Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina

Autores
Ruiz Posse, Agustina María; Camiletti, Boris Xavier; Gimenez, Maria De La Paz; Torrico Ramallo, Ada Karina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Non-aflatoxigenic Aspergillus flavus strains have emerged as promising biocontrol agents to reduce aflatoxin contamination in maize crops. This study aimed to evaluate the growth dynamics, sporulation capacity and phenotypic stability of local non-aflatoxigenic A. flavus strains from Argentina compared to aflatoxigenic strains. Eighteen non-aflatoxigenic strains, isolated from maize ears grown in Argentina's central agricultural region, were assessed for growth and spore production at various temperatures, with the results indicating superior growth and sporulation under optimal conditions (30°C). The phenotypic stability of these strains was tested by conducting 20 consecutive subcultures, confirming the retention of their non-aflatoxigenic character. The local non-aflatoxigenic strains lack the nucleotide 591 polymorphism in the pksA gene, indicating that their inability to produce aflatoxins is not due to this mutation. These strains demonstrated the ability to survive and grow under high-temperature conditions, making them suitable candidates for biocontrol applications in regions with variable climates and significant thermal amplitudes. The findings support the potential of these local non-aflatoxigenic strains as effective biocontrol agents to mitigate aflatoxin contamination in maize production systems in Argentina.
Instituto de Patología Vegetal
Fil: Ruiz Posse, Agustina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Ruiz Posse, Agustina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Camiletti, Boris Xavier. University of Illinois Urbana-Champaign. Department of Crop Sciences; USA
Fil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Torrico Ramallo, Ada Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Plant pathology : 1-10. (First published: 31 July 2025)
Materia
Biological Control
Aflatoxins
Maize
Control Biológico
Argentina
Aspergillus flavus
Aflatoxinas
Maíz
Biocontrol
Growth Curve
Phenotypic Characterisation
Sporulation Curve
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in ArgentinaRuiz Posse, Agustina MaríaCamiletti, Boris XavierGimenez, Maria De La PazTorrico Ramallo, Ada KarinaBiological ControlAflatoxinsMaizeControl BiológicoArgentinaAspergillus flavusAflatoxinasMaízBiocontrolGrowth CurvePhenotypic CharacterisationSporulation CurveNon-aflatoxigenic Aspergillus flavus strains have emerged as promising biocontrol agents to reduce aflatoxin contamination in maize crops. This study aimed to evaluate the growth dynamics, sporulation capacity and phenotypic stability of local non-aflatoxigenic A. flavus strains from Argentina compared to aflatoxigenic strains. Eighteen non-aflatoxigenic strains, isolated from maize ears grown in Argentina's central agricultural region, were assessed for growth and spore production at various temperatures, with the results indicating superior growth and sporulation under optimal conditions (30°C). The phenotypic stability of these strains was tested by conducting 20 consecutive subcultures, confirming the retention of their non-aflatoxigenic character. The local non-aflatoxigenic strains lack the nucleotide 591 polymorphism in the pksA gene, indicating that their inability to produce aflatoxins is not due to this mutation. These strains demonstrated the ability to survive and grow under high-temperature conditions, making them suitable candidates for biocontrol applications in regions with variable climates and significant thermal amplitudes. The findings support the potential of these local non-aflatoxigenic strains as effective biocontrol agents to mitigate aflatoxin contamination in maize production systems in Argentina.Instituto de Patología VegetalFil: Ruiz Posse, Agustina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Ruiz Posse, Agustina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Camiletti, Boris Xavier. University of Illinois Urbana-Champaign. Department of Crop Sciences; USAFil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Torrico Ramallo, Ada Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaWiley2025-08-29T16:12:06Z2025-08-29T16:12:06Z2025-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23627https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.700300032-08621365-3059https://doi.org/10.1111/ppa.70030Plant pathology : 1-10. (First published: 31 July 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestalesinfo:eu-repograntAgreement/INTA/2023-PE-L04-I120, Inocuidad física, química y biológica de los alimentos. Metodologías analíticas, tecnologías, estrategias de intervención innovadoras y análisis de riesgoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:16Zoai:localhost:20.500.12123/23627instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:16.534INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina
title Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina
spellingShingle Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina
Ruiz Posse, Agustina María
Biological Control
Aflatoxins
Maize
Control Biológico
Argentina
Aspergillus flavus
Aflatoxinas
Maíz
Biocontrol
Growth Curve
Phenotypic Characterisation
Sporulation Curve
