Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes

Autores
Capetti, Caio; Arnoldi Pellegrini, Vanessa; Vacilotto, Milena Moreira; da Silva Curvelo, Antonio Aprigio; Falvo, Maurício; Guimaraes, Francisco E.G.; Ontañon, Ornella Mailen; Campos, Eleonora; Polikarpov, Igor
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are beneficial for human and animal health. In this study, two alternative pretreatment strategies (alkali and hydrothermal) and three different enzymes were applied for enzymatic XOS production from sugarcane bagasse. The enzymatic hydrolysis was performed with nine different combinations of recombinant endo-xylanases from GH11 and GH10 families and GH11 xylobiohydrolase. Hydrothermal pretreatment followed by optimized enzymatic hydrolysis yielded up to 96 ± 1 mg of XOS per gram of initial biomass, whereas enzymatic hydrolysis of alkali-pretreated sugarcane bagasse rendered around 47.6 ± 0.2 mg/g. For both alkali and hydrothermal routes, the maximum yields of short-length XOS were obtained using the GH10 xylanase alone. Furthermore, differences in XOS profiles obtained by controlled mixtures of the enzymes have been evaluated. For both routes, the best yields of short-length XOS were obtained using the GH10 xylanase alone, which is consistent with the notion that sugarcane xylan substitutions partially hinder GH11 xylanase activity. The results presented here show that a green and cost-effective hydrothermal pretreatment path for xylooligosaccharides production, rendered considerably better XOS yields.
Instituto de Biotecnología
Fil: Capetti, Caio. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Arnoldi Pellegrini, Vanessa. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Vacilotto, Milena Moreira. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: da Silva Curvelo, Antonio Aprigio. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Falvo, Maurício. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Guimaraes, Francisco E.G. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Polikarpov, Igor. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fuente
Food and Bioprocess Technology : 1-13 (Published: 18 October 2023)
Materia
Hydrothermal Activity
Sugar Cane
Bagasse
Enzymes
Oligosaccharides
Xylans
Actividad Hidrotermal
Caña de Azúcar
Bagazo
Enzimas
Oligosacáridos
Xilanos
Alkali Pretreatment
Xylooligosaccharides
Pretratamiento Alcalino
Xilooligosacáridos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16388
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16388
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymesCapetti, CaioArnoldi Pellegrini, VanessaVacilotto, Milena Moreirada Silva Curvelo, Antonio AprigioFalvo, MaurícioGuimaraes, Francisco E.G.Ontañon, Ornella MailenCampos, EleonoraPolikarpov, IgorHydrothermal ActivitySugar CaneBagasseEnzymesOligosaccharidesXylansActividad HidrotermalCaña de AzúcarBagazoEnzimasOligosacáridosXilanosAlkali PretreatmentXylooligosaccharidesPretratamiento AlcalinoXilooligosacáridosXylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are beneficial for human and animal health. In this study, two alternative pretreatment strategies (alkali and hydrothermal) and three different enzymes were applied for enzymatic XOS production from sugarcane bagasse. The enzymatic hydrolysis was performed with nine different combinations of recombinant endo-xylanases from GH11 and GH10 families and GH11 xylobiohydrolase. Hydrothermal pretreatment followed by optimized enzymatic hydrolysis yielded up to 96 ± 1 mg of XOS per gram of initial biomass, whereas enzymatic hydrolysis of alkali-pretreated sugarcane bagasse rendered around 47.6 ± 0.2 mg/g. For both alkali and hydrothermal routes, the maximum yields of short-length XOS were obtained using the GH10 xylanase alone. Furthermore, differences in XOS profiles obtained by controlled mixtures of the enzymes have been evaluated. For both routes, the best yields of short-length XOS were obtained using the GH10 xylanase alone, which is consistent with the notion that sugarcane xylan substitutions partially hinder GH11 xylanase activity. The results presented here show that a green and cost-effective hydrothermal pretreatment path for xylooligosaccharides production, rendered considerably better XOS yields.Instituto de BiotecnologíaFil: Capetti, Caio. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Arnoldi Pellegrini, Vanessa. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Vacilotto, Milena Moreira. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: da Silva Curvelo, Antonio Aprigio. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Falvo, Maurício. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Guimaraes, Francisco E.G. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilFil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Polikarpov, Igor. Universidade de São Paulo. Instituto de Física de São Carlos; BrasilSpringer2023-12-28T16:03:02Z2023-12-28T16:03:02Z2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16388https://link.springer.com/article/10.1007/s11947-023-03226-71935-5149https://doi.org/10.1007/s11947-023-03226-7Food and Bioprocess Technology : 1-13 (Published: 18 October 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:17Zoai:localhost:20.500.12123/16388instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:18.262INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
title Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
spellingShingle Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
Capetti, Caio
Hydrothermal Activity
Sugar Cane
Bagasse
Enzymes
Oligosaccharides
Xylans
Actividad Hidrotermal
Caña de Azúcar
Bagazo
Enzimas
Oligosacáridos
Xilanos
Alkali Pretreatment
Xylooligosaccharides
Pretratamiento Alcalino
Xilooligosacáridos
title_short Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
title_full Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
title_fullStr Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
title_full_unstemmed Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
title_sort Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
dc.creator.none.fl_str_mv Capetti, Caio
Arnoldi Pellegrini, Vanessa
Vacilotto, Milena Moreira
da Silva Curvelo, Antonio Aprigio
Falvo, Maurício
Guimaraes, Francisco E.G.
