Stratification temperature affects the In Vitro germination of immature Prunus embryos

Autores
Daorden, Maria Elena; Marin, Juan Antonio; Arbeloa, Arancha
Año de publicación
2004
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The rapid multiplication of clonal plants is desirable to shorten crossing programs in fruit tree breeding. In vitro stratification and germination allowed us to shorten the time and to improve the germination rates by embryo rescue. In this work, two stratification temperatures (0º and 4ºC) were compared and related to the development of interspecific hybrid embryos. The influence of embryo size was also determined, in order to optimise the process. Data showed that germination took place in 91% of the seeds when the stratification temperature was 4ºC. During stratification, embryo weight and embryo-weight increment were assessed at 30 and 60 days after sowing. Stratification temperature showed a significant effect in both embryo weight and embryo-weight increment 60 days after sowing, while embryo size showed a smaller influence. Embryo culture and stratification at 4 ºC allowed a satisfactory germination of immature seeds produced in crosses from breeding programs.
EEA San Pedro
Fil: Daorden, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Marin, Juan Antonio. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental de Aula Dei. Zaragoza; España
Fil: Arbeloa, Arancha. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental de Aula Dei. Zaragoza; España
Fuente
Acta horticulturae 658 :135-140 (Oct. 2004)
Materia
Prunus
Cultivo de Embriones
Embriones Vegetales
Germinación
Semillas
Frutales
Fitomejoramiento
Embryo Culture
Plant Embryos
Germination
Seeds
Fruit Crops
Plant Breeding
Estratificación in Vitro
Híbridos Interespecíficos
In vitro Stratification
Interspecific Hybrids
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6490
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6490
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Stratification temperature affects the In Vitro germination of immature Prunus embryosDaorden, Maria ElenaMarin, Juan AntonioArbeloa, AranchaPrunusCultivo de EmbrionesEmbriones VegetalesGerminaciónSemillasFrutalesFitomejoramientoEmbryo CulturePlant EmbryosGerminationSeedsFruit CropsPlant BreedingEstratificación in VitroHíbridos InterespecíficosIn vitro StratificationInterspecific HybridsThe rapid multiplication of clonal plants is desirable to shorten crossing programs in fruit tree breeding. In vitro stratification and germination allowed us to shorten the time and to improve the germination rates by embryo rescue. In this work, two stratification temperatures (0º and 4ºC) were compared and related to the development of interspecific hybrid embryos. The influence of embryo size was also determined, in order to optimise the process. Data showed that germination took place in 91% of the seeds when the stratification temperature was 4ºC. During stratification, embryo weight and embryo-weight increment were assessed at 30 and 60 days after sowing. Stratification temperature showed a significant effect in both embryo weight and embryo-weight increment 60 days after sowing, while embryo size showed a smaller influence. Embryo culture and stratification at 4 ºC allowed a satisfactory germination of immature seeds produced in crosses from breeding programs.EEA San PedroFil: Daorden, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Marin, Juan Antonio. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental de Aula Dei. Zaragoza; EspañaFil: Arbeloa, Arancha. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental de Aula Dei. Zaragoza; EspañaISHS2019-12-12T11:11:37Z2019-12-12T11:11:37Z2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.actahort.org/books/658/658_18.htmhttp://hdl.handle.net/20.500.12123/6490978-90-66051-90-40567-75722406-6168https://doi.org/10.17660/ActaHortic.2004.658.18Acta horticulturae 658 :135-140 (Oct. 2004)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repo/semantics/restrictedAccess2025-10-23T11:17:09Zoai:localhost:20.500.12123/6490instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:10.135INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Stratification temperature affects the In Vitro germination of immature Prunus embryos
title Stratification temperature affects the In Vitro germination of immature Prunus embryos
spellingShingle Stratification temperature affects the In Vitro germination of immature Prunus embryos
Daorden, Maria Elena
Prunus
Cultivo de Embriones
Embriones Vegetales
Germinación
Semillas
