Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs

Autores
Hill, Holly; Reddick, David; Caspe, Sergio Gaston; Ramage, Clifford; Frew, David; Rocchi, Mara S.; Opriessnig, Tanja; McNeilly, Tom Nathan
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Atypical porcine pestivirus (APPV) is a novel member of the Pestivirus genus detected in association with congenital tremor (CT) type A-II outbreaks and from apparently healthy pigs, both as singular infection and as part of multi-pathogen infections. 'Classical' pestiviruses are known to cause immunosuppression of their host, which can increase susceptibility to secondary infections, severely impacting health, welfare, and production. To investigate APPV's effect on the host's immune system and characterise disease outcomes, 12 piglets from a natural APPV CT type A-II outbreak were experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV), a significant porcine pathogen. Rectal temperatures indicating febrile responses, viremia and viral-specific humoral and cellular responses were assessed throughout the study. Pathological assessment of the lungs and APPV-PRRSV co-localisation within the lungs was performed at necropsy. Viral co-localisation and pathological assessment of the lungs (Immunohistochemistry, BaseScope in situ hybridisation) were performed post-mortem. APPV status did not impact virological or immunological differences in PRRSV-infected groups. However, significantly higher rectal temperatures were observed in the APPV+ve/PRRSV+ve group over four days, indicating APPV increased the febrile response. Significant differences in the lung consolidation of the apical and intermediate lobes were also present, suggesting that APPV co-infection may augment lung pathology.
EEA Mercedes
Fil: Hill, Holly. Moredun Research Institute; Reino Unido
Fil: Reddick, David. Moredun Scientific; Reino Unido
Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido
Fil: Ramage, Clifford. Moredun Scientific; Reino Unido
Fil: Frew, David. Moredun Research Institute; Reino Unido
Fil: Rocchi, Mara S. Moredun Research Institute; Reino Unido
Fil: Opriessnig, Tanja. Moredun Research Institute; Reino Unido
Fil: Opriessnig, Tanja. Iowa State University. College of Veterinary Medicine. Department of Veterinary Diagnostic and Production Animal Medicine; Estados Unidos
Fil: McNeilly, Tom Nathan. Moredun Research Institute; Reino Unido
Fuente
Virus Research 348 : 199443. (October 2024)
Materia
Cerdo
Virus Peste Porcina
Reproducción Animal
Enfermedades Respiratorias
Swine
Pestivirus
Swine Fever Virus
Animal Reproduction
Respiratory Diseases
Atypical Porcine Pestivirus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18798
Acceder
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oai_identifier_str oai:localhost:20.500.12123/18798
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spelling Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigsHill, HollyReddick, DavidCaspe, Sergio GastonRamage, CliffordFrew, DavidRocchi, Mara S.Opriessnig, TanjaMcNeilly, Tom NathanCerdoVirus Peste PorcinaReproducción AnimalEnfermedades RespiratoriasSwinePestivirusSwine Fever VirusAnimal ReproductionRespiratory DiseasesAtypical Porcine PestivirusAtypical porcine pestivirus (APPV) is a novel member of the Pestivirus genus detected in association with congenital tremor (CT) type A-II outbreaks and from apparently healthy pigs, both as singular infection and as part of multi-pathogen infections. 'Classical' pestiviruses are known to cause immunosuppression of their host, which can increase susceptibility to secondary infections, severely impacting health, welfare, and production. To investigate APPV's effect on the host's immune system and characterise disease outcomes, 12 piglets from a natural APPV CT type A-II outbreak were experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV), a significant porcine pathogen. Rectal temperatures indicating febrile responses, viremia and viral-specific humoral and cellular responses were assessed throughout the study. Pathological assessment of the lungs and APPV-PRRSV co-localisation within the lungs was performed at necropsy. Viral co-localisation and pathological assessment of the lungs (Immunohistochemistry, BaseScope in situ hybridisation) were performed post-mortem. APPV status did not impact virological or immunological differences in PRRSV-infected groups. However, significantly higher rectal temperatures were observed in the APPV+ve/PRRSV+ve group over four days, indicating APPV increased the febrile response. Significant differences in the lung consolidation of the apical and intermediate lobes were also present, suggesting that APPV co-infection may augment lung pathology.EEA MercedesFil: Hill, Holly. Moredun Research Institute; Reino UnidoFil: Reddick, David. Moredun Scientific; Reino UnidoFil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Caspe, Sergio Gaston. Moredun Research Institute; Reino UnidoFil: Ramage, Clifford. Moredun Scientific; Reino UnidoFil: Frew, David. Moredun Research Institute; Reino UnidoFil: Rocchi, Mara S. Moredun Research Institute; Reino UnidoFil: Opriessnig, Tanja. Moredun Research Institute; Reino UnidoFil: Opriessnig, Tanja. Iowa State University. College of Veterinary Medicine. Department of Veterinary Diagnostic and Production Animal Medicine; Estados UnidosFil: McNeilly, Tom Nathan. Moredun Research Institute; Reino UnidoElsevier2024-08-05T11:44:48Z2024-08-05T11:44:48Z2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18798https://www.sciencedirect.com/science/article/pii/S01681702240013690168-1702https://doi.org/10.1016/j.virusres.2024.199443Virus Research 348 : 199443. (October 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:33Zoai:localhost:20.500.12123/18798instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:33.525INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
title Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
spellingShingle Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
Hill, Holly
Cerdo
Virus Peste Porcina
Reproducción Animal
Enfermedades Respiratorias
Swine
Pestivirus
Swine Fever Virus
Animal Reproduction
Respiratory Diseases
Atypical Porcine Pestivirus
title_short Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
title_full Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
title_fullStr Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
title_full_unstemmed Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
title_sort Enhancing the understanding of coinfection outcomes: Impact of natural atypical porcine pestivirus infection on porcine reproductive and respiratory syndrome in pigs
dc.creator.none.fl_str_mv Hill, Holly
Reddick, David
Caspe, Sergio Gaston
Ramage, Clifford
Frew, David
Rocchi, Mara S.
