In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum

Autores
Escoriaza, Maria Georgina; Sansberro, Pedro; Garcia Lampasona, Sandra Claudia; Gatica, Marta Elcira; Bottini, Ambrosio Rubén; Piccoli, Patricia Noemí
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of the fungi associated with the grapevine diseases known as “hoja de malvón” and young vine decline. The highest TPS activity, assessed as tritiated farnesyl pyrophosphate ([1-3H]-FPP) transformed into hexane-soluble radioactive products, was observed in both inoculated calluses and cell suspension cultures (CSC). When tested in inoculated cell suspension cultures the TPS activity was maximal at 8 h after [1-3H]-FPP application and then declined; this was associated with a temporary increase of the sesquiterpene nerolidol. Grape calluses produced: α-pinene, nerolidol and squalene whether or not they were inoculated with Pm. parasiticum. As fungal amount raised the relative concentration of α-pinene and nerolidol increased in respect to squalene in calluses. The TPS activity and nerolidol and α-pinene accumulation was correlated with the increase in the amount of inoculated fungus. Of the mentioned metabolites mainly squalene was identified from extracts of fungal cultures. The results suggest that the response of grapevine tissues to Pm. parasiticum is dependent on the pathogen concentration and is characterized by increasing TPS activity through de novo synthesis.
Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología; Argentina
Fil: Sansberro, Pedro. Universidad Nacional del Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas.Instituto de Botánica del Nordeste; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biología Molecular; Argentina
Fil: Gatica, Marta Elcira. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología; Argentina
Fil: Bottini, Rubén. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina
Fuente
Phytopathologia Mediterranea 52 (2) : 289−297 (2013)
Materia
Vitis Vinifera
Vid
Variedades
Grapevines
Varieties
In Vitro Experimentation
Phytoalexins
Terpenoids
Experimentación In Vitro
Fitoalexina
Terpenoidos
Chardonnay
Phaeoacremonium Parasiticum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1650
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1650
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticumEscoriaza, Maria GeorginaSansberro, PedroGarcia Lampasona, Sandra ClaudiaGatica, Marta ElciraBottini, Ambrosio RubénPiccoli, Patricia NoemíVitis ViniferaVidVariedadesGrapevinesVarietiesIn Vitro ExperimentationPhytoalexinsTerpenoidsExperimentación In VitroFitoalexinaTerpenoidosChardonnayPhaeoacremonium ParasiticumThis study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of the fungi associated with the grapevine diseases known as “hoja de malvón” and young vine decline. The highest TPS activity, assessed as tritiated farnesyl pyrophosphate ([1-3H]-FPP) transformed into hexane-soluble radioactive products, was observed in both inoculated calluses and cell suspension cultures (CSC). When tested in inoculated cell suspension cultures the TPS activity was maximal at 8 h after [1-3H]-FPP application and then declined; this was associated with a temporary increase of the sesquiterpene nerolidol. Grape calluses produced: α-pinene, nerolidol and squalene whether or not they were inoculated with Pm. parasiticum. As fungal amount raised the relative concentration of α-pinene and nerolidol increased in respect to squalene in calluses. The TPS activity and nerolidol and α-pinene accumulation was correlated with the increase in the amount of inoculated fungus. Of the mentioned metabolites mainly squalene was identified from extracts of fungal cultures. The results suggest that the response of grapevine tissues to Pm. parasiticum is dependent on the pathogen concentration and is characterized by increasing TPS activity through de novo synthesis.Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología; ArgentinaFil: Sansberro, Pedro. Universidad Nacional del Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas.Instituto de Botánica del Nordeste; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biología Molecular; ArgentinaFil: Gatica, Marta Elcira. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología; ArgentinaFil: Bottini, Rubén. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Piccoli, Patricia Noemí. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina2017-11-01T15:33:12Z2017-11-01T15:33:12Z2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1650https://oajournals.fupress.net/index.php/pm/article/view/55200031-9465 (Print)1593-2095 (Online)Phytopathologia Mediterranea 52 (2) : 289−297 (2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:13Zoai:localhost:20.500.12123/1650instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:13.467INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
title In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
spellingShingle In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
Escoriaza, Maria Georgina
Vitis Vinifera
Vid
Variedades
Grapevines
Varieties
In Vitro Experimentation
Phytoalexins
Terpenoids
Experimentación In Vitro
Fitoalexina
