Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina
- Autores
- Marini, Diana Beatriz; Farrando, Roberto Jes; Porcel, Laura Beatriz; Ojeda, Eva Maria; Picca, Cecilia Nelida; Fuentes, C.; Dal Zotto, Angelica; Teich, Ingrid
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- II International Symposium on Plum Pox Virus
Sharka, caused by Plum pox virus (PPV), is the most important viral disease of stone fruits worldwide. PPV was detected for first time in Argentina in 2004 and since then many efforts have been made to eradicate it. To optimize sampling and virus detection by DAS-ELISA, 8 PPV-positive prune trees (P. domestica, cv. 'D´agen') were selected and sampled monthly from September 2008 to August 2010. The plant tissue collected was the one available at the sampling time (leaves, flowers or buds). The samples were taken from each of the four tree scaffolds at three different heights (basal, medium and apical). Variability of absorbance levels in each month was analyzed with Principal Components Analysis and sampling schemes were compared with non parametric variance analysis. This is the first study made in Argentina to tracking PPV relative virus concentration throughout the year. As described previously by many authors in the north hemisphere the highest levels of virus concentration, in both studied years, were found in spring (October and November) using flowers and young leaves. The scaffold basal height showed the highest PPV concentration in these two months. High variation of relative virus concentration levels were found among months, within and between years. Regarding different parts of the plant, relative virus concentration decreased from the base to the apical part of the scaffold, indicating that sampling should be performed at a basal height. This study will contribute to the optimization of DAS-ELISA PPV detection in Argentina.
EEA Junín
Fil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina
Fil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Fuentes, C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Teich, Ingrid. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Acta horticulturae 1063 : 177-183. (January 2015)
- Materia
-
Ciruela
Prunus Domestica
Virus de las Plantas
Virus Sharka del Ciruelo
Plums
Plant Viruses
Plum Pox Virus
ELISA
Argentina
Sharka - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4828
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Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in ArgentinaMarini, Diana BeatrizFarrando, Roberto JesPorcel, Laura BeatrizOjeda, Eva MariaPicca, Cecilia NelidaFuentes, C.Dal Zotto, AngelicaTeich, IngridCiruelaPrunus DomesticaVirus de las PlantasVirus Sharka del CirueloPlumsPlant VirusesPlum Pox VirusELISAArgentinaSharkaII International Symposium on Plum Pox VirusSharka, caused by Plum pox virus (PPV), is the most important viral disease of stone fruits worldwide. PPV was detected for first time in Argentina in 2004 and since then many efforts have been made to eradicate it. To optimize sampling and virus detection by DAS-ELISA, 8 PPV-positive prune trees (P. domestica, cv. 'D´agen') were selected and sampled monthly from September 2008 to August 2010. The plant tissue collected was the one available at the sampling time (leaves, flowers or buds). The samples were taken from each of the four tree scaffolds at three different heights (basal, medium and apical). Variability of absorbance levels in each month was analyzed with Principal Components Analysis and sampling schemes were compared with non parametric variance analysis. This is the first study made in Argentina to tracking PPV relative virus concentration throughout the year. As described previously by many authors in the north hemisphere the highest levels of virus concentration, in both studied years, were found in spring (October and November) using flowers and young leaves. The scaffold basal height showed the highest PPV concentration in these two months. High variation of relative virus concentration levels were found among months, within and between years. Regarding different parts of the plant, relative virus concentration decreased from the base to the apical part of the scaffold, indicating that sampling should be performed at a basal height. This study will contribute to the optimization of DAS-ELISA PPV detection in Argentina.EEA JunínFil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; ArgentinaFil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Fuentes, C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; ArgentinaFil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Teich, Ingrid. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInternational Society for Horticultural Science2019-04-05T13:47:17Z2019-04-05T13:47:17Z2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4828https://www.ishs.org/ishs-article/1063_250567-75722406-6168 (Online)https://doi.org/10.17660/ActaHortic.2015.1063.25Acta horticulturae 1063 : 177-183. (January 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:55Zoai:localhost:20.500.12123/4828instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:55.601INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina |
