Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system

Autores
Gómez García, Ricardo; Medina Morales, Miguel A.; Rodrìguez, Raul; Farruggia, Beatriz Monica; Picó, Guillermo Alfredo; Aguilar, Cristóbal N.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Production of xylanase enzyme by fungi strains has gained interest in the recent years due to its high productivity, high catalytic power, as well as its potential applications in different areas such as feed, food, textile, and biofuel industries. The conventional methodologies, to produce enzymes, involve complex apparatus and chemical solvents and are associated with high costs and low-yield recovery. To obtain the high-yield recovery of the enzymes, modern enzyme extraction methods are taken into account. Aqueous two-phase systems (ATPS) are an alternative separative methodology for the purification and recovery of the enzymes and other biomolecules. The advantages of ATPS are easy scale-up and extraction, volume reduction, and rapid separation. The objective of this study was to produce Trichoderma harzianum xylanase by solid-state fermentation (SSF) using corn cobs as a support/substrate and employing ATPS for its partial recovery. In this study, the results showed the ability of a microorganism to grow on the corn cobs and to produce the xylanase enzyme. Xylanolytic activity reached 7.85 U/g of corn cobs. The enzyme was efficiently concentrated by ATPS. In addition, a high purification factor (10-fold) and considerable enzyme recovery (%ER) (84%) percentage were obtained.
Fil: Ricardo GOMEZ GARCIA. Autonomous University Of Coahuila, Saltillo, Coahuila,; México
Fil: Miguel MEDINA-Morales. Autonomous University Of Coahuila, Saltillo, Coahuila,; México
Fil: Raúl RODRIGUEZ. Autonomous University Of Coahuila, Saltillo, Coahuila,; México
Fil: Farruggia, Beatriz Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Cristobal AGUILAR. Autonomous University Of Coahuila, Saltillo, Coahuila,; México
Materia
XYLANASE
TRICHODERMA
SOLID-STATE FERMENTATION
CORN COBS
BIPHASIC SYSTEMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95805
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95805
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase systemGómez García, RicardoMedina Morales, Miguel A.Rodrìguez, RaulFarruggia, Beatriz MonicaPicó, Guillermo AlfredoAguilar, Cristóbal N.XYLANASETRICHODERMASOLID-STATE FERMENTATIONCORN COBSBIPHASIC SYSTEMShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Production of xylanase enzyme by fungi strains has gained interest in the recent years due to its high productivity, high catalytic power, as well as its potential applications in different areas such as feed, food, textile, and biofuel industries. The conventional methodologies, to produce enzymes, involve complex apparatus and chemical solvents and are associated with high costs and low-yield recovery. To obtain the high-yield recovery of the enzymes, modern enzyme extraction methods are taken into account. Aqueous two-phase systems (ATPS) are an alternative separative methodology for the purification and recovery of the enzymes and other biomolecules. The advantages of ATPS are easy scale-up and extraction, volume reduction, and rapid separation. The objective of this study was to produce Trichoderma harzianum xylanase by solid-state fermentation (SSF) using corn cobs as a support/substrate and employing ATPS for its partial recovery. In this study, the results showed the ability of a microorganism to grow on the corn cobs and to produce the xylanase enzyme. Xylanolytic activity reached 7.85 U/g of corn cobs. The enzyme was efficiently concentrated by ATPS. In addition, a high purification factor (10-fold) and considerable enzyme recovery (%ER) (84%) percentage were obtained.Fil: Ricardo GOMEZ GARCIA. Autonomous University Of Coahuila, Saltillo, Coahuila,; MéxicoFil: Miguel MEDINA-Morales. Autonomous University Of Coahuila, Saltillo, Coahuila,; MéxicoFil: Raúl RODRIGUEZ. Autonomous University Of Coahuila, Saltillo, Coahuila,; MéxicoFil: Farruggia, Beatriz Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Cristobal AGUILAR. Autonomous University Of Coahuila, Saltillo, Coahuila,; MéxicoScience Planet2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95805Gómez García, Ricardo; Medina Morales, Miguel A.; Rodrìguez, Raul; Farruggia, Beatriz Monica; Picó, Guillermo Alfredo; et al.; Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system; Science Planet; Canadian Journal of Biotechnology; 2; 2; 9-2018; 108-1152560-8304CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.24870/cjb.2018-000122info:eu-repo/semantics/altIdentifier/url/https://www.canadianjbiotech.com/can-j-biotech/archives/v2/i2/cjb-2018-122.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:54Zoai:ri.conicet.gov.ar:11336/95805instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:54.393CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system
title Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system
spellingShingle Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system
Gómez García, Ricardo
XYLANASE
TRICHODERMA
SOLID-STATE FERMENTATION
CORN COBS
BIPHASIC SYSTEMS
title_short Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system
title_full Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system
title_fullStr Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system
title_full_unstemmed Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system
title_sort Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system
dc.creator.none.fl_str_mv Gómez García, Ricardo
Medina Morales, Miguel A.
