Spiraea sp. new natural host of Plum pox virus (Sharka)

Autores
Piglionico, Damián Gustavo; Ojeda, Eva Maria; Lucero, Vanesa Angelica; Farrando, Roberto Jes; Porcel, Laura Beatriz; Picca, Cecilia Nelida; Marini, Diana Beatriz
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings, were observed on leaves of an ornamental plant of the Rosaceae family “Bridal Crown” (Spiraea sp.) in the garden of a house within the PPV quarantine area in San Rafael, Mendoza province. Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. A 243 bp PCR fragment amplified from the Spiraea samples, and corresponding to the carboxy-terminal region of PPV coat protein gene was isolated, sequenced, and deposited in GenBank (MN587037). It showed 99% identity to other PPV isolates. RT-qPCR, confirmed the virus as being an isolate of the PPV D strain previously found in the quarantine areas. Our results confirm PPV natural infection on a new ornamental host, “Bridal Crown”, which does not belong to the genus Prunus, warning of the potential danger of the species as a host of, and a source of, inoculum for PPV.
EEA Junín
Fil: Piglionico, Damián Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Lucero, Vanesa Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fuente
European Journal of Plant Pathology (Published: 27 January 2021)
Materia
Virus Sharka del Ciruelo
Virus de las Plantas
Spiraea
Huéspedes
Identificación
Plum Pox Virus
Plant Viruses
Hosts
Identification
Sharka
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/8777
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spelling Spiraea sp. new natural host of Plum pox virus (Sharka)Piglionico, Damián GustavoOjeda, Eva MariaLucero, Vanesa AngelicaFarrando, Roberto JesPorcel, Laura BeatrizPicca, Cecilia NelidaMarini, Diana BeatrizVirus Sharka del CirueloVirus de las PlantasSpiraeaHuéspedesIdentificaciónPlum Pox VirusPlant VirusesHostsIdentificationSharkaPlum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings, were observed on leaves of an ornamental plant of the Rosaceae family “Bridal Crown” (Spiraea sp.) in the garden of a house within the PPV quarantine area in San Rafael, Mendoza province. Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. A 243 bp PCR fragment amplified from the Spiraea samples, and corresponding to the carboxy-terminal region of PPV coat protein gene was isolated, sequenced, and deposited in GenBank (MN587037). It showed 99% identity to other PPV isolates. RT-qPCR, confirmed the virus as being an isolate of the PPV D strain previously found in the quarantine areas. Our results confirm PPV natural infection on a new ornamental host, “Bridal Crown”, which does not belong to the genus Prunus, warning of the potential danger of the species as a host of, and a source of, inoculum for PPV.EEA JunínFil: Piglionico, Damián Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Lucero, Vanesa Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaSpringer2021-03-01T18:03:51Z2021-03-01T18:03:51Z2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8777https://link.springer.com/article/10.1007/s10658-021-02206-x0929-18731573-8469https://doi.org/10.1007/s10658-021-02206-xEuropean Journal of Plant Pathology (Published: 27 January 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:01Zoai:localhost:20.500.12123/8777instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:01.56INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Spiraea sp. new natural host of Plum pox virus (Sharka)
title Spiraea sp. new natural host of Plum pox virus (Sharka)
spellingShingle Spiraea sp. new natural host of Plum pox virus (Sharka)
Piglionico, Damián Gustavo
Virus Sharka del Ciruelo
Virus de las Plantas
Spiraea
Huéspedes
Identificación
Plum Pox Virus
Plant Viruses
Hosts
Identification
Sharka
title_short Spiraea sp. new natural host of Plum pox virus (Sharka)
title_full Spiraea sp. new natural host of Plum pox virus (Sharka)
title_fullStr Spiraea sp. new natural host of Plum pox virus (Sharka)
title_full_unstemmed Spiraea sp. new natural host of Plum pox virus (Sharka)
title_sort Spiraea sp. new natural host of Plum pox virus (Sharka)
dc.creator.none.fl_str_mv Piglionico, Damián Gustavo
Ojeda, Eva Maria
Lucero, Vanesa Angelica
Farrando, Roberto Jes
Porcel, Laura Beatriz
Picca, Cecilia Nelida
Marini, Diana Beatriz
author Piglionico, Damián Gustavo
author_facet Piglionico, Damián Gustavo
Ojeda, Eva Maria
Lucero, Vanesa Angelica
Farrando, Roberto Jes
Porcel, Laura Beatriz
Picca, Cecilia Nelida
Marini, Diana Beatriz
author_role author
author2 Ojeda, Eva Maria
Lucero, Vanesa Angelica
Farrando, Roberto Jes
Porcel, Laura Beatriz
Picca, Cecilia Nelida
Marini, Diana Beatriz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Virus Sharka del Ciruelo
Virus de las Plantas
Spiraea
Huéspedes
Identificación
Plum Pox Virus
Plant Viruses
Hosts
Identification
Sharka
topic Virus Sharka del Ciruelo
Virus de las Plantas
Spiraea
Huéspedes
Identificación
Plum Pox Virus
Plant Viruses
Hosts
Identification
Sharka
dc.description.none.fl_txt_mv Plum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings, were observed on leaves of an ornamental plant of the Rosaceae family “Bridal Crown” (Spiraea sp.) in the garden of a house within the PPV quarantine area in San Rafael, Mendoza province. Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. A 243 bp PCR fragment amplified from the Spiraea samples, and corresponding to the carboxy-terminal region of PPV coat protein gene was isolated, sequenced, and deposited in GenBank (MN587037). It showed 99% identity to other PPV isolates. RT-qPCR, confirmed the virus as being an isolate of the PPV D strain previously found in the quarantine areas. Our results confirm PPV natural infection on a new ornamental host, “Bridal Crown”, which does not belong to the genus Prunus, warning of the potential danger of the species as a host of, and a source of, inoculum for PPV.
EEA Junín
Fil: Piglionico, Damián Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Lucero, Vanesa Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
description Plum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings, were observed on leaves of an ornamental plant of the Rosaceae family “Bridal Crown” (Spiraea sp.) in the garden of a house within the PPV quarantine area in San Rafael, Mendoza province. Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. A 243 bp PCR fragment amplified from the Spiraea samples, and corresponding to the carboxy-terminal region of PPV coat protein gene was isolated, sequenced, and deposited in GenBank (MN587037). It showed 99% identity to other PPV isolates. RT-qPCR, confirmed the virus as being an isolate of the PPV D strain previously found in the quarantine areas. Our results confirm PPV natural infection on a new ornamental host, “Bridal Crown”, which does not belong to the genus Prunus, warning of the potential danger of the species as a host of, and a source of, inoculum for PPV.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-01T18:03:51Z
2021-03-01T18:03:51Z
2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8777
https://link.springer.com/article/10.1007/s10658-021-02206-x
0929-1873
1573-8469
https://doi.org/10.1007/s10658-021-02206-x
url http://hdl.handle.net/20.500.12123/8777
https://link.springer.com/article/10.1007/s10658-021-02206-x
https://doi.org/10.1007/s10658-021-02206-x
identifier_str_mv 0929-1873
1573-8469
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European Journal of Plant Pathology (Published: 27 January 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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