Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies

Autores
de Mello Capetti, Caio Cesar; Oliveira Arnoldi Pellegrini, Vanessa; Espirito Santo, Melissa Cristina; Abreu Cortez, Anelyse; Falvo, Maurício; Aprigio da Silva Curvelo, Antonio; Campos, Eleonora; Gonçalves Filgueiras, Jefferson; Gontijo Guimaraes, Francisco Eduardo; Ribeiro de Azevedo, Eduardo; Polikarpov, Igor
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via “green” and sustainable XOS production.
Fil: de Mello Capetti, Caio Cesar. Universidade de Sao Paulo; Brasil
Fil: Oliveira Arnoldi Pellegrini, Vanessa. Universidade de Sao Paulo; Brasil
Fil: Espirito Santo, Melissa Cristina. Universidade de Sao Paulo; Brasil
Fil: Abreu Cortez, Anelyse. Universidade de Sao Paulo; Brasil
Fil: Falvo, Maurício. Universidade de Sao Paulo; Brasil
Fil: Aprigio da Silva Curvelo, Antonio. Universidade de Sao Paulo; Brasil
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gonçalves Filgueiras, Jefferson. Universidade Federal Fluminense; Brasil. Universidade Federal do Rio de Janeiro; Brasil
Fil: Gontijo Guimaraes, Francisco Eduardo. Universidade de Sao Paulo; Brasil
Fil: Ribeiro de Azevedo, Eduardo. Universidade de Sao Paulo; Brasil
Fil: Polikarpov, Igor. Universidade de Sao Paulo; Brasil
Materia
ALKALI PRETREATMENT
CORN COBS
HYDROTHERMAL PRETREATMENT
XYLANASE
XYLOOLIGOSACCHARIDES
XYLOSIDASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229061
Acceder
id CONICETDig_6a3a0fcc9f5c8253304f490fce78773a
oai_identifier_str oai:ri.conicet.gov.ar:11336/229061
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategiesde Mello Capetti, Caio CesarOliveira Arnoldi Pellegrini, VanessaEspirito Santo, Melissa CristinaAbreu Cortez, AnelyseFalvo, MaurícioAprigio da Silva Curvelo, AntonioCampos, EleonoraGonçalves Filgueiras, JeffersonGontijo Guimaraes, Francisco EduardoRibeiro de Azevedo, EduardoPolikarpov, IgorALKALI PRETREATMENTCORN COBSHYDROTHERMAL PRETREATMENTXYLANASEXYLOOLIGOSACCHARIDESXYLOSIDASEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via “green” and sustainable XOS production.Fil: de Mello Capetti, Caio Cesar. Universidade de Sao Paulo; BrasilFil: Oliveira Arnoldi Pellegrini, Vanessa. Universidade de Sao Paulo; BrasilFil: Espirito Santo, Melissa Cristina. Universidade de Sao Paulo; BrasilFil: Abreu Cortez, Anelyse. Universidade de Sao Paulo; BrasilFil: Falvo, Maurício. Universidade de Sao Paulo; BrasilFil: Aprigio da Silva Curvelo, Antonio. Universidade de Sao Paulo; BrasilFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gonçalves Filgueiras, Jefferson. Universidade Federal Fluminense; Brasil. Universidade Federal do Rio de Janeiro; BrasilFil: Gontijo Guimaraes, Francisco Eduardo. Universidade de Sao Paulo; BrasilFil: Ribeiro de Azevedo, Eduardo. Universidade de Sao Paulo; BrasilFil: Polikarpov, Igor. Universidade de Sao Paulo; BrasilElsevier2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229061de Mello Capetti, Caio Cesar; Oliveira Arnoldi Pellegrini, Vanessa; Espirito Santo, Melissa Cristina; Abreu Cortez, Anelyse; Falvo, Maurício; et al.; Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies; Elsevier; Carbohydrate Polymers; 299; 1-2023; 1-430144-8617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861722010797info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2022.120174info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:53Zoai:ri.conicet.gov.ar:11336/229061instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:53.494CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies
title Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies
spellingShingle Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies
