Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty

Autores
Dal Zotto, Angélica; Balzarini, Monica Graciela; Raigón, Juan M.; Rossini, Mirta N.; Ducasse, Daniel Adrián
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
APlum pox virus(PPV) isolate detected in a Japanese plum orchard in Poci-to (San Juan, Argentina) was transmitted mechanically toPrunus tomento-saandNicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated thevirus presence and serological relationship with D-strain isolates; IC-RT-PCR amplified a 1.2-kb fragment of the virus genome encoding theCP-3′nc region. The analysis of the sequence showed the presence of theDAG motif at the 5′end of the capsid protein and the Rsa I and Alu I sitesat the 3′end. The phylogenetic relationships and multiple alignment withPPV isolates from NCBI database indicated greatest (+98%) homologywith the D strain and close identity with MNAT1 (\AF360579) USA peachisolate. The sequence analysed showed two amino acid mutations towardsthe 5′N-terminus of CP (the most variable region) with respect to a con-sensus of PPV D-strain isolates. This is the first molecular characterizationof 3′terminal genome region of PPV isolate to confirm D strain in a Japa-nese plum from Argentina
Fil: Dal Zotto, Angélica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina
Fil: Balzarini, Monica Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Raigón, Juan M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Rossini, Mirta N.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ducasse, Daniel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina
Materia
Argentina
Phylogenetic Relationship
Plumpox Virus
Prunus Salicinacv. Red Beauty
Sharka
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33868
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red BeautyDal Zotto, AngélicaBalzarini, Monica GracielaRaigón, Juan M.Rossini, Mirta N.Ducasse, Daniel AdriánArgentinaPhylogenetic RelationshipPlumpox VirusPrunus Salicinacv. Red BeautySharkahttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4APlum pox virus(PPV) isolate detected in a Japanese plum orchard in Poci-to (San Juan, Argentina) was transmitted mechanically toPrunus tomento-saandNicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated thevirus presence and serological relationship with D-strain isolates; IC-RT-PCR amplified a 1.2-kb fragment of the virus genome encoding theCP-3′nc region. The analysis of the sequence showed the presence of theDAG motif at the 5′end of the capsid protein and the Rsa I and Alu I sitesat the 3′end. The phylogenetic relationships and multiple alignment withPPV isolates from NCBI database indicated greatest (+98%) homologywith the D strain and close identity with MNAT1 (\AF360579) USA peachisolate. The sequence analysed showed two amino acid mutations towardsthe 5′N-terminus of CP (the most variable region) with respect to a con-sensus of PPV D-strain isolates. This is the first molecular characterizationof 3′terminal genome region of PPV isolate to confirm D strain in a Japa-nese plum from ArgentinaFil: Dal Zotto, Angélica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; ArgentinaFil: Balzarini, Monica Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Raigón, Juan M.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Rossini, Mirta N.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Ducasse, Daniel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; ArgentinaWiley Blackwell Publishing, Inc2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33868Dal Zotto, Angélica; Balzarini, Monica Graciela; Raigón, Juan M.; Rossini, Mirta N.; Ducasse, Daniel Adrián; Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 162; 1; 1-2014; 55-600931-1785CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jph.12160info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jph.12160/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:55:57Zoai:ri.conicet.gov.ar:11336/33868instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:55:58.053CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
title Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
spellingShingle Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
Dal Zotto, Angélica
Argentina
Phylogenetic Relationship
Plumpox Virus
Prunus Salicinacv. Red Beauty
Sharka
title_short Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
title_full Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
title_fullStr Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
title_full_unstemmed Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
title_sort Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty
dc.creator.none.fl_str_mv Dal Zotto, Angélica
Balzarini, Monica Graciela
Raigón, Juan M.
Rossini, Mirta N.
Ducasse, Daniel Adrián
author Dal Zotto, Angélica
author_facet Dal Zotto, Angélica
Balzarini, Monica Graciela
Raigón, Juan M.
Rossini, Mirta N.
Ducasse, Daniel Adrián
author_role author
author2 Balzarini, Monica Graciela
Raigón, Juan M.
Rossini, Mirta N.
Ducasse, Daniel Adrián
author2_role author
author
author
author
dc.subject.none.fl_str_mv Argentina
Phylogenetic Relationship
Plumpox Virus
Prunus Salicinacv. Red Beauty
Sharka
topic Argentina
Phylogenetic Relationship
Plumpox Virus
Prunus Salicinacv. Red Beauty
Sharka
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv APlum pox virus(PPV) isolate detected in a Japanese plum orchard in Poci-to (San Juan, Argentina) was transmitted mechanically toPrunus tomento-saandNicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated thevirus presence and serological relationship with D-strain isolates; IC-RT-PCR amplified a 1.2-kb fragment of the virus genome encoding theCP-3′nc region. The analysis of the sequence showed the presence of theDAG motif at the 5′end of the capsid protein and the Rsa I and Alu I sitesat the 3′end. The phylogenetic relationships and multiple alignment withPPV isolates from NCBI database indicated greatest (+98%) homologywith the D strain and close identity with MNAT1 (\AF360579) USA peachisolate. The sequence analysed showed two amino acid mutations towardsthe 5′N-terminus of CP (the most variable region) with respect to a con-sensus of PPV D-strain isolates. This is the first molecular characterizationof 3′terminal genome region of PPV isolate to confirm D strain in a Japa-nese plum from Argentina
Fil: Dal Zotto, Angélica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina
Fil: Balzarini, Monica Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Raigón, Juan M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Rossini, Mirta N.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ducasse, Daniel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina
description APlum pox virus(PPV) isolate detected in a Japanese plum orchard in Poci-to (San Juan, Argentina) was transmitted mechanically toPrunus tomento-saandNicotiana benthamiana. DAS-ELISA and DASI-ELISA indicated thevirus presence and serological relationship with D-strain isolates; IC-RT-PCR amplified a 1.2-kb fragment of the virus genome encoding theCP-3′nc region. The analysis of the sequence showed the presence of theDAG motif at the 5′end of the capsid protein and the Rsa I and Alu I sitesat the 3′end. The phylogenetic relationships and multiple alignment withPPV isolates from NCBI database indicated greatest (+98%) homologywith the D strain and close identity with MNAT1 (\AF360579) USA peachisolate. The sequence analysed showed two amino acid mutations towardsthe 5′N-terminus of CP (the most variable region) with respect to a con-sensus of PPV D-strain isolates. This is the first molecular characterizationof 3′terminal genome region of PPV isolate to confirm D strain in a Japa-nese plum from Argentina
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33868
Dal Zotto, Angélica; Balzarini, Monica Graciela; Raigón, Juan M.; Rossini, Mirta N.; Ducasse, Daniel Adrián; Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 162; 1; 1-2014; 55-60
0931-1785
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33868
identifier_str_mv Dal Zotto, Angélica; Balzarini, Monica Graciela; Raigón, Juan M.; Rossini, Mirta N.; Ducasse, Daniel Adrián; Plum Pox Virus in Japanese Plum from Argentina: Serological Detection and Molecular Characterization of an Isolate from cv. Red Beauty; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 162; 1; 1-2014; 55-60
0931-1785
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jph.12160
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jph.12160/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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