In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol
- Autores
- Escoriaza, Maria Georgina; Garcia Lampasona, Sandra Claudia; Gomez Talquenca, Gonzalo; Piccoli, Patricia Noemí
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main terpene identified by gas chromatography electron impact mass spectrometry (GC-EIMS) was nerolidol, which was found in apical and basal tissues at a concentration of ca. 0.12 μg mg FW−1. Consistent with this, an increment in terpene synthase (TPS) activity, assessed as tritiated farnesyl pyrophosphate ([1−3H]-FPP) transformed into hexane-soluble radioactive products in infected plants, was observed. TPS activity increased in correlation with the fungal concentration. Nerolidol inhibited in vitro mycelium growth and reduced fungal growth, in a concentration dependent manner. According to our results, the response of grapevine tissues to Pm. parasiticum is systemic, as it can be visualized by an augment of transcript abundance of VvPNLinNer1. It is also characterized by an increase of de novo synthesis of TPS responsible for the biosynthesis of phytoalexin nerolidol.
EEA Mendoza
Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina - Fuente
- Plant cell, tissue and organ culture: PCTOC 138 (3) : 459–466. (September 2019)
- Materia
-
Vitis Vinífera
Vitroplantas
Fitoalexina
Respuesta Inmunológica
Infección
Enfermedades Fungosas
Terpenoidos
Vitroplants
Phytoalexins
Immune Response
Infection
Fungal Diseases
Terpenoids
Phaeoacremonium Parasiticum
Nerolidol
Terpene Synthase Activity - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/5679
Ver los metadatos del registro completo
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In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidolEscoriaza, Maria GeorginaGarcia Lampasona, Sandra ClaudiaGomez Talquenca, GonzaloPiccoli, Patricia NoemíVitis ViníferaVitroplantasFitoalexinaRespuesta InmunológicaInfecciónEnfermedades FungosasTerpenoidosVitroplantsPhytoalexinsImmune ResponseInfectionFungal DiseasesTerpenoidsPhaeoacremonium ParasiticumNerolidolTerpene Synthase ActivityThis study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main terpene identified by gas chromatography electron impact mass spectrometry (GC-EIMS) was nerolidol, which was found in apical and basal tissues at a concentration of ca. 0.12 μg mg FW−1. Consistent with this, an increment in terpene synthase (TPS) activity, assessed as tritiated farnesyl pyrophosphate ([1−3H]-FPP) transformed into hexane-soluble radioactive products in infected plants, was observed. TPS activity increased in correlation with the fungal concentration. Nerolidol inhibited in vitro mycelium growth and reduced fungal growth, in a concentration dependent manner. According to our results, the response of grapevine tissues to Pm. parasiticum is systemic, as it can be visualized by an augment of transcript abundance of VvPNLinNer1. It is also characterized by an increase of de novo synthesis of TPS responsible for the biosynthesis of phytoalexin nerolidol.EEA MendozaFil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaSpringer2019-08-23T13:06:25Z2019-08-23T13:06:25Z2019-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5679https://link.springer.com/article/10.1007%2Fs11240-019-01641-3Escoriaza, G., García Lampasona, S., Gomez Talquenca, S. et al. Plant Cell Tiss Organ Cult (2019) 138: 459. https://doi.org/10.1007/s11240-019-01641-30167-68571573-5044 (Online)https://doi.org/10.1007/s11240-019-01641-3Plant cell, tissue and organ culture: PCTOC 138 (3) : 459–466. (September 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:44Zoai:localhost:20.500.12123/5679instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:44.95INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title |
In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| spellingShingle |
In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol Escoriaza, Maria Georgina Vitis Vinífera Vitroplantas Fitoalexina Respuesta Inmunológica Infección Enfermedades Fungosas Terpenoidos Vitroplants Phytoalexins Immune Response Infection Fungal Diseases Terpenoids Phaeoacremonium Parasiticum Nerolidol Terpene Synthase Activity |
| title_short |
In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_full |
In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_fullStr |
In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_full_unstemmed |
