Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies

Autores
Capetti, Caio; Arnoldi Pellegrini, Vanessa; Espirito Santo, Melissa; Cortez, Anelyse Abreu; Falvo, Maurício; da Silva Curvelo, Antonio Aprigio; Campos, Eleonora; Filgueiras, Jefferson Gonçalves; Guimaraes, Francisco E.G.; Azevedo, Eduardo R. de; Polikarpov, Igor
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via “green” and sustainable XOS production.
Instituto de Biotecnología
Fil: Capetti, Caio. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Arnoldi Pellegrini, Vanessa. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Espirito Santo, Melissa. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Cortez, Anelyse Abreu. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Falvo, Maurício. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: da Silva Curvelo, Antonio Aprigio. Universidade de São Paulo. Instituto de Química de São Carlos; Brasil
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Filgueiras, Jefferson Gonçalves. Universidade Federal Fluminense. Instituto de Química; Brasil
Fil: Filgueiras, Jefferson Gonçalves. Universidade Federal do Rio de Janeiro. Instituto de Física; Brasil
Fil: Guimaraes, Francisco E.G. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Azevedo, Eduardo R. de. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Polikarpov, Igor. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fuente
Carbohydrate Polymers 299 : 120174 (January 2023)
Materia
Maize cobs
Hydrothermal activity
Alkali treatment
Enzyme activity
Coronta de maíz
Actividad hidrotermal
Tratamiento alcalino
Actividad enzimática
Xylooligosaccharides
Xylanase
Xylosidase
Pretreatment
Pretratamiento
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23242
Acceder
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oai_identifier_str oai:localhost:20.500.12123/23242
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repository_id_str l
network_name_str INTA Digital (INTA)
spelling Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategiesCapetti, CaioArnoldi Pellegrini, VanessaEspirito Santo, MelissaCortez, Anelyse AbreuFalvo, Maurícioda Silva Curvelo, Antonio AprigioCampos, EleonoraFilgueiras, Jefferson GonçalvesGuimaraes, Francisco E.G.Azevedo, Eduardo R. dePolikarpov, IgorMaize cobsHydrothermal activityAlkali treatmentEnzyme activityCoronta de maízActividad hidrotermalTratamiento alcalinoActividad enzimáticaXylooligosaccharidesXylanaseXylosidasePretreatmentPretratamientoCorn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via “green” and sustainable XOS production.Instituto de BiotecnologíaFil: Capetti, Caio. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Arnoldi Pellegrini, Vanessa. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Espirito Santo, Melissa. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Cortez, Anelyse Abreu. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Falvo, Maurício. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: da Silva Curvelo, Antonio Aprigio. Universidade de São Paulo. Instituto de Química de São Carlos; BrasilFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Filgueiras, Jefferson Gonçalves. Universidade Federal Fluminense. Instituto de Química; BrasilFil: Filgueiras, Jefferson Gonçalves. Universidade Federal do Rio de Janeiro. Instituto de Física; BrasilFil: Guimaraes, Francisco E.G. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Azevedo, Eduardo R. de. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Polikarpov, Igor. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilElsevier2025-07-30T17:16:49Z2025-07-30T17:16:49Z2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23242https://www.sciencedirect.com/science/article/pii/S01448617220107971879-1344https://doi.org/10.1016/j.carbpol.2022.120174Carbohydrate Polymers 299 : 120174 (January 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:26Zoai:localhost:20.500.12123/23242instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:27.254INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies
title Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies
spellingShingle Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies
Capetti, Caio
Maize cobs
Hydrothermal activity
Alkali treatment
Enzyme activity
Coronta de maíz
Actividad hidrotermal
Tratamiento alcalino
Actividad enzimática
Xylooligosaccharides
Xylanase
Xylosidase
Pretreatment
Pretratamiento
title_short Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies
title_full Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies
title_fullStr Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies
title_full_unstemmed Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies
title_sort Enzymatic production of xylooligosaccharides from corn cobs : Assessment of two different pretreatment strategies
dc.creator.none.fl_str_mv Capetti, Caio
Arnoldi Pellegrini, Vanessa
Espirito Santo, Melissa
Cortez, Anelyse Abreu
Falvo, Maurício
da Silva Curvelo, Antonio Aprigio
Campos, Eleonora
Filgueiras, Jefferson Gonçalves
Guimaraes, Francisco E.G.
Azevedo, Eduardo R. de
Polikarpov, Igor
author Capetti, Caio
author_facet Capetti, Caio
Arnoldi Pellegrini, Vanessa
Espirito Santo, Melissa
Cortez, Anelyse Abreu
Falvo, Maurício
da Silva Curvelo, Antonio Aprigio
Campos, Eleonora
Filgueiras, Jefferson Gonçalves
Guimaraes, Francisco E.G.
Azevedo, Eduardo R. de
Polikarpov, Igor
author_role author
author2 Arnoldi Pellegrini, Vanessa
Espirito Santo, Melissa
Cortez, Anelyse Abreu
Falvo, Maurício
da Silva Curvelo, Antonio Aprigio
Campos, Eleonora
Filgueiras, Jefferson Gonçalves
Guimaraes, Francisco E.G.
Azevedo, Eduardo R. de
Polikarpov, Igor
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Maize cobs
Hydrothermal activity
Alkali treatment
Enzyme activity
Coronta de maíz
Actividad hidrotermal
Tratamiento alcalino
Actividad enzimática
Xylooligosaccharides
Xylanase
Xylosidase
Pretreatment
Pretratamiento
topic Maize cobs
Hydrothermal activity
Alkali treatment
Enzyme activity
Coronta de maíz
Actividad hidrotermal
Tratamiento alcalino
Actividad enzimática
Xylooligosaccharides
Xylanase
Xylosidase
Pretreatment
Pretratamiento
dc.description.none.fl_txt_mv Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via “green” and sustainable XOS production.
Instituto de Biotecnología
Fil: Capetti, Caio. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Arnoldi Pellegrini, Vanessa. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Espirito Santo, Melissa. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Cortez, Anelyse Abreu. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Falvo, Maurício. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: da Silva Curvelo, Antonio Aprigio. Universidade de São Paulo. Instituto de Química de São Carlos; Brasil
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Filgueiras, Jefferson Gonçalves. Universidade Federal Fluminense. Instituto de Química; Brasil
Fil: Filgueiras, Jefferson Gonçalves. Universidade Federal do Rio de Janeiro. Instituto de Física; Brasil
Fil: Guimaraes, Francisco E.G. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Azevedo, Eduardo R. de. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Polikarpov, Igor. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
description Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via “green” and sustainable XOS production.
publishDate 2023
dc.date.none.fl_str_mv 2023-01
2025-07-30T17:16:49Z
2025-07-30T17:16:49Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23242
https://www.sciencedirect.com/science/article/pii/S0144861722010797
1879-1344
https://doi.org/10.1016/j.carbpol.2022.120174
url http://hdl.handle.net/20.500.12123/23242
https://www.sciencedirect.com/science/article/pii/S0144861722010797
https://doi.org/10.1016/j.carbpol.2022.120174
identifier_str_mv 1879-1344
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Carbohydrate Polymers 299 : 120174 (January 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619207263649792
score 12.559606

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