Opriessnig, Tanja
McNeilly, Tom Nathan
author Hill, Holly
author_facet Hill, Holly
Reddick, David
Caspe, Sergio Gaston
Ramage, Clifford
Frew, David
Rocchi, Mara S.
Opriessnig, Tanja
McNeilly, Tom Nathan
author_role author
author2 Reddick, David
Caspe, Sergio Gaston
Ramage, Clifford
Frew, David
Rocchi, Mara S.
Opriessnig, Tanja
McNeilly, Tom Nathan
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cerdo
Virus Peste Porcina
Reproducción Animal
Enfermedades Respiratorias
Swine
Pestivirus
Swine Fever Virus
Animal Reproduction
Respiratory Diseases
Atypical Porcine Pestivirus
topic Cerdo
Virus Peste Porcina
Reproducción Animal
Enfermedades Respiratorias
Swine
Pestivirus
Swine Fever Virus
Animal Reproduction
Respiratory Diseases
Atypical Porcine Pestivirus
dc.description.none.fl_txt_mv Atypical porcine pestivirus (APPV) is a novel member of the Pestivirus genus detected in association with congenital tremor (CT) type A-II outbreaks and from apparently healthy pigs, both as singular infection and as part of multi-pathogen infections. 'Classical' pestiviruses are known to cause immunosuppression of their host, which can increase susceptibility to secondary infections, severely impacting health, welfare, and production. To investigate APPV's effect on the host's immune system and characterise disease outcomes, 12 piglets from a natural APPV CT type A-II outbreak were experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV), a significant porcine pathogen. Rectal temperatures indicating febrile responses, viremia and viral-specific humoral and cellular responses were assessed throughout the study. Pathological assessment of the lungs and APPV-PRRSV co-localisation within the lungs was performed at necropsy. Viral co-localisation and pathological assessment of the lungs (Immunohistochemistry, BaseScope in situ hybridisation) were performed post-mortem. APPV status did not impact virological or immunological differences in PRRSV-infected groups. However, significantly higher rectal temperatures were observed in the APPV+ve/PRRSV+ve group over four days, indicating APPV increased the febrile response. Significant differences in the lung consolidation of the apical and intermediate lobes were also present, suggesting that APPV co-infection may augment lung pathology.
EEA Mercedes
Fil: Hill, Holly. Moredun Research Institute; Reino Unido
Fil: Reddick, David. Moredun Scientific; Reino Unido
Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido
Fil: Ramage, Clifford. Moredun Scientific; Reino Unido
Fil: Frew, David. Moredun Research Institute; Reino Unido
Fil: Rocchi, Mara S. Moredun Research Institute; Reino Unido
Fil: Opriessnig, Tanja. Moredun Research Institute; Reino Unido
Fil: Opriessnig, Tanja. Iowa State University. College of Veterinary Medicine. Department of Veterinary Diagnostic and Production Animal Medicine; Estados Unidos
Fil: McNeilly, Tom Nathan. Moredun Research Institute; Reino Unido
description Atypical porcine pestivirus (APPV) is a novel member of the Pestivirus genus detected in association with congenital tremor (CT) type A-II outbreaks and from apparently healthy pigs, both as singular infection and as part of multi-pathogen infections. 'Classical' pestiviruses are known to cause immunosuppression of their host, which can increase susceptibility to secondary infections, severely impacting health, welfare, and production. To investigate APPV's effect on the host's immune system and characterise disease outcomes, 12 piglets from a natural APPV CT type A-II outbreak were experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV), a significant porcine pathogen. Rectal temperatures indicating febrile responses, viremia and viral-specific humoral and cellular responses were assessed throughout the study. Pathological assessment of the lungs and APPV-PRRSV co-localisation within the lungs was performed at necropsy. Viral co-localisation and pathological assessment of the lungs (Immunohistochemistry, BaseScope in situ hybridisation) were performed post-mortem. APPV status did not impact virological or immunological differences in PRRSV-infected groups. However, significantly higher rectal temperatures were observed in the APPV+ve/PRRSV+ve group over four days, indicating APPV increased the febrile response. Significant differences in the lung consolidation of the apical and intermediate lobes were also present, suggesting that APPV co-infection may augment lung pathology.
publishDate 2024
dc.date.none.fl_str_mv 2024-08-05T11:44:48Z
2024-08-05T11:44:48Z
2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18798
https://www.sciencedirect.com/science/article/pii/S0168170224001369
0168-1702
https://doi.org/10.1016/j.virusres.2024.199443
url http://hdl.handle.net/20.500.12123/18798
https://www.sciencedirect.com/science/article/pii/S0168170224001369
https://doi.org/10.1016/j.virusres.2024.199443
identifier_str_mv 0168-1702
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Virus Research 348 : 199443. (October 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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