Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)

Autores
Bejerman, Nicolas Esteban; De Breuil, Soledad; Debat, Humberto Julio; Miretti, Marcos; Badaracco, Alejandra; Nome Docampo, Claudia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in sequence and genome structure. We have determined the complete genome sequence of this virus, which is 12,876 nt long. Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-P4-M-G-L-5′. Phylogenetic analysis suggested that the described virus is a new member of the genus Cytorhabdovirus, which was supported by the observation of rhabdovirus-like particles within the cytoplasm of infected yerba mate cells. The virus has been tentatively named “yerba mate chlorosis-associated virus” (YmCaV). The availability of the YmCaV genome sequence will contribute to assessing the genetic variability of this virus and determining its role in this yerba mate disease.
Inst. Patología Vegetal
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Debat, Humberto Julio Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.
Fil: Miretti, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Badaracco, Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Archives of virology 162 (8) : 2481–2484. (August 2017)
Materia
Ilex Paraguariensis
Virus de las Plantas
Clorosis
Genética Molecular
Plant Viruses
Chlorosis
Molecular Genetics
Yerba Mate
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1453
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1453
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spelling Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)Bejerman, Nicolas EstebanDe Breuil, SoledadDebat, Humberto JulioMiretti, MarcosBadaracco, AlejandraNome Docampo, ClaudiaIlex ParaguariensisVirus de las PlantasClorosisGenética MolecularPlant VirusesChlorosisMolecular GeneticsYerba MateWe present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in sequence and genome structure. We have determined the complete genome sequence of this virus, which is 12,876 nt long. Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-P4-M-G-L-5′. Phylogenetic analysis suggested that the described virus is a new member of the genus Cytorhabdovirus, which was supported by the observation of rhabdovirus-like particles within the cytoplasm of infected yerba mate cells. The virus has been tentatively named “yerba mate chlorosis-associated virus” (YmCaV). The availability of the YmCaV genome sequence will contribute to assessing the genetic variability of this virus and determining its role in this yerba mate disease.Inst. Patología VegetalFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Debat, Humberto Julio Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.Fil: Miretti, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Badaracco, Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaNome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina2017-10-11T11:43:09Z2017-10-11T11:43:09Z2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1453https://link.springer.com/article/10.1007%2Fs00705-017-3363-80304-8608 (Print)1432-8798 (Online)https://doi.org/10.1007/s00705-017-3363-8Archives of virology 162 (8) : 2481–2484. (August 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:05Zoai:localhost:20.500.12123/1453instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:06.314INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
title Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
spellingShingle Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
Bejerman, Nicolas Esteban
Ilex Paraguariensis
Virus de las Plantas
Clorosis
Genética Molecular
Plant Viruses
Chlorosis
Molecular Genetics
Yerba Mate
title_short Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
title_full Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
title_fullStr Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
title_full_unstemmed Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
title_sort Molecular characterization of yerba mate chlorosis-associated virus, a putative cytorhabdvirus infecting yerba mate (Ilex paraguariensis)
dc.creator.none.fl_str_mv Bejerman, Nicolas Esteban
De Breuil, Soledad
Debat, Humberto Julio
Miretti, Marcos
Badaracco, Alejandra
Nome Docampo, Claudia
author Bejerman, Nicolas Esteban
author_facet Bejerman, Nicolas Esteban
De Breuil, Soledad
Debat, Humberto Julio
Miretti, Marcos
Badaracco, Alejandra
Nome Docampo, Claudia
author_role author
author2 De Breuil, Soledad
Debat, Humberto Julio
Miretti, Marcos
Badaracco, Alejandra
Nome Docampo, Claudia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ilex Paraguariensis
Virus de las Plantas
Clorosis
Genética Molecular
Plant Viruses
Chlorosis
Molecular Genetics
Yerba Mate
topic Ilex Paraguariensis
Virus de las Plantas
Clorosis
Genética Molecular
Plant Viruses
Chlorosis
Molecular Genetics
Yerba Mate
dc.description.none.fl_txt_mv We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in sequence and genome structure. We have determined the complete genome sequence of this virus, which is 12,876 nt long. Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-P4-M-G-L-5′. Phylogenetic analysis suggested that the described virus is a new member of the genus Cytorhabdovirus, which was supported by the observation of rhabdovirus-like particles within the cytoplasm of infected yerba mate cells. The virus has been tentatively named “yerba mate chlorosis-associated virus” (YmCaV). The availability of the YmCaV genome sequence will contribute to assessing the genetic variability of this virus and determining its role in this yerba mate disease.
Inst. Patología Vegetal
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Debat, Humberto Julio Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina.
Fil: Miretti, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Badaracco, Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description We present the molecular characterization of a new virus infecting yerba mate (Ilex paraguariensis St. Hil.) in Argentina. Deep sequencing of diseased yerba mate plants showing chlorotic linear patterns, chlorotic rings, and vein yellowing resulted in the identification of a new virus resembling plant rhabdoviruses in sequence and genome structure. We have determined the complete genome sequence of this virus, which is 12,876 nt long. Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-P4-M-G-L-5′. Phylogenetic analysis suggested that the described virus is a new member of the genus Cytorhabdovirus, which was supported by the observation of rhabdovirus-like particles within the cytoplasm of infected yerba mate cells. The virus has been tentatively named “yerba mate chlorosis-associated virus” (YmCaV). The availability of the YmCaV genome sequence will contribute to assessing the genetic variability of this virus and determining its role in this yerba mate disease.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-11T11:43:09Z
2017-10-11T11:43:09Z
2017-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1453
https://link.springer.com/article/10.1007%2Fs00705-017-3363-8
0304-8608 (Print)
1432-8798 (Online)
https://doi.org/10.1007/s00705-017-3363-8
url http://hdl.handle.net/20.500.12123/1453
https://link.springer.com/article/10.1007%2Fs00705-017-3363-8
https://doi.org/10.1007/s00705-017-3363-8
identifier_str_mv 0304-8608 (Print)
1432-8798 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Archives of virology 162 (8) : 2481–2484. (August 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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