In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model
- Autores
- Quintana, Maria Eugenia; Barone, Lucas Jose; Trotta, Myrian Vanesa; Turco, Cecilia Soledad; Mansilla, Florencia Celeste; Capozzo, Alejandra; Cardoso, Nancy
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bovine-viral-diarrhea virus (BVDV) can cause significant economic losses in livestock. The disease is controlled with vaccination and bovines are susceptible until vaccine immunity develops and may remain vulnerable if a persistently infected animal is left on the farm; therefore, an antiviral agent that reduces virus infectivity can be a useful tool in control programs. Although many compounds with promising in-vitro efficacy have been identified, the lack of laboratory-animal models limited their potential for further clinical development. Recently, we described the activity of type I and III interferons, IFN-α and IFN-λ respectively, against several BVDV strains in-vitro. In this study, we analyzed the in-vivo efficacy of both IFNs using a BALB/c-mouse model. Mice infected with two type-2 BVDV field strains developed a viremia with different kinetics, depending on the infecting strain's virulence, that persisted for 56 days post-infection (dpi). Mice infected with the low-virulence strain elicited high systemic TNF-α levels at 2 dpi. IFNs were first applied subcutaneously 1 day before or after infection. The two IFNs reduced viremia with different kinetics, depending on whether either one was applied before or after infection. In a second experiment, we increased the number of applications of both IFNs. All the treatments reduced viremia compared to untreated mice. The application of IFN-λ pre- and post-infection reduced viremia over time. This study is the first proof of the concept of the antiviral potency of IFN-λ against BVDV in-vivo, thus encouraging further trails for a potential use of this cytokine in cattle.
Instituto de Virología
Fil: Quintana, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Barone, Lucas Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Trotta, Myrian Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Turco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Quintana, María Eugenia. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fil: Barone, Lucas Jose. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Capozzo, Alejandra Victoria.Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina - Fuente
- Frontiers in veterinary science 7 : 45. (Febrero 2020)
- Materia
-
Bovine Diarrhoea Pestivirus
Interferons
Antiviral Agents
Pestivirus de la Diarrea Bovina
Interferonas
Viricidas
Mouse Model
Modelo de Ratón - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/8606
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In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse modelQuintana, Maria EugeniaBarone, Lucas JoseTrotta, Myrian VanesaTurco, Cecilia SoledadMansilla, Florencia CelesteCapozzo, AlejandraCardoso, NancyBovine Diarrhoea PestivirusInterferonsAntiviral AgentsPestivirus de la Diarrea BovinaInterferonasViricidasMouse ModelModelo de RatónBovine-viral-diarrhea virus (BVDV) can cause significant economic losses in livestock. The disease is controlled with vaccination and bovines are susceptible until vaccine immunity develops and may remain vulnerable if a persistently infected animal is left on the farm; therefore, an antiviral agent that reduces virus infectivity can be a useful tool in control programs. Although many compounds with promising in-vitro efficacy have been identified, the lack of laboratory-animal models limited their potential for further clinical development. Recently, we described the activity of type I and III interferons, IFN-α and IFN-λ respectively, against several BVDV strains in-vitro. In this study, we analyzed the in-vivo efficacy of both IFNs using a BALB/c-mouse model. Mice infected with two type-2 BVDV field strains developed a viremia with different kinetics, depending on the infecting strain's virulence, that persisted for 56 days post-infection (dpi). Mice infected with the low-virulence strain elicited high systemic TNF-α levels at 2 dpi. IFNs were first applied subcutaneously 1 day before or after infection. The two IFNs reduced viremia with different kinetics, depending on whether either one was applied before or after infection. In a second experiment, we increased the number of applications of both IFNs. All the treatments reduced viremia compared to untreated mice. The application of IFN-λ pre- and post-infection reduced viremia over time. This study is the first proof of the concept of the antiviral potency of IFN-λ against BVDV in-vivo, thus encouraging further trails for a potential use of this cytokine in cattle.Instituto de VirologíaFil: Quintana, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Barone, Lucas Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Trotta, Myrian Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Turco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Quintana, María Eugenia. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); ArgentinaFil: Barone, Lucas Jose. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Capozzo, Alejandra Victoria.Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); ArgentinaFil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); ArgentinaFrontiers Media2021-01-15T15:11:54Z2021-01-15T15:11:54Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8606https://www.frontiersin.org/articles/10.3389/fvets.2020.00045/full2297-1769https://doi.org/10.3389/fvets.2020.00045Frontiers in veterinary science 7 : 45. (Febrero 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:07Zoai:localhost:20.500.12123/8606instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:07.653INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model |
title |
In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model |
spellingShingle |
