Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury

Autores
Bustamante, Claudia Anabel; Brotman, Yavid; Monti, Laura L.; Gabilondo, Julieta; Budde, Claudio Olaf; Lara, María Valeria; Fernie, Alisdair R.; Drincovich, María Fabiana
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peaches ripen and deteriorate rapidly at room temperature. Therefore, refrigeration is used to slow these processes and to extend fruit market life; however, many fruits develop chilling injury (CI) during storage at low temperature. Given that cell membranes are likely sites of the primary effects of chilling, the lipidome of six peach varieties with different susceptibility to CI was analyzed under different postharvest conditions. By using liquid chromatography coupled to mass spectrometry (LC–MS), 59 lipid species were detected, including diacyl- and triacylglycerides. The decreases in fruit firmness during postharvest ripening were accompanied by changes in the relative amount of several plastidic glycerolipid and triacylglyceride species, which may indicate their use as fuels prior to fruit senescence. In addition, levels of galactolipids were also modified in fruits stored at 0∘C for short and long periods, reflecting the stabilization of plastidic membranes at low temperature. When comparing susceptible and resistant varieties, the relative abundance of certain species of the lipid classes phosphatidylethanolamine, phosphatidylcholine and digalactosyldiacylglycerol correlated with the tolerance to CI, reflecting the importance of the plasma membrane in the development of CI symptoms and allowing the identification of possible lipid markers for chilling resistance. Finally, transcriptional analysis of genes involved in galactolipid metabolism revealed candidate genes responsible for the observed changes after cold exposure. When taken together, our results highlight the importance of plastids in the postharvest physiology of fruits and provide evidence that lipid composition and metabolism have a profound influence on the cold response.
EEA San Pedro
Fil: Bustamante, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Brotman, Yariv. Ben Gurion University of the Negev. Department of Life Sciences; Israel
Fil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Lara, Maria V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Drincovich, Maria Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fuente
Physiologia plantarum, vol. 163. 2018. p. 2-17
Materia
Durazno
Prunus persica
Almacenamiento en Frío
Fisiologia Postcosecha
Metabolismo de Lípidos
Daño por Frío
Variedades
Maduración en Postcosecha
Peaches
Cold Storage
Postharvest Physiology
Lipid Metabolism
Chilling Injury
Varieties
Postharvest Ripening
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2604
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oai_identifier_str oai:localhost:20.500.12123/2604
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network_name_str INTA Digital (INTA)
spelling Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injuryBustamante, Claudia AnabelBrotman, YavidMonti, Laura L.Gabilondo, JulietaBudde, Claudio OlafLara, María ValeriaFernie, Alisdair R.Drincovich, María FabianaDuraznoPrunus persicaAlmacenamiento en FríoFisiologia PostcosechaMetabolismo de LípidosDaño por FríoVariedadesMaduración en PostcosechaPeachesCold StoragePostharvest PhysiologyLipid MetabolismChilling InjuryVarietiesPostharvest RipeningPeaches ripen and deteriorate rapidly at room temperature. Therefore, refrigeration is used to slow these processes and to extend fruit market life; however, many fruits develop chilling injury (CI) during storage at low temperature. Given that cell membranes are likely sites of the primary effects of chilling, the lipidome of six peach varieties with different susceptibility to CI was analyzed under different postharvest conditions. By using liquid chromatography coupled to mass spectrometry (LC–MS), 59 lipid species were detected, including diacyl- and triacylglycerides. The decreases in fruit firmness during postharvest ripening were accompanied by changes in the relative amount of several plastidic glycerolipid and triacylglyceride species, which may indicate their use as fuels prior to fruit senescence. In addition, levels of galactolipids were also modified in fruits stored at 0∘C for short and long periods, reflecting the stabilization of plastidic membranes at low temperature. When comparing susceptible and resistant varieties, the relative abundance of certain species of the lipid classes phosphatidylethanolamine, phosphatidylcholine and digalactosyldiacylglycerol correlated with the tolerance to CI, reflecting the importance of the plasma membrane in the development of CI symptoms and allowing the identification of possible lipid markers for chilling resistance. Finally, transcriptional analysis of genes involved in galactolipid metabolism revealed candidate genes responsible for the observed changes after cold exposure. When taken together, our results highlight the importance of plastids in the postharvest physiology of fruits and provide evidence that lipid composition and metabolism have a profound influence on the cold response.EEA San PedroFil: Bustamante, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Brotman, Yariv. Ben Gurion University of the Negev. Department of Life Sciences; IsraelFil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Lara, Maria V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; AlemaniaFil: Drincovich, Maria Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina2018-06-12T15:31:06Z2018-06-12T15:31:06Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12665http://hdl.handle.net/20.500.12123/26041399-30540031-9317https://doi.org/10.1111/ppl.12665Physiologia plantarum, vol. 163. 2018. p. 2-17reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNFRU/1105083/AR./Nuevas tecnologías para el mantenimiento de la calidad en la cosecha, acondicionamiento y logística de frutas frescas.info:eu-repo/semantics/restrictedAccess2025-09-04T09:47:19Zoai:localhost:20.500.12123/2604instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:20.369INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
title Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
spellingShingle Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
Bustamante, Claudia Anabel
Durazno
Prunus persica
Almacenamiento en Frío
Fisiologia Postcosecha
Metabolismo de Lípidos
Daño por Frío
Variedades
Maduración en Postcosecha
Peaches
Cold Storage
Postharvest Physiology
Lipid Metabolism
Chilling Injury
Varieties
Postharvest Ripening
title_short Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
title_full Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
title_fullStr Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
title_full_unstemmed Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
title_sort Differential lipidome remodeling during postharvest of peach varieties with different susceptibility to chilling injury
dc.creator.none.fl_str_mv Bustamante, Claudia Anabel
Brotman, Yavid
Monti, Laura L.
