Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows

Autores
Quintero Rodríguez, Luis; Domínguez, Germán; Alvarado Pinedo, María; Travería, Gabriel; Moré, Gastón; Campero, Lucia Maria; De la Sota, Luzbel; Madoz, Laura; Giuliodori, Mauricio
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives of this study were: 1- to evaluate the association of Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Neospora caninum (N. caninum) with the risk for Late Embryonic Loss (LEL) in grazing dairy cows, 2- to evaluate blood progesterone concentration at the time of LEL occurrence, and 3- to describe a novel ultrasound-guided technique for conceptus sampling. We run a prospective cohort study involving 92 cows (46 LEL and 46 NLEL). An LEL cow was that having an embryo with no heartbeat, detached membranes, or floating structures, including embryo remnants detected at pregnancy check by ultrasonography (US) 28–42 days post-AI, whereas an NLEL cow was that with embryo heartbeats detectable by US at pregnancy check 28–42 d post-IA. We took two blood samples from every cow at pregnancy check by US (the day of LEL detection) and 28 d later to perform serological diagnosis of BVDV, BoHV-1, and N. caninum; and to measure blood progesterone concentration at pregnancy check (28–42 d post-AI). We also sampled the conceptus from all the LEL cows. We performed PCR to detect BVDV, BoHV-1, and N. caninum in sampled conceptuses from LEL cows. Finally, we evaluated the associations of risk factors (serological titers, seroconversion, and progesterone) with LEL odds with logistic models. The risk for LEL was associated with serological titers to BVDV (P = 0.03) and tended to be associated with seroconversion to BVDV, given that 19.6% (9/46) in LEL and 6.5% (3/46) in NLEL cows seroconverted to BVDV (P = 0.09). In addition, BVDV was detected in conceptuses from LEL cows that seroconverted to BVDV but not in LEL cows that did not seroconvert. Conversely, the risk for LEL was not associated with the titers or seroconversion to BoHV-1 and N. caninum. BoHV-1 and N. caninum were not identified in any of the conceptuses. Finally, blood progesterone concentration was similar in LEL and NLEL cows, and it was not associated with the risk for LEL (P = 0.54). In conclusion, BVDV infection is a risk factor for LEL in dairy cows.
EEA Balcarce
Fil: Quintero Rodríguez, Luis. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Quintero Rodríguez, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Domínguez, Germán. Actividad Privada; Argentina.
Fil: Alvarado Pinedo, María. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Moré, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Campero, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: De la Sota. Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: De la Sota. Luzbel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Madoz, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Madoz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Giuliodori, Mauricio. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fuente
Theriogenology 194 : 126-132 (December 2022)
Materia
Pestivirus de la Diarrea Bovina
Herpes Virus Bovino
Neospora caninum
Progesterona
Vacas Lecheras
Bovine Diarrhoea Pestivirus
Bovine Herpesvirus
Progesterone
Dairy Cows
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15193
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15193
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cowsQuintero Rodríguez, LuisDomínguez, GermánAlvarado Pinedo, MaríaTravería, GabrielMoré, GastónCampero, Lucia MariaDe la Sota, LuzbelMadoz, LauraGiuliodori, MauricioPestivirus de la Diarrea BovinaHerpes Virus BovinoNeospora caninumProgesteronaVacas LecherasBovine Diarrhoea PestivirusBovine HerpesvirusProgesteroneDairy CowsThe objectives of this study were: 1- to evaluate the association of Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Neospora caninum (N. caninum) with the risk for Late Embryonic Loss (LEL) in grazing dairy cows, 2- to evaluate blood progesterone concentration at the time of LEL occurrence, and 3- to describe a novel ultrasound-guided technique for conceptus sampling. We run a prospective cohort study involving 92 cows (46 LEL and 46 NLEL). An LEL cow was that having an embryo with no heartbeat, detached membranes, or floating structures, including embryo remnants detected at pregnancy check by ultrasonography (US) 28–42 days post-AI, whereas an NLEL cow was that with embryo heartbeats detectable by US at pregnancy check 28–42 d post-IA. We took two blood samples from every cow at pregnancy check by US (the day of LEL detection) and 28 d later to perform serological diagnosis of BVDV, BoHV-1, and N. caninum; and to measure blood progesterone concentration at pregnancy check (28–42 d post-AI). We also sampled