Terpenoidos
Chardonnay
Phaeoacremonium Parasiticum
title_short In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
title_full In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
title_fullStr In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
title_full_unstemmed In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
title_sort In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum
dc.creator.none.fl_str_mv Escoriaza, Maria Georgina
Sansberro, Pedro
Garcia Lampasona, Sandra Claudia
Gatica, Marta Elcira
Bottini, Ambrosio Rubén
Piccoli, Patricia Noemí
author Escoriaza, Maria Georgina
author_facet Escoriaza, Maria Georgina
Sansberro, Pedro
Garcia Lampasona, Sandra Claudia
Gatica, Marta Elcira
Bottini, Ambrosio Rubén
Piccoli, Patricia Noemí
author_role author
author2 Sansberro, Pedro
Garcia Lampasona, Sandra Claudia
Gatica, Marta Elcira
Bottini, Ambrosio Rubén
Piccoli, Patricia Noemí
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Vitis Vinifera
Vid
Variedades
Grapevines
Varieties
In Vitro Experimentation
Phytoalexins
Terpenoids
Experimentación In Vitro
Fitoalexina
Terpenoidos
Chardonnay
Phaeoacremonium Parasiticum
topic Vitis Vinifera
Vid
Variedades
Grapevines
Varieties
In Vitro Experimentation
Phytoalexins
Terpenoids
Experimentación In Vitro
Fitoalexina
Terpenoidos
Chardonnay
Phaeoacremonium Parasiticum
dc.description.none.fl_txt_mv This study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of the fungi associated with the grapevine diseases known as “hoja de malvón” and young vine decline. The highest TPS activity, assessed as tritiated farnesyl pyrophosphate ([1-3H]-FPP) transformed into hexane-soluble radioactive products, was observed in both inoculated calluses and cell suspension cultures (CSC). When tested in inoculated cell suspension cultures the TPS activity was maximal at 8 h after [1-3H]-FPP application and then declined; this was associated with a temporary increase of the sesquiterpene nerolidol. Grape calluses produced: α-pinene, nerolidol and squalene whether or not they were inoculated with Pm. parasiticum. As fungal amount raised the relative concentration of α-pinene and nerolidol increased in respect to squalene in calluses. The TPS activity and nerolidol and α-pinene accumulation was correlated with the increase in the amount of inoculated fungus. Of the mentioned metabolites mainly squalene was identified from extracts of fungal cultures. The results suggest that the response of grapevine tissues to Pm. parasiticum is dependent on the pathogen concentration and is characterized by increasing TPS activity through de novo synthesis.
Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología; Argentina
Fil: Sansberro, Pedro. Universidad Nacional del Nordeste-Consejo Nacional de Investigaciones Científicas y Técnicas.Instituto de Botánica del Nordeste; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biología Molecular; Argentina
Fil: Gatica, Marta Elcira. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología; Argentina
Fil: Bottini, Rubén. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Universidad Nacional de Cuyo-Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina
description This study investigated terpene synthase (TPS) activity and terpene antifungal metabolites in calluses and cell suspension cultures of Vitis vinifera cv. Chardonnay infected with Phaecremonium parasiticum, one of the fungi associated with the grapevine diseases known as “hoja de malvón” and young vine decline. The highest TPS activity, assessed as tritiated farnesyl pyrophosphate ([1-3H]-FPP) transformed into hexane-soluble radioactive products, was observed in both inoculated calluses and cell suspension cultures (CSC). When tested in inoculated cell suspension cultures the TPS activity was maximal at 8 h after [1-3H]-FPP application and then declined; this was associated with a temporary increase of the sesquiterpene nerolidol. Grape calluses produced: α-pinene, nerolidol and squalene whether or not they were inoculated with Pm. parasiticum. As fungal amount raised the relative concentration of α-pinene and nerolidol increased in respect to squalene in calluses. The TPS activity and nerolidol and α-pinene accumulation was correlated with the increase in the amount of inoculated fungus. Of the mentioned metabolites mainly squalene was identified from extracts of fungal cultures. The results suggest that the response of grapevine tissues to Pm. parasiticum is dependent on the pathogen concentration and is characterized by increasing TPS activity through de novo synthesis.
publishDate 2013
dc.date.none.fl_str_mv 2013
2017-11-01T15:33:12Z
2017-11-01T15:33:12Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1650
https://oajournals.fupress.net/index.php/pm/article/view/5520
0031-9465 (Print)
1593-2095 (Online)
url http://hdl.handle.net/20.500.12123/1650
https://oajournals.fupress.net/index.php/pm/article/view/5520
identifier_str_mv 0031-9465 (Print)
1593-2095 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Phytopathologia Mediterranea 52 (2) : 289−297 (2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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