Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells

Autores
González Altamiranda, Erika Analía; Kaiser, German Gustavo; Rios, Glenda Laura; Leunda, Maria Rosa; Odeon, Anselmo Carlos
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 ​tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively, and the percentages of oocytes with polar body were analyzed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes, and ZP-intact mature oocytes. These results were confirmed by reverse transcription–polymerase chain reaction. After consecutive washes, the virus remained associated with ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings, it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated with ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection.
EEA Balcarce
Fil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fuente
Theriogenology 86 (8) : 1999-2003 (November 2016)
Materia
Enfermedades de los Animales
Ganado Bovino
Diarrea Viral Bovina
Pestivirus de la Diarrea Bovina
Óvulo
Animal Diseases
Cattle
Bovine Viral Diarrhoea
Bovine Diarrhoea Pestivirus
Ova
Ovocitos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4570
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4570
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cellsGonzález Altamiranda, Erika AnalíaKaiser, German GustavoRios, Glenda LauraLeunda, Maria RosaOdeon, Anselmo CarlosEnfermedades de los AnimalesGanado BovinoDiarrea Viral BovinaPestivirus de la Diarrea BovinaÓvuloAnimal DiseasesCattleBovine Viral DiarrhoeaBovine Diarrhoea PestivirusOvaOvocitosStructural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 ​tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively, and the percentages of oocytes with polar body were analyzed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes, and ZP-intact mature oocytes. These results were confirmed by reverse transcription–polymerase chain reaction. After consecutive washes, the virus remained associated with ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings, it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated with ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection.EEA BalcarceFil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaElsevier2019-03-11T14:25:43Z2019-03-11T14:25:43Z2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0093691X16302783http://hdl.handle.net/20.500.12123/45700093-691X1879-3231https://doi.org/10.1016/j.theriogenology.2016.06.020Theriogenology 86 (8) : 1999-2003 (November 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:35Zoai:localhost:20.500.12123/4570instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:35.88INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
title Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
spellingShingle Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
González Altamiranda, Erika Analía
Enfermedades de los Animales
Ganado Bovino
Diarrea Viral Bovina
Pestivirus de la Diarrea Bovina
Óvulo
Animal Diseases
Cattle
Bovine Viral Diarrhoea
Bovine Diarrhoea Pestivirus
Ova
Ovocitos
title_short Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
title_full Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
title_fullStr Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
title_full_unstemmed Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
title_sort Interaction of bovine viral diarrhea virus with bovine cumulus–oocyte complex during IVM: Detection in permissive cells
dc.creator.none.fl_str_mv González Altamiranda, Erika Analía
Kaiser, German Gustavo
Rios, Glenda Laura
Leunda, Maria Rosa
Odeon, Anselmo Carlos
author González Altamiranda, Erika Analía
author_facet González Altamiranda, Erika Analía
Kaiser, German Gustavo
Rios, Glenda Laura
Leunda, Maria Rosa
Odeon, Anselmo Carlos
author_role author
author2 Kaiser, German Gustavo
Rios, Glenda Laura
Leunda, Maria Rosa
Odeon, Anselmo Carlos
author2_role author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Ganado Bovino
Diarrea Viral Bovina
Pestivirus de la Diarrea Bovina
Óvulo
Animal Diseases
Cattle
Bovine Viral Diarrhoea
Bovine Diarrhoea Pestivirus
Ova
Ovocitos
topic Enfermedades de los Animales
Ganado Bovino
Diarrea Viral Bovina
Pestivirus de la Diarrea Bovina
Óvulo
Animal Diseases
Cattle
Bovine Viral Diarrhoea
Bovine Diarrhoea Pestivirus
Ova
Ovocitos
dc.description.none.fl_txt_mv Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 ​tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively, and the percentages of oocytes with polar body were analyzed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes, and ZP-intact mature oocytes. These results were confirmed by reverse transcription–polymerase chain reaction. After consecutive washes, the virus remained associated with ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings, it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated with ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection.
EEA Balcarce
Fil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
description Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to noncytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus–oocyte complexes were exposed to 107 ​tissue culture-infective doses (TCID50)/mL of an ncp-BVDV strain during IVM (in vitro maturation). After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively, and the percentages of oocytes with polar body were analyzed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes, and ZP-intact mature oocytes. These results were confirmed by reverse transcription–polymerase chain reaction. After consecutive washes, the virus remained associated with ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings, it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated with ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
2019-03-11T14:25:43Z
2019-03-11T14:25:43Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0093691X16302783
http://hdl.handle.net/20.500.12123/4570
0093-691X
1879-3231
https://doi.org/10.1016/j.theriogenology.2016.06.020
url https://www.sciencedirect.com/science/article/pii/S0093691X16302783
http://hdl.handle.net/20.500.12123/4570
https://doi.org/10.1016/j.theriogenology.2016.06.020
identifier_str_mv 0093-691X
1879-3231
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Theriogenology 86 (8) : 1999-2003 (November 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.558318

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