In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum

• Autores: Escoriaza, María Georgina; Sansberro, Pedro Alfonso; Garcia Lampasona, Sandra Claudia; Gatica, Marta; Bottini, Rubén; Piccoli, Patricia Noemi
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: GRAPEVINE; GRAPEVINE TRUNK DISEASES; TERPENE; TERPENE SYNTHASE ACTIVITY
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

This study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of the fungi associated with the grapevine diseases known as “hoja de malvón” and young vine decline. The highest TPS activity, assessed as tritiated farnesyl pyrophosphate ([1-3H]-FPP) transformed into hexane-soluble radioactive products, was observed in both inoculated calluses and cell suspension cultures (CSC). When tested in inoculated cell suspension cultures the TPS activity was maximal at 8 h after [1-3H]-FPP application and then declined; this was associated with a temporary increase of the sesquiterpene nerolidol. Grape calluses produced: α-pinene, nerolidol and squalene whether or not they were inoculated with Pm. parasiticum. As fungal amount raised the relative concentration of α-pinene and nerolidol increased in respect to squalene in calluses. The TPS activity and nerolidol and α-pinene accumulation was correlated with the increase in the amount of inoculated fungus. Of the mentioned metabolites mainly squalene was identified from extracts of fungal cultures. The results suggest that the response of grapevine tissues to Pm. parasiticum is dependent on the pathogen concentration and is characterized by increasing TPS activity through de novo synthesis.
Fil: Escoriaza, María Georgina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza - San Juan. Estación Experimental Agropecuaria Mendoza; Argentina;
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina;
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina; Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina;
Fil: Gatica, Marta. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza - San Juan. Estación Experimental Agropecuaria Mendoza; Argentina;
Fil: Bottini, Rubén. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Universidad Nacional de Cuyo; Argentina;
Fil: Piccoli, Patricia Noemi. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto de Biologia Agricola de Mendoza; Argentina; Universidad Nacional de Cuyo; Argentina;