title |
Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina |
spellingShingle |
Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina Marini, Diana Beatriz Ciruela Prunus Domestica Virus de las Plantas Virus Sharka del Ciruelo Plums Plant Viruses Plum Pox Virus ELISA Argentina Sharka |
title_short |
Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina |
title_full |
Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina |
title_fullStr |
Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina |
title_full_unstemmed |
Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina |
title_sort |
Monitoring of Plum Pox Virus concentration at different plant heights throughout the year in prunes (Prunus domestica) in Argentina |
dc.creator.none.fl_str_mv |
Marini, Diana Beatriz Farrando, Roberto Jes Porcel, Laura Beatriz Ojeda, Eva Maria Picca, Cecilia Nelida Fuentes, C. Dal Zotto, Angelica Teich, Ingrid |
author |
Marini, Diana Beatriz |
author_facet |
Marini, Diana Beatriz Farrando, Roberto Jes Porcel, Laura Beatriz Ojeda, Eva Maria Picca, Cecilia Nelida Fuentes, C. Dal Zotto, Angelica Teich, Ingrid |
author_role |
author |
author2 |
Farrando, Roberto Jes Porcel, Laura Beatriz Ojeda, Eva Maria Picca, Cecilia Nelida Fuentes, C. Dal Zotto, Angelica Teich, Ingrid |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Ciruela Prunus Domestica Virus de las Plantas Virus Sharka del Ciruelo Plums Plant Viruses Plum Pox Virus ELISA Argentina Sharka |
topic |
Ciruela Prunus Domestica Virus de las Plantas Virus Sharka del Ciruelo Plums Plant Viruses Plum Pox Virus ELISA Argentina Sharka |
dc.description.none.fl_txt_mv |
II International Symposium on Plum Pox Virus Sharka, caused by Plum pox virus (PPV), is the most important viral disease of stone fruits worldwide. PPV was detected for first time in Argentina in 2004 and since then many efforts have been made to eradicate it. To optimize sampling and virus detection by DAS-ELISA, 8 PPV-positive prune trees (P. domestica, cv. 'D´agen') were selected and sampled monthly from September 2008 to August 2010. The plant tissue collected was the one available at the sampling time (leaves, flowers or buds). The samples were taken from each of the four tree scaffolds at three different heights (basal, medium and apical). Variability of absorbance levels in each month was analyzed with Principal Components Analysis and sampling schemes were compared with non parametric variance analysis. This is the first study made in Argentina to tracking PPV relative virus concentration throughout the year. As described previously by many authors in the north hemisphere the highest levels of virus concentration, in both studied years, were found in spring (October and November) using flowers and young leaves. The scaffold basal height showed the highest PPV concentration in these two months. High variation of relative virus concentration levels were found among months, within and between years. Regarding different parts of the plant, relative virus concentration decreased from the base to the apical part of the scaffold, indicating that sampling should be performed at a basal height. This study will contribute to the optimization of DAS-ELISA PPV detection in Argentina. EEA Junín Fil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina Fil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina Fil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina Fil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina Fil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina Fil: Fuentes, C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Teich, Ingrid. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
II International Symposium on Plum Pox Virus |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01 2019-04-05T13:47:17Z 2019-04-05T13:47:17Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/4828 https://www.ishs.org/ishs-article/1063_25 0567-7572 2406-6168 (Online) https://doi.org/10.17660/ActaHortic.2015.1063.25 |
url |
http://hdl.handle.net/20.500.12123/4828 https://www.ishs.org/ishs-article/1063_25 https://doi.org/10.17660/ActaHortic.2015.1063.25 |
identifier_str_mv |
0567-7572 2406-6168 (Online) |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Society for Horticultural Science |
publisher.none.fl_str_mv |
International Society for Horticultural Science |
dc.source.none.fl_str_mv |
Acta horticulturae 1063 : 177-183. (January 2015) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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