Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina

Autores
Serena, María Soledad; Cappuccio, Javier Alejandro; Metz, Germán Ernesto; Aspitia, Carolina Gabriela; Dibárbora, M.; Gallo Calderón, M.; Echeverría, María Gabriela
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Biotechnology
Immunology
Microbiology
Molecular biology
Veterinary medicine
Porcine parvovirus
Reproductive failures
Molecular characterization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107349
Acceder
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network_name_str SEDICI (UNLP)
spelling Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in ArgentinaSerena, María SoledadCappuccio, Javier AlejandroMetz, Germán ErnestoAspitia, Carolina GabrielaDibárbora, M.Gallo Calderón, M.Echeverría, María GabrielaCiencias VeterinariasBiotechnologyImmunologyMicrobiologyMolecular biologyVeterinary medicinePorcine parvovirusReproductive failuresMolecular characterizationPorcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.Facultad de Ciencias Veterinarias2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/107349enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6881615&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/2405-8440info:eu-repo/semantics/altIdentifier/pmid/31799463info:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2019.e02874info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:56:06Zoai:sedici.unlp.edu.ar:10915/107349Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:56:06.858SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
spellingShingle Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
Serena, María Soledad
Ciencias Veterinarias
Biotechnology
Immunology
Microbiology
Molecular biology
Veterinary medicine
Porcine parvovirus
Reproductive failures
Molecular characterization
title_short Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title_full Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title_fullStr Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title_full_unstemmed Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
title_sort Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina
dc.creator.none.fl_str_mv Serena, María Soledad
Cappuccio, Javier Alejandro
Metz, Germán Ernesto
Aspitia, Carolina Gabriela
Dibárbora, M.
Gallo Calderón, M.
Echeverría, María Gabriela
author Serena, María Soledad
author_facet Serena, María Soledad
Cappuccio, Javier Alejandro
Metz, Germán Ernesto
Aspitia, Carolina Gabriela
Dibárbora, M.
Gallo Calderón, M.
Echeverría, María Gabriela
author_role author
author2 Cappuccio, Javier Alejandro
Metz, Germán Ernesto
Aspitia, Carolina Gabriela
Dibárbora, M.
Gallo Calderón, M.
Echeverría, María Gabriela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Biotechnology
Immunology
Microbiology
Molecular biology
Veterinary medicine
Porcine parvovirus
Reproductive failures
Molecular characterization
topic Ciencias Veterinarias
Biotechnology
Immunology
Microbiology
Molecular biology
Veterinary medicine
Porcine parvovirus
Reproductive failures
Molecular characterization
dc.description.none.fl_txt_mv Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.
Facultad de Ciencias Veterinarias
description Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107349
url http://sedici.unlp.edu.ar/handle/10915/107349
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6881615&blobtype=pdf
info:eu-repo/semantics/altIdentifier/issn/2405-8440
info:eu-repo/semantics/altIdentifier/pmid/31799463
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2019.e02874
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
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collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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