Allergy and Infectious Diseases. Rocky Mountain Laboratories. Laboratory of Bacteriology. Tick-Pathogen Transmission Unit; Estados Unidos
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnologia y Biologia Molecular; Argentina
Fil: Gonzalez, Sergio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnologia y Biologia Molecular; Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Braz, Glória. Universidade Federal do Rio de Janeiro. Instituto de Química; Brasil
Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Dedavid e Silva, Lucas Andre. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil
Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária; Brasil
Fil: Da Silva Vaz, Itabajara. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
Fil: Oliveira, Pedro L. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil
Fil: Oliveira, Pedro L. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil
description In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction.
publishDate 2022
dc.date.none.fl_str_mv 2022-06-24T10:31:46Z
2022-06-24T10:31:46Z
2022-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12156
https://www.frontiersin.org/articles/10.3389/fmicb.2022.868575/full
1664-302X
https://doi.org/10.3389/fmicb.2022.868575
url http://hdl.handle.net/20.500.12123/12156
https://www.frontiersin.org/articles/10.3389/fmicb.2022.868575/full
https://doi.org/10.3389/fmicb.2022.868575
identifier_str_mv 1664-302X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO-1131043/AR./Bioinformática y Estadística Genómica.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Microbiology 13 : 868575 (Mayo 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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