Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina

Autores
Serena, Maria Soledad; Cappuccio, Javier Alejandro; Metz, German Ernesto; Aspitia, C.G.; Dibarbora, Marina; Gallo Calderon, Marina Beatriz; Echeverria, Maria Gabriela
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.
EEA Marcos Juárez
Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aspitia, C.G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina.
Fil: Dibarbora, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.
Fil: Gallo Calderon, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Echeverria, Maria Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Heliyon 5 (11) : e02874 (November 2019)
Materia
Cerdo
Enfermedades de los Animales
Parvovirus Porcino
Cerdas
Biotecnología
Inmunología
Swine
Animal Diseases
Porcine Parvovirus
Sows
Biotechnology
Immunology
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6400
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oai_identifier_str oai:localhost:20.500.12123/6400
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in ArgentinaSerena, Maria SoledadCappuccio, Javier AlejandroMetz, German ErnestoAspitia, C.G.Dibarbora, MarinaGallo Calderon, Marina BeatrizEcheverria, Maria GabrielaCerdoEnfermedades de los AnimalesParvovirus PorcinoCerdasBiotecnologíaInmunologíaSwineAnimal DiseasesPorcine ParvovirusSowsBiotechnologyImmunologyArgentinaPorcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.EEA Marcos JuárezFil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aspitia, C.G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina.Fil: Dibarbora, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.Fil: Gallo Calderon, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Echeverria, Maria Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-11-26T14:38:12Z2019-11-26T14:38:12Z2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S2405844019365338http://hdl.handle.net/20.500.12123/64002405-8440https://doi.org/10.1016/j.heliyon.2019.e02874Heliyon 5 (11) : e02874 (November 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:16Zoai:localhost:20.500.12123/6400instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:17.413INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
spellingShingle Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
Serena, Maria Soledad
Cerdo
Enfermedades de los Animales
Parvovirus Porcino
Cerdas
Biotecnología
Inmunología
Swine
Animal Diseases
Porcine Parvovirus
Sows
Biotechnology
Immunology
Argentina
title_short Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title_full Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title_fullStr Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title_full_unstemmed Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title_sort Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
dc.creator.none.fl_str_mv Serena, Maria Soledad
Cappuccio, Javier Alejandro
Metz, German Ernesto
Aspitia, C.G.
Dibarbora, Marina
Gallo Calderon, Marina Beatriz
Echeverria, Maria Gabriela
author Serena, Maria Soledad
author_facet Serena, Maria Soledad
Cappuccio, Javier Alejandro
Metz, German Ernesto
Aspitia, C.G.
Dibarbora, Marina
Gallo Calderon, Marina Beatriz
Echeverria, Maria Gabriela
author_role author
author2 Cappuccio, Javier Alejandro
Metz, German Ernesto
Aspitia, C.G.
Dibarbora, Marina
Gallo Calderon, Marina Beatriz
Echeverria, Maria Gabriela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cerdo
Enfermedades de los Animales
Parvovirus Porcino
Cerdas
Biotecnología
Inmunología
Swine
Animal Diseases
Porcine Parvovirus
Sows
Biotechnology
Immunology
Argentina
topic Cerdo
Enfermedades de los Animales
Parvovirus Porcino
Cerdas
Biotecnología
Inmunología
Swine
Animal Diseases
Porcine Parvovirus
Sows
Biotechnology
Immunology
Argentina
dc.description.none.fl_txt_mv Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.
EEA Marcos Juárez
Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aspitia, C.G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina.
Fil: Dibarbora, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.
Fil: Gallo Calderon, Marina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Echeverria, Maria Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-26T14:38:12Z
2019-11-26T14:38:12Z
2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S2405844019365338
http://hdl.handle.net/20.500.12123/6400
2405-8440
https://doi.org/10.1016/j.heliyon.2019.e02874
url https://www.sciencedirect.com/science/article/pii/S2405844019365338
http://hdl.handle.net/20.500.12123/6400
https://doi.org/10.1016/j.heliyon.2019.e02874
identifier_str_mv 2405-8440
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Heliyon 5 (11) : e02874 (November 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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