In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| title_sort |
In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol |
| dc.creator.none.fl_str_mv |
Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí |
| author |
Escoriaza, Maria Georgina |
| author_facet |
Escoriaza, Maria Georgina Garcia Lampasona, Sandra Claudia Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí |
| author_role |
author |
| author2 |
Garcia Lampasona, Sandra Claudia Gomez Talquenca, Gonzalo Piccoli, Patricia Noemí |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Vitis Vinífera Vitroplantas Fitoalexina Respuesta Inmunológica Infección Enfermedades Fungosas Terpenoidos Vitroplants Phytoalexins Immune Response Infection Fungal Diseases Terpenoids Phaeoacremonium Parasiticum Nerolidol Terpene Synthase Activity |
| topic |
Vitis Vinífera Vitroplantas Fitoalexina Respuesta Inmunológica Infección Enfermedades Fungosas Terpenoidos Vitroplants Phytoalexins Immune Response Infection Fungal Diseases Terpenoids Phaeoacremonium Parasiticum Nerolidol Terpene Synthase Activity |
| dc.description.none.fl_txt_mv |
This study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main terpene identified by gas chromatography electron impact mass spectrometry (GC-EIMS) was nerolidol, which was found in apical and basal tissues at a concentration of ca. 0.12 μg mg FW−1. Consistent with this, an increment in terpene synthase (TPS) activity, assessed as tritiated farnesyl pyrophosphate ([1−3H]-FPP) transformed into hexane-soluble radioactive products in infected plants, was observed. TPS activity increased in correlation with the fungal concentration. Nerolidol inhibited in vitro mycelium growth and reduced fungal growth, in a concentration dependent manner. According to our results, the response of grapevine tissues to Pm. parasiticum is systemic, as it can be visualized by an augment of transcript abundance of VvPNLinNer1. It is also characterized by an increase of de novo synthesis of TPS responsible for the biosynthesis of phytoalexin nerolidol. EEA Mendoza Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina |
| description |
This study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main terpene identified by gas chromatography electron impact mass spectrometry (GC-EIMS) was nerolidol, which was found in apical and basal tissues at a concentration of ca. 0.12 μg mg FW−1. Consistent with this, an increment in terpene synthase (TPS) activity, assessed as tritiated farnesyl pyrophosphate ([1−3H]-FPP) transformed into hexane-soluble radioactive products in infected plants, was observed. TPS activity increased in correlation with the fungal concentration. Nerolidol inhibited in vitro mycelium growth and reduced fungal growth, in a concentration dependent manner. According to our results, the response of grapevine tissues to Pm. parasiticum is systemic, as it can be visualized by an augment of transcript abundance of VvPNLinNer1. It is also characterized by an increase of de novo synthesis of TPS responsible for the biosynthesis of phytoalexin nerolidol. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-08-23T13:06:25Z 2019-08-23T13:06:25Z 2019-09 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/5679 https://link.springer.com/article/10.1007%2Fs11240-019-01641-3 Escoriaza, G., García Lampasona, S., Gomez Talquenca, S. et al. Plant Cell Tiss Organ Cult (2019) 138: 459. https://doi.org/10.1007/s11240-019-01641-3 0167-6857 1573-5044 (Online) https://doi.org/10.1007/s11240-019-01641-3 |
| url |
http://hdl.handle.net/20.500.12123/5679 https://link.springer.com/article/10.1007%2Fs11240-019-01641-3 https://doi.org/10.1007/s11240-019-01641-3 |
| identifier_str_mv |
Escoriaza, G., García Lampasona, S., Gomez Talquenca, S. et al. Plant Cell Tiss Organ Cult (2019) 138: 459. https://doi.org/10.1007/s11240-019-01641-3 0167-6857 1573-5044 (Online) |
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eng |
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eng |
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application/pdf |
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Springer |
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Springer |
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Plant cell, tissue and organ culture: PCTOC 138 (3) : 459–466. (September 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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