In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection

Autores
Ynsaurralde Rivolta, Amanda Eugenia; Gambini, Andrés; Alberio, Virgilia; Savy, Virginia; Ratner, Laura Daniela; Guberman, Alejandra; Vazquez Echegaray, Camila; Gismondi, Maria Ines; Curra, Anabella Paola; Bevacqua, Romina Jimena; Salamone, Daniel Felipe
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The efficiency of bovine in vitro embryo production can be significantly improved by splitting embryos at different stages. However, the blastocyst quality of in vitro-produced demi-embryos remains unexplored. The objective of this research was to compare embryo developmental rates and quality of bovine demi-embryos produced by two different strategies: (a) embryo bisection (BSEC) and (b) 2-cell blastomere separation (BSEP). To determine demi-embryos quality, we evaluated total blastocyst cell number and proportion of SOX2+ cells. Additionally, the expression of SOX2, NANOG, OCT4, CDX2, IFNT, BAX and BCL genes and let-7a and miRNA-30c Micro RNAs was analysed. BSEP resulted in improved blastocyst development, higher ICM cells and a significantly higher expression of IFNΤ than demi-embryos produced by BSEC. Let-7a, which is associated with low pregnancy establishment was detected in BSEC, while miRNA-30c expression was observed in all treatments. In conclusion, BSEP of 2-cell embryos is more efficient to improve in vitro bovine embryo development and to produce good quality demi-embryos based on ICM cell number and the expression pattern of the genes explored compared to BSEC.
EEA Mercedes
Fil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina.
Fil: Ynsaurralde Rivolta, Amada Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina.
Fil: Gambini, Andrés. The University of Queensland. School of Agriculture and Food Sustainability; Australia
Fil: Gambini, Andrés. The University of Queensland. School of Veterinary Science; Australia
Fil: Alberio, Virgilia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Alberio, Virgilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción Animal; Argentina
Fil: Alberio, Virgilia. Universidad de Buenos Aires. Instituto de Investigaciones en Producción Animal; Argentina
Fil: Savy, Virginia. Reproductive and Developmental Biology Laboratory; Estados Unidos
Fil: Ratner, L. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Guberman, Alejandra S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. IQUIBICEN; Argentina
Fil: Guberman, Alejandra S. Consejo Nacional de Investigaciones Científicas y Técnicas. IQUIBICEN; Argentina
Fil: Vazquez Echegaray, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. IQUIBICEN; Argentina
Fil: Vazquez Echegaray, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. IQUIBICEN; Argentina
Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gismondi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Curra, Anabella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bevacqua, Romina Jimena. Mount Sinai. Department of Medicine; Estados Unidos
Fil: Salamone, Daniel. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Salamone, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción Animal; Argentina
Fil: Salamone, Daniel. Universidad de Buenos Aires. Instituto de Investigaciones en Producción Animal; Argentina
Fuente
Reproduction in Domestic Animals 59 (6) : e14627. (June 2024)
Materia
Embriones Animales
Ganado Bovino
Experimentación in Vitro
Genética
Animal Embryos
Cattle
In Vitro Experimentation
Genetics
Blastocysts
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18064
Acceder
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oai_identifier_str oai:localhost:20.500.12123/18064
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisectionYnsaurralde Rivolta, Amanda EugeniaGambini, AndrésAlberio, VirgiliaSavy, VirginiaRatner, Laura DanielaGuberman, AlejandraVazquez Echegaray, CamilaGismondi, Maria InesCurra, Anabella PaolaBevacqua, Romina JimenaSalamone, Daniel FelipeEmbriones AnimalesGanado BovinoExperimentación in VitroGenéticaAnimal EmbryosCattleIn Vitro ExperimentationGeneticsBlastocystsThe efficiency of bovine in vitro embryo production can be significantly improved by splitting embryos at different stages. However, the blastocyst quality of in vitro-produced demi-embryos remains unexplored. The objective of this research was to compare embryo developmental rates and quality of bovine demi-embryos produced by two different strategies: (a) embryo bisection (BSEC) and (b) 2-cell blastomere separation (BSEP). To determine demi-embryos quality, we evaluated total blastocyst cell number and proportion of SOX2+ cells. Additionally, the expression of SOX2, NANOG, OCT4, CDX2, IFNT, BAX and BCL genes and let-7a and miRNA-30c Micro RNAs was analysed. BSEP resulted in improved blastocyst development, higher ICM cells and a significantly higher expression of IFNΤ than demi-embryos produced by BSEC. Let-7a, which is associated with low pregnancy establishment was detected in BSEC, while miRNA-30c expression was observed in all treatments. In conclusion, BSEP of 2-cell embryos is more efficient to improve in vitro bovine embryo development and to produce good quality demi-embryos based on ICM cell number and the expression pattern of the genes explored compared to BSEC.EEA MercedesFil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina.Fil: Ynsaurralde Rivolta, Amada Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina.Fil: Gambini, Andrés. The University of Queensland. School of Agriculture and Food Sustainability; AustraliaFil: Gambini, Andrés. The University of Queensland. School of Veterinary Science; AustraliaFil: Alberio, Virgilia. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Alberio, Virgilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción Animal; ArgentinaFil: Alberio, Virgilia. Universidad de Buenos Aires. Instituto de Investigaciones en Producción Animal; ArgentinaFil: Savy, Virginia. Reproductive and Developmental Biology Laboratory; Estados UnidosFil: Ratner, L. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Guberman, Alejandra S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. IQUIBICEN; ArgentinaFil: Guberman, Alejandra S. Consejo Nacional de Investigaciones Científicas y Técnicas. IQUIBICEN; ArgentinaFil: Vazquez Echegaray, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. IQUIBICEN; ArgentinaFil: Vazquez Echegaray, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. IQUIBICEN; ArgentinaFil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gismondi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Curra, Anabella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bevacqua, Romina Jimena. Mount Sinai. Department of Medicine; Estados UnidosFil: Salamone, Daniel. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Salamone, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción Animal; ArgentinaFil: Salamone, Daniel. Universidad de Buenos Aires. Instituto de Investigaciones en Producción Animal; ArgentinaWiley2024-06-07T11:48:43Z2024-06-07T11:48:43Z2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18064https://onlinelibrary.wiley.com/doi/10.1111/rda.146270936-67681439-0531https://doi.org/10.1111/rda.14627Reproduction in Domestic Animals 59 (6) : e14627. (June 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:25Zoai:localhost:20.500.12123/18064instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:25.635INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection
title In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection
spellingShingle In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection
Ynsaurralde Rivolta, Amanda Eugenia
Embriones Animales
Ganado Bovino
Experimentación in Vitro
Genética
Animal Embryos
Cattle
In Vitro Experimentation
Genetics
Blastocysts
title_short In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection
title_full In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection
title_fullStr In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection
title_full_unstemmed In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection
title_sort In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection
dc.creator.none.fl_str_mv Ynsaurralde Rivolta, Amanda Eugenia
Gambini, Andrés
Alberio, Virgilia
Savy, Virginia
Ratner, Laura Daniela
Guberman, Alejandra
Vazquez Echegaray, Camila
Gismondi, Maria Ines
Curra, Anabella Paola
Bevacqua, Romina Jimena
Salamone, Daniel Felipe
author Ynsaurralde Rivolta, Amanda Eugenia
author_facet Ynsaurralde Rivolta, Amanda Eugenia
Gambini, Andrés
Alberio, Virgilia
Savy, Virginia
Ratner, Laura Daniela
Guberman, Alejandra
Vazquez Echegaray, Camila
Gismondi, Maria Ines
Curra, Anabella Paola
Bevacqua, Romina Jimena
Salamone, Daniel Felipe
author_role author
author2 Gambini, Andrés
Alberio, Virgilia
Savy, Virginia
Ratner, Laura Daniela
Guberman, Alejandra
Vazquez Echegaray, Camila
Gismondi, Maria Ines
Curra, Anabella Paola
Bevacqua, Romina Jimena
Salamone, Daniel Felipe
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Embriones Animales
Ganado Bovino
Experimentación in Vitro
Genética
Animal Embryos
Cattle
In Vitro Experimentation
Genetics
Blastocysts
topic Embriones Animales
Ganado Bovino
Experimentación in Vitro
Genética
Animal Embryos
Cattle
In Vitro Experimentation
Genetics
Blastocysts
dc.description.none.fl_txt_mv The efficiency of bovine in vitro embryo production can be significantly improved by splitting embryos at different stages. However, the blastocyst quality of in vitro-produced demi-embryos remains unexplored. The objective of this research was to compare embryo developmental rates and quality of bovine demi-embryos produced by two different strategies: (a) embryo bisection (BSEC) and (b) 2-cell blastomere separation (BSEP). To determine demi-embryos quality, we evaluated total blastocyst cell number and proportion of SOX2+ cells. Additionally, the expression of SOX2, NANOG, OCT4, CDX2, IFNT, BAX and BCL genes and let-7a and miRNA-30c Micro RNAs was analysed. BSEP resulted in improved blastocyst development, higher ICM cells and a significantly higher expression of IFNΤ than demi-embryos produced by BSEC. Let-7a, which is associated with low pregnancy establishment was detected in BSEC, while miRNA-30c expression was observed in all treatments. In conclusion, BSEP of 2-cell embryos is more efficient to improve in vitro bovine embryo development and to produce good quality demi-embryos based on ICM cell number and the expression pattern of the genes explored compared to BSEC.
