Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications

Autores
Borsani, Julia; Budde, Claudio Olaf; Porrini, Lucía; Lauxmann, Martin Alexander; Lombardo, Verónica Andrea; Murray, Ricardo Ernesto; Andreo, Carlos Santiago; Drincovich, María Fabiana; Lara, María Valeria
Año de publicación
2009
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peach (Prunus persica L. Batsch) is a climacteric fruit that ripens after harvest, prior to human consumption. Organic acids and soluble sugars contribute to the overall organoleptic quality of fresh peach; thus, the integrated study of the metabolic pathways controlling the levels of these compounds is of great relevance. Therefore, in this work, several metabolites and enzymes involved in carbon metabolism were analysed during the post-harvest ripening of peach fruit cv ‘Dixiland’. Depending on the enzyme studied, activity, protein level by western blot, or transcript level by quantitative real time-PCR were analysed. Even though sorbitol did not accumulate at a high level in relation to sucrose at harvest, it was rapidly consumed once the fruit was separated from the tree. During the ripening process, sucrose degradation was accompanied by an increase of glucose and fructose. Specific transcripts encoding neutral invertases (NIs) were up-regulated or down-regulated, indicating differential functions for each putative NI isoform. Phosphoenolpyruvate carboxylase was markedly induced, and may participate as a glycolytic shunt, since the malate level did not increase during post-harvest ripening. The fermentative pathway was highly induced, with increases in both the acetaldehyde level and the enzymes involved in this process. In addition, proteins differentially expressed during the post-harvest ripening process were also analysed. Overall, the present study identified enzymes and pathways operating during the post-harvest ripening of peach fruit, which may contribute to further identification of varieties with altered levels of enzymes/metabolites or in the evaluation of post-harvest treatments to produce fruit of better organoleptic attributes.
EEA San Pedro
Fil: Bosani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Porrini, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lombardo, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Murray, Ricardo Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fuente
Journal of Experimental Botany 60 (6) : 1823-1837 (April 2009)
Materia
Durazno
Prunus persica
Ácidos Orgánicos
Maduramiento
Metabolismo
Maduración en Postcosecha
Fisiología Poscosecha
Peaches
Organic Acids
Ripening
Metabolism
Postharvest Ripening
Postharvest Physiology
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5323
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5323
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modificationsBorsani, JuliaBudde, Claudio OlafPorrini, LucíaLauxmann, Martin AlexanderLombardo, Verónica AndreaMurray, Ricardo ErnestoAndreo, Carlos SantiagoDrincovich, María FabianaLara, María ValeriaDuraznoPrunus persicaÁcidos OrgánicosMaduramientoMetabolismoMaduración en PostcosechaFisiología PoscosechaPeachesOrganic AcidsRipeningMetabolismPostharvest RipeningPostharvest PhysiologyPeach (Prunus persica L. Batsch) is a climacteric fruit that ripens after harvest, prior to human consumption. Organic acids and soluble sugars contribute to the overall organoleptic quality of fresh peach; thus, the integrated study of the metabolic pathways controlling the levels of these compounds is of great relevance. Therefore, in this work, several metabolites and enzymes involved in carbon metabolism were analysed during the post-harvest ripening of peach fruit cv ‘Dixiland’. Depending on the enzyme studied, activity, protein level by western blot, or transcript level by quantitative real time-PCR were analysed. Even though sorbitol did not accumulate at a high level in relation to sucrose at harvest, it was rapidly consumed once the fruit was separated from the tree. During the ripening process, sucrose degradation was accompanied by an increase of glucose and fructose. Specific transcripts encoding neutral invertases (NIs) were up-regulated or down-regulated, indicating differential functions for each putative NI isoform. Phosphoenolpyruvate carboxylase was markedly induced, and may participate as a glycolytic shunt, since the malate level did not increase during post-harvest ripening. The fermentative pathway was highly induced, with increases in both the acetaldehyde level and the enzymes involved in this process. In addition, proteins differentially expressed during the post-harvest ripening process were also analysed. Overall, the present study identified enzymes and pathways operating during the post-harvest ripening of peach fruit, which may contribute to further identification of varieties with altered levels of enzymes/metabolites or in the evaluation of post-harvest treatments to produce fruit of better organoleptic attributes.EEA San PedroFil: Bosani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Porrini, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Lombardo, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Murray, Ricardo Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaOxford Academic2019-06-14T14:26:10Z2019-06-14T14:26:10Z2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://academic.oup.com/jxb/article/60/6/1823/516686http://hdl.handle.net/20.500.12123/53231460-2431e0022-0957https://doi.org/10.1093/jxb/erp055Journal of Experimental Botany 60 (6) : 1823-1837 (April 2009)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:41Zoai:localhost:20.500.12123/5323instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:41.88INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
title Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
spellingShingle Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
Borsani, Julia
Durazno
Prunus persica
Ácidos Orgánicos
Maduramiento
Metabolismo
Maduración en Postcosecha
Fisiología Poscosecha
Peaches
Organic Acids
Ripening
Metabolism
Postharvest Ripening