In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model Quintana, Maria Eugenia Bovine Diarrhoea Pestivirus Interferons Antiviral Agents Pestivirus de la Diarrea Bovina Interferonas Viricidas Mouse Model Modelo de Ratón |
title_short |
In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model |
title_full |
In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model |
title_fullStr |
In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model |
title_full_unstemmed |
In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model |
title_sort |
In-vivo activity of IFN-λ and IFN-α against bovine-viral-diarrhea virus in a mouse model |
dc.creator.none.fl_str_mv |
Quintana, Maria Eugenia Barone, Lucas Jose Trotta, Myrian Vanesa Turco, Cecilia Soledad Mansilla, Florencia Celeste Capozzo, Alejandra Cardoso, Nancy |
author |
Quintana, Maria Eugenia |
author_facet |
Quintana, Maria Eugenia Barone, Lucas Jose Trotta, Myrian Vanesa Turco, Cecilia Soledad Mansilla, Florencia Celeste Capozzo, Alejandra Cardoso, Nancy |
author_role |
author |
author2 |
Barone, Lucas Jose Trotta, Myrian Vanesa Turco, Cecilia Soledad Mansilla, Florencia Celeste Capozzo, Alejandra Cardoso, Nancy |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Bovine Diarrhoea Pestivirus Interferons Antiviral Agents Pestivirus de la Diarrea Bovina Interferonas Viricidas Mouse Model Modelo de Ratón |
topic |
Bovine Diarrhoea Pestivirus Interferons Antiviral Agents Pestivirus de la Diarrea Bovina Interferonas Viricidas Mouse Model Modelo de Ratón |
dc.description.none.fl_txt_mv |
Bovine-viral-diarrhea virus (BVDV) can cause significant economic losses in livestock. The disease is controlled with vaccination and bovines are susceptible until vaccine immunity develops and may remain vulnerable if a persistently infected animal is left on the farm; therefore, an antiviral agent that reduces virus infectivity can be a useful tool in control programs. Although many compounds with promising in-vitro efficacy have been identified, the lack of laboratory-animal models limited their potential for further clinical development. Recently, we described the activity of type I and III interferons, IFN-α and IFN-λ respectively, against several BVDV strains in-vitro. In this study, we analyzed the in-vivo efficacy of both IFNs using a BALB/c-mouse model. Mice infected with two type-2 BVDV field strains developed a viremia with different kinetics, depending on the infecting strain's virulence, that persisted for 56 days post-infection (dpi). Mice infected with the low-virulence strain elicited high systemic TNF-α levels at 2 dpi. IFNs were first applied subcutaneously 1 day before or after infection. The two IFNs reduced viremia with different kinetics, depending on whether either one was applied before or after infection. In a second experiment, we increased the number of applications of both IFNs. All the treatments reduced viremia compared to untreated mice. The application of IFN-λ pre- and post-infection reduced viremia over time. This study is the first proof of the concept of the antiviral potency of IFN-λ against BVDV in-vivo, thus encouraging further trails for a potential use of this cytokine in cattle. Instituto de Virología Fil: Quintana, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Barone, Lucas Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Trotta, Myrian Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Turco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Fil: Quintana, María Eugenia. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Barone, Lucas Jose. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Capozzo, Alejandra Victoria.Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina |
description |
Bovine-viral-diarrhea virus (BVDV) can cause significant economic losses in livestock. The disease is controlled with vaccination and bovines are susceptible until vaccine immunity develops and may remain vulnerable if a persistently infected animal is left on the farm; therefore, an antiviral agent that reduces virus infectivity can be a useful tool in control programs. Although many compounds with promising in-vitro efficacy have been identified, the lack of laboratory-animal models limited their potential for further clinical development. Recently, we described the activity of type I and III interferons, IFN-α and IFN-λ respectively, against several BVDV strains in-vitro. In this study, we analyzed the in-vivo efficacy of both IFNs using a BALB/c-mouse model. Mice infected with two type-2 BVDV field strains developed a viremia with different kinetics, depending on the infecting strain's virulence, that persisted for 56 days post-infection (dpi). Mice infected with the low-virulence strain elicited high systemic TNF-α levels at 2 dpi. IFNs were first applied subcutaneously 1 day before or after infection. The two IFNs reduced viremia with different kinetics, depending on whether either one was applied before or after infection. In a second experiment, we increased the number of applications of both IFNs. All the treatments reduced viremia compared to untreated mice. The application of IFN-λ pre- and post-infection reduced viremia over time. This study is the first proof of the concept of the antiviral potency of IFN-λ against BVDV in-vivo, thus encouraging further trails for a potential use of this cytokine in cattle. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02 2021-01-15T15:11:54Z 2021-01-15T15:11:54Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/8606 https://www.frontiersin.org/articles/10.3389/fvets.2020.00045/full 2297-1769 https://doi.org/10.3389/fvets.2020.00045 |
url |
http://hdl.handle.net/20.500.12123/8606 https://www.frontiersin.org/articles/10.3389/fvets.2020.00045/full https://doi.org/10.3389/fvets.2020.00045 |
identifier_str_mv |
2297-1769 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
Frontiers in veterinary science 7 : 45. (Febrero 2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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