the conceptus from all the LEL cows. We performed PCR to detect BVDV, BoHV-1, and N. caninum in sampled conceptuses from LEL cows. Finally, we evaluated the associations of risk factors (serological titers, seroconversion, and progesterone) with LEL odds with logistic models. The risk for LEL was associated with serological titers to BVDV (P = 0.03) and tended to be associated with seroconversion to BVDV, given that 19.6% (9/46) in LEL and 6.5% (3/46) in NLEL cows seroconverted to BVDV (P = 0.09). In addition, BVDV was detected in conceptuses from LEL cows that seroconverted to BVDV but not in LEL cows that did not seroconvert. Conversely, the risk for LEL was not associated with the titers or seroconversion to BoHV-1 and N. caninum. BoHV-1 and N. caninum were not identified in any of the conceptuses. Finally, blood progesterone concentration was similar in LEL and NLEL cows, and it was not associated with the risk for LEL (P = 0.54). In conclusion, BVDV infection is a risk factor for LEL in dairy cows.EEA BalcarceFil: Quintero Rodríguez, Luis. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Fil: Quintero Rodríguez, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Domínguez, Germán. Actividad Privada; Argentina.Fil: Alvarado Pinedo, María. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Fil: Moré, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Campero, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Fil: De la Sota. Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Fil: De la Sota. Luzbel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Madoz, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Fil: Madoz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Giuliodori, Mauricio. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Elsevier2023-09-13T11:05:26Z2023-09-13T11:05:26Z2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15193https://www.sciencedirect.com/science/article/pii/S0093691X220040090093-691X (print)1879-3231 (online)https://doi.org/10.1016/j.theriogenology.2022.10.002Theriogenology 194 : 126-132 (December 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:05Zoai:localhost:20.500.12123/15193instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:05.791INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
title Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
spellingShingle Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
Quintero Rodríguez, Luis
Pestivirus de la Diarrea Bovina
Herpes Virus Bovino
Neospora caninum
Progesterona
Vacas Lecheras
Bovine Diarrhoea Pestivirus
Bovine Herpesvirus
Progesterone
Dairy Cows
title_short Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
title_full Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
title_fullStr Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
title_full_unstemmed Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
title_sort Association of bovine viral diarrhea virus, bovine herpesvirus 1, and Neospora caninum with late embryonic losses in highly supplemented grazing dairy cows
dc.creator.none.fl_str_mv Quintero Rodríguez, Luis
Domínguez, Germán
Alvarado Pinedo, María
Travería, Gabriel
Moré, Gastón
Campero, Lucia Maria
De la Sota, Luzbel
Madoz, Laura
Giuliodori, Mauricio
author Quintero Rodríguez, Luis
author_facet Quintero Rodríguez, Luis
Domínguez, Germán
Alvarado Pinedo, María
Travería, Gabriel
Moré, Gastón
Campero, Lucia Maria
De la Sota, Luzbel
Madoz, Laura
Giuliodori, Mauricio
author_role author
author2 Domínguez, Germán
Alvarado Pinedo, María
Travería, Gabriel
Moré, Gastón
Campero, Lucia Maria
De la Sota, Luzbel
Madoz, Laura
Giuliodori, Mauricio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pestivirus de la Diarrea Bovina
Herpes Virus Bovino
Neospora caninum
Progesterona
Vacas Lecheras
Bovine Diarrhoea Pestivirus
Bovine Herpesvirus
Progesterone
Dairy Cows
topic Pestivirus de la Diarrea Bovina
Herpes Virus Bovino
Neospora caninum
Progesterona
Vacas Lecheras
Bovine Diarrhoea Pestivirus
Bovine Herpesvirus
Progesterone
Dairy Cows
dc.description.none.fl_txt_mv The objectives of this study were: 1- to evaluate the association of Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Neospora caninum (N. caninum) with the risk for Late Embryonic Loss (LEL) in grazing dairy cows, 2- to evaluate blood progesterone concentration at the time of LEL occurrence, and 3- to describe a novel ultrasound-guided technique for conceptus sampling. We run a prospective cohort study involving 92 cows (46 LEL and 46 NLEL). An LEL cow was that having an embryo with no heartbeat, detached membranes, or floating structures, including embryo remnants detected at pregnancy check by ultrasonography (US) 28–42 days post-AI, whereas an NLEL cow was that with embryo heartbeats detectable by US at pregnancy check 28–42 d post-IA. We took two blood