Postharvest Physiology
title_short Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
title_full Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
title_fullStr Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
title_full_unstemmed Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
title_sort Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications
dc.creator.none.fl_str_mv Borsani, Julia
Budde, Claudio Olaf
Porrini, Lucía
Lauxmann, Martin Alexander
Lombardo, Verónica Andrea
Murray, Ricardo Ernesto
Andreo, Carlos Santiago
Drincovich, María Fabiana
Lara, María Valeria
author Borsani, Julia
author_facet Borsani, Julia
Budde, Claudio Olaf
Porrini, Lucía
Lauxmann, Martin Alexander
Lombardo, Verónica Andrea
Murray, Ricardo Ernesto
Andreo, Carlos Santiago
Drincovich, María Fabiana
Lara, María Valeria
author_role author
author2 Budde, Claudio Olaf
Porrini, Lucía
Lauxmann, Martin Alexander
Lombardo, Verónica Andrea
Murray, Ricardo Ernesto
Andreo, Carlos Santiago
Drincovich, María Fabiana
Lara, María Valeria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Durazno
Prunus persica
Ácidos Orgánicos
Maduramiento
Metabolismo
Maduración en Postcosecha
Fisiología Poscosecha
Peaches
Organic Acids
Ripening
Metabolism
Postharvest Ripening
Postharvest Physiology
topic Durazno
Prunus persica
Ácidos Orgánicos
Maduramiento
Metabolismo
Maduración en Postcosecha
Fisiología Poscosecha
Peaches
Organic Acids
Ripening
Metabolism
Postharvest Ripening
Postharvest Physiology
dc.description.none.fl_txt_mv Peach (Prunus persica L. Batsch) is a climacteric fruit that ripens after harvest, prior to human consumption. Organic acids and soluble sugars contribute to the overall organoleptic quality of fresh peach; thus, the integrated study of the metabolic pathways controlling the levels of these compounds is of great relevance. Therefore, in this work, several metabolites and enzymes involved in carbon metabolism were analysed during the post-harvest ripening of peach fruit cv ‘Dixiland’. Depending on the enzyme studied, activity, protein level by western blot, or transcript level by quantitative real time-PCR were analysed. Even though sorbitol did not accumulate at a high level in relation to sucrose at harvest, it was rapidly consumed once the fruit was separated from the tree. During the ripening process, sucrose degradation was accompanied by an increase of glucose and fructose. Specific transcripts encoding neutral invertases (NIs) were up-regulated or down-regulated, indicating differential functions for each putative NI isoform. Phosphoenolpyruvate carboxylase was markedly induced, and may participate as a glycolytic shunt, since the malate level did not increase during post-harvest ripening. The fermentative pathway was highly induced, with increases in both the acetaldehyde level and the enzymes involved in this process. In addition, proteins differentially expressed during the post-harvest ripening process were also analysed. Overall, the present study identified enzymes and pathways operating during the post-harvest ripening of peach fruit, which may contribute to further identification of varieties with altered levels of enzymes/metabolites or in the evaluation of post-harvest treatments to produce fruit of better organoleptic attributes.
EEA San Pedro
Fil: Bosani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Porrini, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lombardo, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Murray, Ricardo Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description Peach (Prunus persica L. Batsch) is a climacteric fruit that ripens after harvest, prior to human consumption. Organic acids and soluble sugars contribute to the overall organoleptic quality of fresh peach; thus, the integrated study of the metabolic pathways controlling the levels of these compounds is of great relevance. Therefore, in this work, several metabolites and enzymes involved in carbon metabolism were analysed during the post-harvest ripening of peach fruit cv ‘Dixiland’. Depending on the enzyme studied, activity, protein level by western blot, or transcript level by quantitative real time-PCR were analysed. Even though sorbitol did not accumulate at a high level in relation to sucrose at harvest, it was rapidly consumed once the fruit was separated from the tree. During the ripening process, sucrose degradation was accompanied by an increase of glucose and fructose. Specific transcripts encoding neutral invertases (NIs) were up-regulated or down-regulated, indicating differential functions for each putative NI isoform. Phosphoenolpyruvate carboxylase was markedly induced, and may participate as a glycolytic shunt, since the malate level did not increase during post-harvest ripening. The fermentative pathway was highly induced, with increases in both the acetaldehyde level and the enzymes involved in this process. In addition, proteins differentially expressed during the post-harvest ripening process were also analysed. Overall, the present study identified enzymes and pathways operating during the post-harvest ripening of peach fruit, which may contribute to further identification of varieties with altered levels of enzymes/metabolites or in the evaluation of post-harvest treatments to produce fruit of better organoleptic attributes.
publishDate 2009
dc.date.none.fl_str_mv 2009
2019-06-14T14:26:10Z
2019-06-14T14:26:10Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://academic.oup.com/jxb/article/60/6/1823/516686
http://hdl.handle.net/20.500.12123/5323
1460-2431e
0022-0957
https://doi.org/10.1093/jxb/erp055
url https://academic.oup.com/jxb/article/60/6/1823/516686
http://hdl.handle.net/20.500.12123/5323
https://doi.org/10.1093/jxb/erp055
identifier_str_mv 1460-2431e
0022-0957
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford Academic
publisher.none.fl_str_mv Oxford Academic
dc.source.none.fl_str_mv Journal of Experimental Botany 60 (6) : 1823-1837 (April 2009)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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