Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina

Autores
Marini, Diana Beatriz; Farrando, Roberto Jes; Porcel, Laura Beatriz; Ojeda, Eva Maria; Picca, Cecilia Nelida; Fuentes, C.; Dal Zotto, Angelica; Teich, Ingrid
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
II International Symposium on Plum Pox Virus
Sharka, caused by Plum pox virus (PPV), is the most important viral disease of stone fruits worldwide. PPV was detected for first time in Argentina in 2004 and since then many efforts have been made to eradicate it. To optimize sampling and virus detection by DAS-ELISA, 8 PPV-positive prune trees (P. domestica, cv. 'D´agen') were selected and sampled monthly from September 2008 to August 2010. The plant tissue collected was the one available at the sampling time (leaves, flowers or buds). The samples were taken from each of the four tree scaffolds at three different heights (basal, medium and apical). Variability of absorbance levels in each month was analyzed with Principal Components Analysis and sampling schemes were compared with non parametric variance analysis. This is the first study made in Argentina to tracking PPV relative virus concentration throughout the year. As described previously by many authors in the north hemisphere the highest levels of virus concentration, in both studied years, were found in spring (October and November) using flowers and young leaves. The scaffold basal height showed the highest PPV concentration in these two months. High variation of relative virus concentration levels were found among months, within and between years. Regarding different parts of the plant, relative virus concentration decreased from the base to the apical part of the scaffold, indicating that sampling should be performed at a basal height. This study will contribute to the optimization of DAS-ELISA PPV detection in Argentina.
EEA Junín
Fil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Fuentes, C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Teich, Ingrid. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Acta horticulturae 1063 : 177-183. (January 2015)
Materia
Ciruela
Prunus Domestica
Virus de las Plantas
Virus Sharka del Ciruelo
Plums
Plant Viruses
Plum Pox Virus
ELISA
Argentina
Sharka
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4828

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spelling Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in ArgentinaMarini, Diana BeatrizFarrando, Roberto JesPorcel, Laura BeatrizOjeda, Eva MariaPicca, Cecilia NelidaFuentes, C.Dal Zotto, AngelicaTeich, IngridCiruelaPrunus DomesticaVirus de las PlantasVirus Sharka del CirueloPlumsPlant VirusesPlum Pox VirusELISAArgentinaSharkaII International Symposium on Plum Pox VirusSharka, caused by Plum pox virus (PPV), is the most important viral disease of stone fruits worldwide. PPV was detected for first time in Argentina in 2004 and since then many efforts have been made to eradicate it. To optimize sampling and virus detection by DAS-ELISA, 8 PPV-positive prune trees (P. domestica, cv. 'D´agen') were selected and sampled monthly from September 2008 to August 2010. The plant tissue collected was the one available at the sampling time (leaves, flowers or buds). The samples were taken from each of the four tree scaffolds at three different heights (basal, medium and apical). Variability of absorbance levels in each month was analyzed with Principal Components Analysis and sampling schemes were compared with non parametric variance analysis. This is the first study made in Argentina to tracking PPV relative virus concentration throughout the year. As described previously by many authors in the north hemisphere the highest levels of virus concentration, in both studied years, were found in spring (October and November) using flowers and young leaves. The scaffold basal height showed the highest PPV concentration in these two months. High variation of relative virus concentration levels were found among months, within and between years. Regarding different parts of the plant, relative virus concentration decreased from the base to the apical part of the scaffold, indicating that sampling should be performed at a basal height. This study will contribute to the optimization of DAS-ELISA PPV detection in Argentina.EEA JunínFil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Fuentes, C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Teich, Ingrid. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInternational Society for Horticultural Science2019-04-05T13:47:17Z2019-04-05T13:47:17Z2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4828https://www.ishs.org/ishs-article/1063_250567-75722406-6168 (Online)https://doi.org/10.17660/ActaHortic.2015.1063.25Acta horticulturae 1063 : 177-183. (January 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:55Zoai:localhost:20.500.12123/4828instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:55.601INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
title Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
spellingShingle Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
Marini, Diana Beatriz
Ciruela
Prunus Domestica
Virus de las Plantas
Virus Sharka del Ciruelo
Plums
Plant Viruses
Plum Pox Virus
ELISA
Argentina
Sharka
title_short Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
title_full Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
title_fullStr Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
title_full_unstemmed Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
title_sort Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
dc.creator.none.fl_str_mv Marini, Diana Beatriz
Farrando, Roberto Jes
Porcel, Laura Beatriz
Ojeda, Eva Maria
Picca, Cecilia Nelida
Fuentes, C.
Dal Zotto, Angelica
Teich, Ingrid
author Marini, Diana Beatriz
author_facet Marini, Diana Beatriz
Farrando, Roberto Jes
Porcel, Laura Beatriz
Ojeda, Eva Maria
Picca, Cecilia Nelida
Fuentes, C.
Dal Zotto, Angelica
Teich, Ingrid
author_role author
author2 Farrando, Roberto Jes
Porcel, Laura Beatriz
Ojeda, Eva Maria
Picca, Cecilia Nelida
Fuentes, C.
Dal Zotto, Angelica
Teich, Ingrid
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciruela
Prunus Domestica
Virus de las Plantas
Virus Sharka del Ciruelo
Plums
Plant Viruses
Plum Pox Virus
ELISA
Argentina
Sharka
topic Ciruela
Prunus Domestica
Virus de las Plantas
Virus Sharka del Ciruelo
Plums
Plant Viruses
Plum Pox Virus
ELISA
Argentina
Sharka
dc.description.none.fl_txt_mv II International Symposium on Plum Pox Virus
Sharka, caused by Plum pox virus (PPV), is the most important viral disease of stone fruits worldwide. PPV was detected for first time in Argentina in 2004 and since then many efforts have been made to eradicate it. To optimize sampling and virus detection by DAS-ELISA, 8 PPV-positive prune trees (P. domestica, cv. 'D´agen') were selected and sampled monthly from September 2008 to August 2010. The plant tissue collected was the one available at the sampling time (leaves, flowers or buds). The samples were taken from each of the four tree scaffolds at three different heights (basal, medium and apical). Variability of absorbance levels in each month was analyzed with Principal Components Analysis and sampling schemes were compared with non parametric variance analysis. This is the first study made in Argentina to tracking PPV relative virus concentration throughout the year. As described previously by many authors in the north hemisphere the highest levels of virus concentration, in both studied years, were found in spring (October and November) using flowers and young leaves. The scaffold basal height showed the highest PPV concentration in these two months. High variation of relative virus concentration levels were found among months, within and between years. Regarding different parts of the plant, relative virus concentration decreased from the base to the apical part of the scaffold, indicating that sampling should be performed at a basal height. This study will contribute to the optimization of DAS-ELISA PPV detection in Argentina.
EEA Junín
Fil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Fuentes, C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Teich, Ingrid. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description II International Symposium on Plum Pox Virus
publishDate 2015
dc.date.none.fl_str_mv 2015-01
2019-04-05T13:47:17Z
2019-04-05T13:47:17Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4828
https://www.ishs.org/ishs-article/1063_25
0567-7572
2406-6168 (Online)
https://doi.org/10.17660/ActaHortic.2015.1063.25
url http://hdl.handle.net/20.500.12123/4828
https://www.ishs.org/ishs-article/1063_25
https://doi.org/10.17660/ActaHortic.2015.1063.25
identifier_str_mv 0567-7572
2406-6168 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv International Society for Horticultural Science
publisher.none.fl_str_mv International Society for Horticultural Science
dc.source.none.fl_str_mv Acta horticulturae 1063 : 177-183. (January 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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