EEA Mercedes
Fil: Ynsaurralde Rivolta, Amada Eugenia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina.
Fil: Ynsaurralde Rivolta, Amada Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina.
Fil: Gambini, Andrés. The University of Queensland. School of Agriculture and Food Sustainability; Australia
Fil: Gambini, Andrés. The University of Queensland. School of Veterinary Science; Australia
Fil: Alberio, Virgilia. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Alberio, Virgilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción Animal; Argentina
Fil: Alberio, Virgilia. Universidad de Buenos Aires. Instituto de Investigaciones en Producción Animal; Argentina
Fil: Savy, Virginia. Reproductive and Developmental Biology Laboratory; Estados Unidos
Fil: Ratner, L. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Guberman, Alejandra S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. IQUIBICEN; Argentina
Fil: Guberman, Alejandra S. Consejo Nacional de Investigaciones Científicas y Técnicas. IQUIBICEN; Argentina
Fil: Vazquez Echegaray, Camila. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. IQUIBICEN; Argentina
Fil: Vazquez Echegaray, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. IQUIBICEN; Argentina
Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gismondi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Curra, Anabella Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bevacqua, Romina Jimena. Mount Sinai. Department of Medicine; Estados Unidos
Fil: Salamone, Daniel. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Salamone, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Producción Animal; Argentina
Fil: Salamone, Daniel. Universidad de Buenos Aires. Instituto de Investigaciones en Producción Animal; Argentina
description The efficiency of bovine in vitro embryo production can be significantly improved by splitting embryos at different stages. However, the blastocyst quality of in vitro-produced demi-embryos remains unexplored. The objective of this research was to compare embryo developmental rates and quality of bovine demi-embryos produced by two different strategies: (a) embryo bisection (BSEC) and (b) 2-cell blastomere separation (BSEP). To determine demi-embryos quality, we evaluated total blastocyst cell number and proportion of SOX2+ cells. Additionally, the expression of SOX2, NANOG, OCT4, CDX2, IFNT, BAX and BCL genes and let-7a and miRNA-30c Micro RNAs was analysed. BSEP resulted in improved blastocyst development, higher ICM cells and a significantly higher expression of IFNΤ than demi-embryos produced by BSEC. Let-7a, which is associated with low pregnancy establishment was detected in BSEC, while miRNA-30c expression was observed in all treatments. In conclusion, BSEP of 2-cell embryos is more efficient to improve in vitro bovine embryo development and to produce good quality demi-embryos based on ICM cell number and the expression pattern of the genes explored compared to BSEC.
publishDate 2024
dc.date.none.fl_str_mv 2024-06-07T11:48:43Z
2024-06-07T11:48:43Z
2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18064
https://onlinelibrary.wiley.com/doi/10.1111/rda.14627
0936-6768
1439-0531
https://doi.org/10.1111/rda.14627
url http://hdl.handle.net/20.500.12123/18064
https://onlinelibrary.wiley.com/doi/10.1111/rda.14627
https://doi.org/10.1111/rda.14627
identifier_str_mv 0936-6768
1439-0531
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Reproduction in Domestic Animals 59 (6) : e14627. (June 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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