Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum

Autores
Pontin, Mariela Ana; Bottini, Ambrosio Rubén; Burba, José Luis; Piccoli, Patricia Noemí
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro-grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limonene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene might be disruption of fungal membrane integrity.
Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
Hyphal Morphology
Garlic
Allium Sativum
Amaryllidaceae
Sclerotium Cepivorum
White Rot Disease
Terpenes
Antifungal Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22405
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22405
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorumPontin, Mariela AnaBottini, Ambrosio RubénBurba, José LuisPiccoli, Patricia NoemíHyphal MorphologyGarlicAllium SativumAmaryllidaceaeSclerotium CepivorumWhite Rot DiseaseTerpenesAntifungal ActivityThis study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro-grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limonene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene might be disruption of fungal membrane integrity.Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaElsevier2015-07-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22405Pontin, Mariela Ana; Bottini, Ambrosio Rubén; Burba, José Luis; Piccoli, Patricia Noemí; Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum; Elsevier; Phytochemistry; 115; 26-7-2015; 152-1600031-9422CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0031942215000503info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2015.02.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:29:47Zoai:ri.conicet.gov.ar:11336/22405instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:29:48.098CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
spellingShingle Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
Pontin, Mariela Ana
Hyphal Morphology
Garlic
Allium Sativum
Amaryllidaceae
Sclerotium Cepivorum
White Rot Disease
Terpenes
Antifungal Activity
title_short Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title_full Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title_fullStr Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title_full_unstemmed Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
title_sort Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum
dc.creator.none.fl_str_mv Pontin, Mariela Ana
Bottini, Ambrosio Rubén
Burba, José Luis
Piccoli, Patricia Noemí
author Pontin, Mariela Ana
author_facet Pontin, Mariela Ana
Bottini, Ambrosio Rubén
Burba, José Luis
Piccoli, Patricia Noemí
author_role author
author2 Bottini, Ambrosio Rubén
Burba, José Luis
Piccoli, Patricia Noemí
author2_role author
author
author
dc.subject.none.fl_str_mv Hyphal Morphology
Garlic
Allium Sativum
Amaryllidaceae
Sclerotium Cepivorum
White Rot Disease
Terpenes
Antifungal Activity
topic Hyphal Morphology
Garlic
Allium Sativum
Amaryllidaceae
Sclerotium Cepivorum
White Rot Disease
Terpenes
Antifungal Activity
dc.description.none.fl_txt_mv This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro-grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limonene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene might be disruption of fungal membrane integrity.
Fil: Pontin, Mariela Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro-grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limonene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene might be disruption of fungal membrane integrity.
publishDate 2015
dc.date.none.fl_str_mv 2015-07-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22405
Pontin, Mariela Ana; Bottini, Ambrosio Rubén; Burba, José Luis; Piccoli, Patricia Noemí; Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum; Elsevier; Phytochemistry; 115; 26-7-2015; 152-160
0031-9422
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22405
identifier_str_mv Pontin, Mariela Ana; Bottini, Ambrosio Rubén; Burba, José Luis; Piccoli, Patricia Noemí; Allium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum; Elsevier; Phytochemistry; 115; 26-7-2015; 152-160
0031-9422
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0031942215000503
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phytochem.2015.02.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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