title_short Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina
title_full Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina
title_fullStr Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina
title_full_unstemmed Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina
title_sort Growth Dynamics, Sporulation and Stability of Aflatoxin Production in Aspergillus flavus Strains From Preharvest Maize in Argentina
dc.creator.none.fl_str_mv Ruiz Posse, Agustina María
Camiletti, Boris Xavier
Gimenez, Maria De La Paz
Torrico Ramallo, Ada Karina
author Ruiz Posse, Agustina María
author_facet Ruiz Posse, Agustina María
Camiletti, Boris Xavier
Gimenez, Maria De La Paz
Torrico Ramallo, Ada Karina
author_role author
author2 Camiletti, Boris Xavier
Gimenez, Maria De La Paz
Torrico Ramallo, Ada Karina
author2_role author
author
author
dc.subject.none.fl_str_mv Biological Control
Aflatoxins
Maize
Control Biológico
Argentina
Aspergillus flavus
Aflatoxinas
Maíz
Biocontrol
Growth Curve
Phenotypic Characterisation
Sporulation Curve
topic Biological Control
Aflatoxins
Maize
Control Biológico
Argentina
Aspergillus flavus
Aflatoxinas
Maíz
Biocontrol
Growth Curve
Phenotypic Characterisation
Sporulation Curve
dc.description.none.fl_txt_mv Non-aflatoxigenic Aspergillus flavus strains have emerged as promising biocontrol agents to reduce aflatoxin contamination in maize crops. This study aimed to evaluate the growth dynamics, sporulation capacity and phenotypic stability of local non-aflatoxigenic A. flavus strains from Argentina compared to aflatoxigenic strains. Eighteen non-aflatoxigenic strains, isolated from maize ears grown in Argentina's central agricultural region, were assessed for growth and spore production at various temperatures, with the results indicating superior growth and sporulation under optimal conditions (30°C). The phenotypic stability of these strains was tested by conducting 20 consecutive subcultures, confirming the retention of their non-aflatoxigenic character. The local non-aflatoxigenic strains lack the nucleotide 591 polymorphism in the pksA gene, indicating that their inability to produce aflatoxins is not due to this mutation. These strains demonstrated the ability to survive and grow under high-temperature conditions, making them suitable candidates for biocontrol applications in regions with variable climates and significant thermal amplitudes. The findings support the potential of these local non-aflatoxigenic strains as effective biocontrol agents to mitigate aflatoxin contamination in maize production systems in Argentina.
Instituto de Patología Vegetal
Fil: Ruiz Posse, Agustina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Ruiz Posse, Agustina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Camiletti, Boris Xavier. University of Illinois Urbana-Champaign. Department of Crop Sciences; USA
Fil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Torrico Ramallo, Ada Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Non-aflatoxigenic Aspergillus flavus strains have emerged as promising biocontrol agents to reduce aflatoxin contamination in maize crops. This study aimed to evaluate the growth dynamics, sporulation capacity and phenotypic stability of local non-aflatoxigenic A. flavus strains from Argentina compared to aflatoxigenic strains. Eighteen non-aflatoxigenic strains, isolated from maize ears grown in Argentina's central agricultural region, were assessed for growth and spore production at various temperatures, with the results indicating superior growth and sporulation under optimal conditions (30°C). The phenotypic stability of these strains was tested by conducting 20 consecutive subcultures, confirming the retention of their non-aflatoxigenic character. The local non-aflatoxigenic strains lack the nucleotide 591 polymorphism in the pksA gene, indicating that their inability to produce aflatoxins is not due to this mutation. These strains demonstrated the ability to survive and grow under high-temperature conditions, making them suitable candidates for biocontrol applications in regions with variable climates and significant thermal amplitudes. The findings support the potential of these local non-aflatoxigenic strains as effective biocontrol agents to mitigate aflatoxin contamination in maize production systems in Argentina.
publishDate 2025
dc.date.none.fl_str_mv 2025-08-29T16:12:06Z
2025-08-29T16:12:06Z
2025-07-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23627
https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.70030
0032-0862
1365-3059
https://doi.org/10.1111/ppa.70030
url http://hdl.handle.net/20.500.12123/23627
https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.70030
https://doi.org/10.1111/ppa.70030
identifier_str_mv 0032-0862
1365-3059
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L04-I073, Desarrollo de bioinsumos y su integración en estrategias de manejo de adversidades bióticas y abióticas en cultivos agrícolas y forestales
info:eu-repograntAgreement/INTA/2023-PE-L04-I120, Inocuidad física, química y biológica de los alimentos. Metodologías analíticas, tecnologías, estrategias de intervención innovadoras y análisis de riesgo
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant pathology : 1-10. (First published: 31 July 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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