Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage

Autores
Perotti, Valeria Elisa; Moreno, Alejandra Soledad; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo Enrique; Bello, Fernando; Cocco, Mariángeles; Vazquez, Daniel Eduardo; Podesta, Florencio Esteban
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit dur-ing the postharvest. Several studies have been dedicated to investigate the general chemical changes thatjustify the immediate reaction responsible for the induced tolerance; but just how primary metabolismand enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. Inthis work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during thepostharvest period after a heat treatment (HT) of 48 h at 37◦C. Enzymatic activity measurements indi-cated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylatingglyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthe-sis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would havean important role in sucrose accumulation. The pathway of carbon through glycolysis was affected bycold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase overthe PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxy-lase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes inresponse to HT, although some of them showed a marked descent along the cold storage. Proteomic stud-ies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and smallHSPs, completing the description of the main metabolic changes in this tissue.
EEA Concordia
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Moreno, Alejandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Meier, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fuente
Postharvest biology and technology 99 : 80–87. (2015)
Materia
Frutas Cítricas
Naranja Dulce
Glicólisis
Almacenamiento en Frío
Tratamiento Térmico
Tecnología Postcosecha
Postharvest Technology
Heat Treatment
Cold Storage
Glycolysis
Sweet Oranges
Citrus Fruits
Proteomics
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2829
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2829
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storagePerotti, Valeria ElisaMoreno, Alejandra SoledadTripodi, Karina Eva Josefinadel Vecchio, Hernán A.Meier, Guillermo EnriqueBello, FernandoCocco, MariángelesVazquez, Daniel EduardoPodesta, Florencio EstebanFrutas CítricasNaranja DulceGlicólisisAlmacenamiento en FríoTratamiento TérmicoTecnología PostcosechaPostharvest TechnologyHeat TreatmentCold StorageGlycolysisSweet OrangesCitrus FruitsProteomicsHeat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit dur-ing the postharvest. Several studies have been dedicated to investigate the general chemical changes thatjustify the immediate reaction responsible for the induced tolerance; but just how primary metabolismand enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. Inthis work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during thepostharvest period after a heat treatment (HT) of 48 h at 37◦C. Enzymatic activity measurements indi-cated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylatingglyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthe-sis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would havean important role in sucrose accumulation. The pathway of carbon through glycolysis was affected bycold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase overthe PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxy-lase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes inresponse to HT, although some of them showed a marked descent along the cold storage. Proteomic stud-ies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and smallHSPs, completing the description of the main metabolic changes in this tissue.EEA ConcordiaFil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Moreno, Alejandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Meier, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina2018-07-19T15:16:52Z2018-07-19T15:16:52Z2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2829https://www.sciencedirect.com/science/article/pii/S09255214140024150925-5214https://doi.org/10.1016/j.pmpp.2016.05.001Postharvest biology and technology 99 : 80–87. (2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:21Zoai:localhost:20.500.12123/2829instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:22.29INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
spellingShingle Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
Perotti, Valeria Elisa
Frutas Cítricas
Naranja Dulce
Glicólisis
Almacenamiento en Frío
Tratamiento Térmico
Tecnología Postcosecha
Postharvest Technology
Heat Treatment
Cold Storage
Glycolysis
Sweet Oranges
Citrus Fruits
Proteomics
title_short Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title_full Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title_fullStr Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title_full_unstemmed Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title_sort Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
dc.creator.none.fl_str_mv Perotti, Valeria Elisa
Moreno, Alejandra Soledad
Tripodi, Karina Eva Josefina
del Vecchio, Hernán A.
Meier, Guillermo Enrique
Bello, Fernando
Cocco, Mariángeles
Vazquez, Daniel Eduardo
Podesta, Florencio Esteban
author Perotti, Valeria Elisa
author_facet Perotti, Valeria Elisa
Moreno, Alejandra Soledad
Tripodi, Karina Eva Josefina
del Vecchio, Hernán A.
Meier, Guillermo Enrique
Bello, Fernando
Cocco, Mariángeles
Vazquez, Daniel Eduardo
Podesta, Florencio Esteban
author_role author
author2 Moreno, Alejandra Soledad
Tripodi, Karina Eva Josefina
del Vecchio, Hernán A.
Meier, Guillermo Enrique
Bello, Fernando
Cocco, Mariángeles
Vazquez, Daniel Eduardo
Podesta, Florencio Esteban
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Frutas Cítricas
Naranja Dulce
Glicólisis
Almacenamiento en Frío
Tratamiento Térmico
Tecnología Postcosecha
Postharvest Technology
Heat Treatment
Cold Storage
Glycolysis
Sweet Oranges
Citrus Fruits
Proteomics
topic Frutas Cítricas
Naranja Dulce
Glicólisis
Almacenamiento en Frío
Tratamiento Térmico
Tecnología Postcosecha
Postharvest Technology
Heat Treatment
Cold Storage
Glycolysis
Sweet Oranges
Citrus Fruits
Proteomics
dc.description.none.fl_txt_mv Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit dur-ing the postharvest. Several studies have been dedicated to investigate the general chemical changes thatjustify the immediate reaction responsible for the induced tolerance; but just how primary metabolismand enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. Inthis work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during thepostharvest period after a heat treatment (HT) of 48 h at 37◦C. Enzymatic activity measurements indi-cated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylatingglyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthe-sis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would havean important role in sucrose accumulation. The pathway of carbon through glycolysis was affected bycold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase overthe PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxy-lase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes inresponse to HT, although some of them showed a marked descent along the cold storage. Proteomic stud-ies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and smallHSPs, completing the description of the main metabolic changes in this tissue.
EEA Concordia
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Moreno, Alejandra Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Meier, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit dur-ing the postharvest. Several studies have been dedicated to investigate the general chemical changes thatjustify the immediate reaction responsible for the induced tolerance; but just how primary metabolismand enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. Inthis work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during thepostharvest period after a heat treatment (HT) of 48 h at 37◦C. Enzymatic activity measurements indi-cated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylatingglyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthe-sis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would havean important role in sucrose accumulation. The pathway of carbon through glycolysis was affected bycold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase overthe PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxy-lase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes inresponse to HT, although some of them showed a marked descent along the cold storage. Proteomic stud-ies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and smallHSPs, completing the description of the main metabolic changes in this tissue.
publishDate 2015
dc.date.none.fl_str_mv 2015
2018-07-19T15:16:52Z
2018-07-19T15:16:52Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2829
https://www.sciencedirect.com/science/article/pii/S0925521414002415
0925-5214
https://doi.org/10.1016/j.pmpp.2016.05.001
url http://hdl.handle.net/20.500.12123/2829
https://www.sciencedirect.com/science/article/pii/S0925521414002415
https://doi.org/10.1016/j.pmpp.2016.05.001
identifier_str_mv 0925-5214
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Postharvest biology and technology 99 : 80–87. (2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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