Frutales
Fitomejoramiento
Embryo Culture
Plant Embryos
Germination
Seeds
Fruit Crops
Plant Breeding
Estratificación in Vitro
Híbridos Interespecíficos
In vitro Stratification
Interspecific Hybrids
title_short Stratification temperature affects the In Vitro germination of immature Prunus embryos
title_full Stratification temperature affects the In Vitro germination of immature Prunus embryos
title_fullStr Stratification temperature affects the In Vitro germination of immature Prunus embryos
title_full_unstemmed Stratification temperature affects the In Vitro germination of immature Prunus embryos
title_sort Stratification temperature affects the In Vitro germination of immature Prunus embryos
dc.creator.none.fl_str_mv Daorden, Maria Elena
Marin, Juan Antonio
Arbeloa, Arancha
author Daorden, Maria Elena
author_facet Daorden, Maria Elena
Marin, Juan Antonio
Arbeloa, Arancha
author_role author
author2 Marin, Juan Antonio
Arbeloa, Arancha
author2_role author
author
dc.subject.none.fl_str_mv Prunus
Cultivo de Embriones
Embriones Vegetales
Germinación
Semillas
Frutales
Fitomejoramiento
Embryo Culture
Plant Embryos
Germination
Seeds
Fruit Crops
Plant Breeding
Estratificación in Vitro
Híbridos Interespecíficos
In vitro Stratification
Interspecific Hybrids
topic Prunus
Cultivo de Embriones
Embriones Vegetales
Germinación
Semillas
Frutales
Fitomejoramiento
Embryo Culture
Plant Embryos
Germination
Seeds
Fruit Crops
Plant Breeding
Estratificación in Vitro
Híbridos Interespecíficos
In vitro Stratification
Interspecific Hybrids
dc.description.none.fl_txt_mv The rapid multiplication of clonal plants is desirable to shorten crossing programs in fruit tree breeding. In vitro stratification and germination allowed us to shorten the time and to improve the germination rates by embryo rescue. In this work, two stratification temperatures (0º and 4ºC) were compared and related to the development of interspecific hybrid embryos. The influence of embryo size was also determined, in order to optimise the process. Data showed that germination took place in 91% of the seeds when the stratification temperature was 4ºC. During stratification, embryo weight and embryo-weight increment were assessed at 30 and 60 days after sowing. Stratification temperature showed a significant effect in both embryo weight and embryo-weight increment 60 days after sowing, while embryo size showed a smaller influence. Embryo culture and stratification at 4 ºC allowed a satisfactory germination of immature seeds produced in crosses from breeding programs.
EEA San Pedro
Fil: Daorden, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Marin, Juan Antonio. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental de Aula Dei. Zaragoza; España
Fil: Arbeloa, Arancha. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental de Aula Dei. Zaragoza; España
description The rapid multiplication of clonal plants is desirable to shorten crossing programs in fruit tree breeding. In vitro stratification and germination allowed us to shorten the time and to improve the germination rates by embryo rescue. In this work, two stratification temperatures (0º and 4ºC) were compared and related to the development of interspecific hybrid embryos. The influence of embryo size was also determined, in order to optimise the process. Data showed that germination took place in 91% of the seeds when the stratification temperature was 4ºC. During stratification, embryo weight and embryo-weight increment were assessed at 30 and 60 days after sowing. Stratification temperature showed a significant effect in both embryo weight and embryo-weight increment 60 days after sowing, while embryo size showed a smaller influence. Embryo culture and stratification at 4 ºC allowed a satisfactory germination of immature seeds produced in crosses from breeding programs.
publishDate 2004
dc.date.none.fl_str_mv 2004
2019-12-12T11:11:37Z
2019-12-12T11:11:37Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.actahort.org/books/658/658_18.htm
http://hdl.handle.net/20.500.12123/6490
978-90-66051-90-4
0567-7572
2406-6168
https://doi.org/10.17660/ActaHortic.2004.658.18
url https://www.actahort.org/books/658/658_18.htm
http://hdl.handle.net/20.500.12123/6490
https://doi.org/10.17660/ActaHortic.2004.658.18
identifier_str_mv 978-90-66051-90-4
0567-7572
2406-6168
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISHS
publisher.none.fl_str_mv ISHS
dc.source.none.fl_str_mv Acta horticulturae 658 :135-140 (Oct. 2004)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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