Gabilondo, Julieta
Budde, Claudio Olaf
Lara, María Valeria
Fernie, Alisdair R.
Drincovich, María Fabiana
author Bustamante, Claudia Anabel
author_facet Bustamante, Claudia Anabel
Brotman, Yavid
Monti, Laura L.
Gabilondo, Julieta
Budde, Claudio Olaf
Lara, María Valeria
Fernie, Alisdair R.
Drincovich, María Fabiana
author_role author
author2 Brotman, Yavid
Monti, Laura L.
Gabilondo, Julieta
Budde, Claudio Olaf
Lara, María Valeria
Fernie, Alisdair R.
Drincovich, María Fabiana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Durazno
Prunus persica
Almacenamiento en Frío
Fisiologia Postcosecha
Metabolismo de Lípidos
Daño por Frío
Variedades
Maduración en Postcosecha
Peaches
Cold Storage
Postharvest Physiology
Lipid Metabolism
Chilling Injury
Varieties
Postharvest Ripening
topic Durazno
Prunus persica
Almacenamiento en Frío
Fisiologia Postcosecha
Metabolismo de Lípidos
Daño por Frío
Variedades
Maduración en Postcosecha
Peaches
Cold Storage
Postharvest Physiology
Lipid Metabolism
Chilling Injury
Varieties
Postharvest Ripening
dc.description.none.fl_txt_mv Peaches ripen and deteriorate rapidly at room temperature. Therefore, refrigeration is used to slow these processes and to extend fruit market life; however, many fruits develop chilling injury (CI) during storage at low temperature. Given that cell membranes are likely sites of the primary effects of chilling, the lipidome of six peach varieties with different susceptibility to CI was analyzed under different postharvest conditions. By using liquid chromatography coupled to mass spectrometry (LC–MS), 59 lipid species were detected, including diacyl- and triacylglycerides. The decreases in fruit firmness during postharvest ripening were accompanied by changes in the relative amount of several plastidic glycerolipid and triacylglyceride species, which may indicate their use as fuels prior to fruit senescence. In addition, levels of galactolipids were also modified in fruits stored at 0∘C for short and long periods, reflecting the stabilization of plastidic membranes at low temperature. When comparing susceptible and resistant varieties, the relative abundance of certain species of the lipid classes phosphatidylethanolamine, phosphatidylcholine and digalactosyldiacylglycerol correlated with the tolerance to CI, reflecting the importance of the plasma membrane in the development of CI symptoms and allowing the identification of possible lipid markers for chilling resistance. Finally, transcriptional analysis of genes involved in galactolipid metabolism revealed candidate genes responsible for the observed changes after cold exposure. When taken together, our results highlight the importance of plastids in the postharvest physiology of fruits and provide evidence that lipid composition and metabolism have a profound influence on the cold response.
EEA San Pedro
Fil: Bustamante, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Brotman, Yariv. Ben Gurion University of the Negev. Department of Life Sciences; Israel
Fil: Monti, Laura L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Lara, Maria V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Drincovich, Maria Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
description Peaches ripen and deteriorate rapidly at room temperature. Therefore, refrigeration is used to slow these processes and to extend fruit market life; however, many fruits develop chilling injury (CI) during storage at low temperature. Given that cell membranes are likely sites of the primary effects of chilling, the lipidome of six peach varieties with different susceptibility to CI was analyzed under different postharvest conditions. By using liquid chromatography coupled to mass spectrometry (LC–MS), 59 lipid species were detected, including diacyl- and triacylglycerides. The decreases in fruit firmness during postharvest ripening were accompanied by changes in the relative amount of several plastidic glycerolipid and triacylglyceride species, which may indicate their use as fuels prior to fruit senescence. In addition, levels of galactolipids were also modified in fruits stored at 0∘C for short and long periods, reflecting the stabilization of plastidic membranes at low temperature. When comparing susceptible and resistant varieties, the relative abundance of certain species of the lipid classes phosphatidylethanolamine, phosphatidylcholine and digalactosyldiacylglycerol correlated with the tolerance to CI, reflecting the importance of the plasma membrane in the development of CI symptoms and allowing the identification of possible lipid markers for chilling resistance. Finally, transcriptional analysis of genes involved in galactolipid metabolism revealed candidate genes responsible for the observed changes after cold exposure. When taken together, our results highlight the importance of plastids in the postharvest physiology of fruits and provide evidence that lipid composition and metabolism have a profound influence on the cold response.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-12T15:31:06Z
2018-06-12T15:31:06Z
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12665
http://hdl.handle.net/20.500.12123/2604
1399-3054
0031-9317
https://doi.org/10.1111/ppl.12665
url https://onlinelibrary.wiley.com/doi/abs/10.1111/ppl.12665
http://hdl.handle.net/20.500.12123/2604
https://doi.org/10.1111/ppl.12665
identifier_str_mv 1399-3054
0031-9317
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNFRU/1105083/AR./Nuevas tecnologías para el mantenimiento de la calidad en la cosecha, acondicionamiento y logística de frutas frescas.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Physiologia plantarum, vol. 163. 2018. p. 2-17
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.885934

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