Rodrìguez, Raul
Farruggia, Beatriz Monica
Picó, Guillermo Alfredo
Aguilar, Cristóbal N.
author Gómez García, Ricardo
author_facet Gómez García, Ricardo
Medina Morales, Miguel A.
Rodrìguez, Raul
Farruggia, Beatriz Monica
Picó, Guillermo Alfredo
Aguilar, Cristóbal N.
author_role author
author2 Medina Morales, Miguel A.
Rodrìguez, Raul
Farruggia, Beatriz Monica
Picó, Guillermo Alfredo
Aguilar, Cristóbal N.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv XYLANASE
TRICHODERMA
SOLID-STATE FERMENTATION
CORN COBS
BIPHASIC SYSTEMS
topic XYLANASE
TRICHODERMA
SOLID-STATE FERMENTATION
CORN COBS
BIPHASIC SYSTEMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Production of xylanase enzyme by fungi strains has gained interest in the recent years due to its high productivity, high catalytic power, as well as its potential applications in different areas such as feed, food, textile, and biofuel industries. The conventional methodologies, to produce enzymes, involve complex apparatus and chemical solvents and are associated with high costs and low-yield recovery. To obtain the high-yield recovery of the enzymes, modern enzyme extraction methods are taken into account. Aqueous two-phase systems (ATPS) are an alternative separative methodology for the purification and recovery of the enzymes and other biomolecules. The advantages of ATPS are easy scale-up and extraction, volume reduction, and rapid separation. The objective of this study was to produce Trichoderma harzianum xylanase by solid-state fermentation (SSF) using corn cobs as a support/substrate and employing ATPS for its partial recovery. In this study, the results showed the ability of a microorganism to grow on the corn cobs and to produce the xylanase enzyme. Xylanolytic activity reached 7.85 U/g of corn cobs. The enzyme was efficiently concentrated by ATPS. In addition, a high purification factor (10-fold) and considerable enzyme recovery (%ER) (84%) percentage were obtained.
Fil: Ricardo GOMEZ GARCIA. Autonomous University Of Coahuila, Saltillo, Coahuila,; México
Fil: Miguel MEDINA-Morales. Autonomous University Of Coahuila, Saltillo, Coahuila,; México
Fil: Raúl RODRIGUEZ. Autonomous University Of Coahuila, Saltillo, Coahuila,; México
Fil: Farruggia, Beatriz Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Cristobal AGUILAR. Autonomous University Of Coahuila, Saltillo, Coahuila,; México
description Production of xylanase enzyme by fungi strains has gained interest in the recent years due to its high productivity, high catalytic power, as well as its potential applications in different areas such as feed, food, textile, and biofuel industries. The conventional methodologies, to produce enzymes, involve complex apparatus and chemical solvents and are associated with high costs and low-yield recovery. To obtain the high-yield recovery of the enzymes, modern enzyme extraction methods are taken into account. Aqueous two-phase systems (ATPS) are an alternative separative methodology for the purification and recovery of the enzymes and other biomolecules. The advantages of ATPS are easy scale-up and extraction, volume reduction, and rapid separation. The objective of this study was to produce Trichoderma harzianum xylanase by solid-state fermentation (SSF) using corn cobs as a support/substrate and employing ATPS for its partial recovery. In this study, the results showed the ability of a microorganism to grow on the corn cobs and to produce the xylanase enzyme. Xylanolytic activity reached 7.85 U/g of corn cobs. The enzyme was efficiently concentrated by ATPS. In addition, a high purification factor (10-fold) and considerable enzyme recovery (%ER) (84%) percentage were obtained.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95805
Gómez García, Ricardo; Medina Morales, Miguel A.; Rodrìguez, Raul; Farruggia, Beatriz Monica; Picó, Guillermo Alfredo; et al.; Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system; Science Planet; Canadian Journal of Biotechnology; 2; 2; 9-2018; 108-115
2560-8304
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95805
identifier_str_mv Gómez García, Ricardo; Medina Morales, Miguel A.; Rodrìguez, Raul; Farruggia, Beatriz Monica; Picó, Guillermo Alfredo; et al.; Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system; Science Planet; Canadian Journal of Biotechnology; 2; 2; 9-2018; 108-115
2560-8304
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.24870/cjb.2018-000122
info:eu-repo/semantics/altIdentifier/url/https://www.canadianjbiotech.com/can-j-biotech/archives/v2/i2/cjb-2018-122.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Planet
publisher.none.fl_str_mv Science Planet
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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