Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty

Autores
Dal Zotto, Angelica; Balzarini, Mónica Graciela; Raigon, Juan Manuel; Rossini, Mirta Noemi; Ducasse, Daniel Adrian
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; ICRT- PCR amplified a 1.2-kb fragment of the virus genome encoding the CP-3′ nc region. The analysis of the sequence showed the presence of the DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites at the 3′ end. The phylogenetic relationships and multiple alignment with PPV isolates from NCBI database indicated greatest (+98%) homology with the D strain and close identity with MNAT1 (\AF360579) USA peach isolate. The sequence analysed showed two amino acid mutations towards the 5′ N-terminus of CP (the most variable region) with respect to a consensus of PPV D-strain isolates. This is the first molecular characterization of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese plum from Argentina.
Instituto de Patología Vegetal
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Balzarini, Mónica Graciela. Universidad Nacional de Cordoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Raigon, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan. Agencia de Extensión Rural San Martín; Argentina
Fil: Rossini, Mirta Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Journal of phytopathology 162 (1) : 55-60. (January 2014)
Materia
Prunus Salicina
Virus Sharka del Ciruelo
Variedades
Plum Pox Virus
Varieties
Ciruelo Japonés
Argentina
Red Beauty (Variedad)
Relación Filogenética
Phylogenetic Relationships
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4648
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4648
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spelling Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red BeautyDal Zotto, AngelicaBalzarini, Mónica GracielaRaigon, Juan ManuelRossini, Mirta NoemiDucasse, Daniel AdrianPrunus SalicinaVirus Sharka del CirueloVariedadesPlum Pox VirusVarietiesCiruelo JaponésArgentinaRed Beauty (Variedad)Relación FilogenéticaPhylogenetic RelationshipsA Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; ICRT- PCR amplified a 1.2-kb fragment of the virus genome encoding the CP-3′ nc region. The analysis of the sequence showed the presence of the DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites at the 3′ end. The phylogenetic relationships and multiple alignment with PPV isolates from NCBI database indicated greatest (+98%) homology with the D strain and close identity with MNAT1 (\AF360579) USA peach isolate. The sequence analysed showed two amino acid mutations towards the 5′ N-terminus of CP (the most variable region) with respect to a consensus of PPV D-strain isolates. This is the first molecular characterization of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese plum from Argentina.Instituto de Patología VegetalFil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Balzarini, Mónica Graciela. Universidad Nacional de Cordoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Raigon, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan. Agencia de Extensión Rural San Martín; ArgentinaFil: Rossini, Mirta Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; ArgentinaFil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaWiley2019-03-19T12:03:54Z2019-03-19T12:03:54Z2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/46481439-0434 (Online)https://doi.org/10.1111/jph.12160Journal of phytopathology 162 (1) : 55-60. (January 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:52Zoai:localhost:20.500.12123/4648instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:53.234INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
title Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
spellingShingle Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
Dal Zotto, Angelica
Prunus Salicina
Virus Sharka del Ciruelo
Variedades
Plum Pox Virus
Varieties
Ciruelo Japonés
Argentina
Red Beauty (Variedad)
Relación Filogenética
Phylogenetic Relationships
title_short Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
title_full Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
title_fullStr Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
title_full_unstemmed Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
title_sort Plum Pox virus in japanese plum from Argentina: serological detection and molecular characterization of an isolate from cv. Red Beauty
dc.creator.none.fl_str_mv Dal Zotto, Angelica
Balzarini, Mónica Graciela
Raigon, Juan Manuel
Rossini, Mirta Noemi
Ducasse, Daniel Adrian
author Dal Zotto, Angelica
author_facet Dal Zotto, Angelica
Balzarini, Mónica Graciela
Raigon, Juan Manuel
Rossini, Mirta Noemi
Ducasse, Daniel Adrian
author_role author
author2 Balzarini, Mónica Graciela
Raigon, Juan Manuel
Rossini, Mirta Noemi
Ducasse, Daniel Adrian
author2_role author
author
author
author
dc.subject.none.fl_str_mv Prunus Salicina
Virus Sharka del Ciruelo
Variedades
Plum Pox Virus
Varieties
Ciruelo Japonés
Argentina
Red Beauty (Variedad)
Relación Filogenética
Phylogenetic Relationships
topic Prunus Salicina
Virus Sharka del Ciruelo
Variedades
Plum Pox Virus
Varieties
Ciruelo Japonés
Argentina
Red Beauty (Variedad)
Relación Filogenética
Phylogenetic Relationships
dc.description.none.fl_txt_mv A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; ICRT- PCR amplified a 1.2-kb fragment of the virus genome encoding the CP-3′ nc region. The analysis of the sequence showed the presence of the DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites at the 3′ end. The phylogenetic relationships and multiple alignment with PPV isolates from NCBI database indicated greatest (+98%) homology with the D strain and close identity with MNAT1 (\AF360579) USA peach isolate. The sequence analysed showed two amino acid mutations towards the 5′ N-terminus of CP (the most variable region) with respect to a consensus of PPV D-strain isolates. This is the first molecular characterization of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese plum from Argentina.
Instituto de Patología Vegetal
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Balzarini, Mónica Graciela. Universidad Nacional de Cordoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Raigon, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan. Agencia de Extensión Rural San Martín; Argentina
Fil: Rossini, Mirta Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description A Plum pox virus (PPV) isolate detected in a Japanese plum orchard in Pocito (San Juan, Argentina) was transmitted mechanically to Prunus tomentosa and Nicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated the virus presence and serological relationship with D-strain isolates; ICRT- PCR amplified a 1.2-kb fragment of the virus genome encoding the CP-3′ nc region. The analysis of the sequence showed the presence of the DAG motif at the 5′ end of the capsid protein and the Rsa I and Alu I sites at the 3′ end. The phylogenetic relationships and multiple alignment with PPV isolates from NCBI database indicated greatest (+98%) homology with the D strain and close identity with MNAT1 (\AF360579) USA peach isolate. The sequence analysed showed two amino acid mutations towards the 5′ N-terminus of CP (the most variable region) with respect to a consensus of PPV D-strain isolates. This is the first molecular characterization of 3′terminal genome region of PPV isolate to confirm D strain in a Japanese plum from Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
2019-03-19T12:03:54Z
2019-03-19T12:03:54Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4648
1439-0434 (Online)
https://doi.org/10.1111/jph.12160
url http://hdl.handle.net/20.500.12123/4648
https://doi.org/10.1111/jph.12160
identifier_str_mv 1439-0434 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Journal of phytopathology 162 (1) : 55-60. (January 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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