samples from every cow at pregnancy check by US (the day of LEL detection) and 28 d later to perform serological diagnosis of BVDV, BoHV-1, and N. caninum; and to measure blood progesterone concentration at pregnancy check (28–42 d post-AI). We also sampled the conceptus from all the LEL cows. We performed PCR to detect BVDV, BoHV-1, and N. caninum in sampled conceptuses from LEL cows. Finally, we evaluated the associations of risk factors (serological titers, seroconversion, and progesterone) with LEL odds with logistic models. The risk for LEL was associated with serological titers to BVDV (P = 0.03) and tended to be associated with seroconversion to BVDV, given that 19.6% (9/46) in LEL and 6.5% (3/46) in NLEL cows seroconverted to BVDV (P = 0.09). In addition, BVDV was detected in conceptuses from LEL cows that seroconverted to BVDV but not in LEL cows that did not seroconvert. Conversely, the risk for LEL was not associated with the titers or seroconversion to BoHV-1 and N. caninum. BoHV-1 and N. caninum were not identified in any of the conceptuses. Finally, blood progesterone concentration was similar in LEL and NLEL cows, and it was not associated with the risk for LEL (P = 0.54). In conclusion, BVDV infection is a risk factor for LEL in dairy cows.
EEA Balcarce
Fil: Quintero Rodríguez, Luis. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Quintero Rodríguez, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Domínguez, Germán. Actividad Privada; Argentina.
Fil: Alvarado Pinedo, María. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Moré, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Campero, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: De la Sota. Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: De la Sota. Luzbel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Madoz, Laura. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Madoz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Giuliodori, Mauricio. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
description The objectives of this study were: 1- to evaluate the association of Bovine Viral Diarrhea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Neospora caninum (N. caninum) with the risk for Late Embryonic Loss (LEL) in grazing dairy cows, 2- to evaluate blood progesterone concentration at the time of LEL occurrence, and 3- to describe a novel ultrasound-guided technique for conceptus sampling. We run a prospective cohort study involving 92 cows (46 LEL and 46 NLEL). An LEL cow was that having an embryo with no heartbeat, detached membranes, or floating structures, including embryo remnants detected at pregnancy check by ultrasonography (US) 28–42 days post-AI, whereas an NLEL cow was that with embryo heartbeats detectable by US at pregnancy check 28–42 d post-IA. We took two blood samples from every cow at pregnancy check by US (the day of LEL detection) and 28 d later to perform serological diagnosis of BVDV, BoHV-1, and N. caninum; and to measure blood progesterone concentration at pregnancy check (28–42 d post-AI). We also sampled the conceptus from all the LEL cows. We performed PCR to detect BVDV, BoHV-1, and N. caninum in sampled conceptuses from LEL cows. Finally, we evaluated the associations of risk factors (serological titers, seroconversion, and progesterone) with LEL odds with logistic models. The risk for LEL was associated with serological titers to BVDV (P = 0.03) and tended to be associated with seroconversion to BVDV, given that 19.6% (9/46) in LEL and 6.5% (3/46) in NLEL cows seroconverted to BVDV (P = 0.09). In addition, BVDV was detected in conceptuses from LEL cows that seroconverted to BVDV but not in LEL cows that did not seroconvert. Conversely, the risk for LEL was not associated with the titers or seroconversion to BoHV-1 and N. caninum. BoHV-1 and N. caninum were not identified in any of the conceptuses. Finally, blood progesterone concentration was similar in LEL and NLEL cows, and it was not associated with the risk for LEL (P = 0.54). In conclusion, BVDV infection is a risk factor for LEL in dairy cows.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2023-09-13T11:05:26Z
2023-09-13T11:05:26Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15193
https://www.sciencedirect.com/science/article/pii/S0093691X22004009
0093-691X (print)
1879-3231 (online)
https://doi.org/10.1016/j.theriogenology.2022.10.002
url http://hdl.handle.net/20.500.12123/15193
https://www.sciencedirect.com/science/article/pii/S0093691X22004009
https://doi.org/10.1016/j.theriogenology.2022.10.002
identifier_str_mv 0093-691X (print)
1879-3231 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Theriogenology 194 : 126-132 (December 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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