Ontañon, Ornella Mailen
Campos, Eleonora
Polikarpov, Igor
author Capetti, Caio
author_facet Capetti, Caio
Arnoldi Pellegrini, Vanessa
Vacilotto, Milena Moreira
da Silva Curvelo, Antonio Aprigio
Falvo, Maurício
Guimaraes, Francisco E.G.
Ontañon, Ornella Mailen
Campos, Eleonora
Polikarpov, Igor
author_role author
author2 Arnoldi Pellegrini, Vanessa
Vacilotto, Milena Moreira
da Silva Curvelo, Antonio Aprigio
Falvo, Maurício
Guimaraes, Francisco E.G.
Ontañon, Ornella Mailen
Campos, Eleonora
Polikarpov, Igor
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hydrothermal Activity
Sugar Cane
Bagasse
Enzymes
Oligosaccharides
Xylans
Actividad Hidrotermal
Caña de Azúcar
Bagazo
Enzimas
Oligosacáridos
Xilanos
Alkali Pretreatment
Xylooligosaccharides
Pretratamiento Alcalino
Xilooligosacáridos
topic Hydrothermal Activity
Sugar Cane
Bagasse
Enzymes
Oligosaccharides
Xylans
Actividad Hidrotermal
Caña de Azúcar
Bagazo
Enzimas
Oligosacáridos
Xilanos
Alkali Pretreatment
Xylooligosaccharides
Pretratamiento Alcalino
Xilooligosacáridos
dc.description.none.fl_txt_mv Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are beneficial for human and animal health. In this study, two alternative pretreatment strategies (alkali and hydrothermal) and three different enzymes were applied for enzymatic XOS production from sugarcane bagasse. The enzymatic hydrolysis was performed with nine different combinations of recombinant endo-xylanases from GH11 and GH10 families and GH11 xylobiohydrolase. Hydrothermal pretreatment followed by optimized enzymatic hydrolysis yielded up to 96 ± 1 mg of XOS per gram of initial biomass, whereas enzymatic hydrolysis of alkali-pretreated sugarcane bagasse rendered around 47.6 ± 0.2 mg/g. For both alkali and hydrothermal routes, the maximum yields of short-length XOS were obtained using the GH10 xylanase alone. Furthermore, differences in XOS profiles obtained by controlled mixtures of the enzymes have been evaluated. For both routes, the best yields of short-length XOS were obtained using the GH10 xylanase alone, which is consistent with the notion that sugarcane xylan substitutions partially hinder GH11 xylanase activity. The results presented here show that a green and cost-effective hydrothermal pretreatment path for xylooligosaccharides production, rendered considerably better XOS yields.
Instituto de Biotecnología
Fil: Capetti, Caio. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Arnoldi Pellegrini, Vanessa. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Vacilotto, Milena Moreira. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: da Silva Curvelo, Antonio Aprigio. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Falvo, Maurício. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Guimaraes, Francisco E.G. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
Fil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Polikarpov, Igor. Universidade de São Paulo. Instituto de Física de São Carlos; Brasil
description Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are beneficial for human and animal health. In this study, two alternative pretreatment strategies (alkali and hydrothermal) and three different enzymes were applied for enzymatic XOS production from sugarcane bagasse. The enzymatic hydrolysis was performed with nine different combinations of recombinant endo-xylanases from GH11 and GH10 families and GH11 xylobiohydrolase. Hydrothermal pretreatment followed by optimized enzymatic hydrolysis yielded up to 96 ± 1 mg of XOS per gram of initial biomass, whereas enzymatic hydrolysis of alkali-pretreated sugarcane bagasse rendered around 47.6 ± 0.2 mg/g. For both alkali and hydrothermal routes, the maximum yields of short-length XOS were obtained using the GH10 xylanase alone. Furthermore, differences in XOS profiles obtained by controlled mixtures of the enzymes have been evaluated. For both routes, the best yields of short-length XOS were obtained using the GH10 xylanase alone, which is consistent with the notion that sugarcane xylan substitutions partially hinder GH11 xylanase activity. The results presented here show that a green and cost-effective hydrothermal pretreatment path for xylooligosaccharides production, rendered considerably better XOS yields.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-28T16:03:02Z
2023-12-28T16:03:02Z
2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16388
https://link.springer.com/article/10.1007/s11947-023-03226-7
1935-5149
https://doi.org/10.1007/s11947-023-03226-7
url http://hdl.handle.net/20.500.12123/16388
https://link.springer.com/article/10.1007/s11947-023-03226-7
https://doi.org/10.1007/s11947-023-03226-7
identifier_str_mv 1935-5149
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Food and Bioprocess Technology : 1-13 (Published: 18 October 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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