de Mello Capetti, Caio Cesar
ALKALI PRETREATMENT
CORN COBS
HYDROTHERMAL PRETREATMENT
XYLANASE
XYLOOLIGOSACCHARIDES
XYLOSIDASE
title_short Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies
title_full Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies
title_fullStr Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies
title_full_unstemmed Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies
title_sort Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies
dc.creator.none.fl_str_mv de Mello Capetti, Caio Cesar
Oliveira Arnoldi Pellegrini, Vanessa
Espirito Santo, Melissa Cristina
Abreu Cortez, Anelyse
Falvo, Maurício
Aprigio da Silva Curvelo, Antonio
Campos, Eleonora
Gonçalves Filgueiras, Jefferson
Gontijo Guimaraes, Francisco Eduardo
Ribeiro de Azevedo, Eduardo
Polikarpov, Igor
author de Mello Capetti, Caio Cesar
author_facet de Mello Capetti, Caio Cesar
Oliveira Arnoldi Pellegrini, Vanessa
Espirito Santo, Melissa Cristina
Abreu Cortez, Anelyse
Falvo, Maurício
Aprigio da Silva Curvelo, Antonio
Campos, Eleonora
Gonçalves Filgueiras, Jefferson
Gontijo Guimaraes, Francisco Eduardo
Ribeiro de Azevedo, Eduardo
Polikarpov, Igor
author_role author
author2 Oliveira Arnoldi Pellegrini, Vanessa
Espirito Santo, Melissa Cristina
Abreu Cortez, Anelyse
Falvo, Maurício
Aprigio da Silva Curvelo, Antonio
Campos, Eleonora
Gonçalves Filgueiras, Jefferson
Gontijo Guimaraes, Francisco Eduardo
Ribeiro de Azevedo, Eduardo
Polikarpov, Igor
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALKALI PRETREATMENT
CORN COBS
HYDROTHERMAL PRETREATMENT
XYLANASE
XYLOOLIGOSACCHARIDES
XYLOSIDASE
topic ALKALI PRETREATMENT
CORN COBS
HYDROTHERMAL PRETREATMENT
XYLANASE
XYLOOLIGOSACCHARIDES
XYLOSIDASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via “green” and sustainable XOS production.
Fil: de Mello Capetti, Caio Cesar. Universidade de Sao Paulo; Brasil
Fil: Oliveira Arnoldi Pellegrini, Vanessa. Universidade de Sao Paulo; Brasil
Fil: Espirito Santo, Melissa Cristina. Universidade de Sao Paulo; Brasil
Fil: Abreu Cortez, Anelyse. Universidade de Sao Paulo; Brasil
Fil: Falvo, Maurício. Universidade de Sao Paulo; Brasil
Fil: Aprigio da Silva Curvelo, Antonio. Universidade de Sao Paulo; Brasil
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gonçalves Filgueiras, Jefferson. Universidade Federal Fluminense; Brasil. Universidade Federal do Rio de Janeiro; Brasil
Fil: Gontijo Guimaraes, Francisco Eduardo. Universidade de Sao Paulo; Brasil
Fil: Ribeiro de Azevedo, Eduardo. Universidade de Sao Paulo; Brasil
Fil: Polikarpov, Igor. Universidade de Sao Paulo; Brasil
description Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via “green” and sustainable XOS production.
publishDate 2023
dc.date.none.fl_str_mv 2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229061
de Mello Capetti, Caio Cesar; Oliveira Arnoldi Pellegrini, Vanessa; Espirito Santo, Melissa Cristina; Abreu Cortez, Anelyse; Falvo, Maurício; et al.; Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies; Elsevier; Carbohydrate Polymers; 299; 1-2023; 1-43
0144-8617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229061
identifier_str_mv de Mello Capetti, Caio Cesar; Oliveira Arnoldi Pellegrini, Vanessa; Espirito Santo, Melissa Cristina; Abreu Cortez, Anelyse; Falvo, Maurício; et al.; Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies; Elsevier; Carbohydrate Polymers; 299; 1-2023; 1-43
0144-8617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0144861722010797
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2022.120174
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613412411146240
score 13.070432

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