Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure

Autores
Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; Meier, Guillermo; Bello, Fernando; Cocco, Mariángeles; Vázquez, Daniel; Anderson, Catalina; Podesta, Florencio Esteban
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost-exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold-sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Meier, Guillermo. Estacion Extal. Agrop. Concordia; Argentina
Fil: Bello, Fernando. Estacion Extal. Agrop. Concordia; Argentina
Fil: Cocco, Mariángeles. Estacion Extal. Agrop. Concordia; Argentina
Fil: Vázquez, Daniel. Estacion Extal. Agrop. Concordia; Argentina
Fil: Anderson, Catalina. Estacion Extal. Agrop. Concordia; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Materia
Citrus
Proteomics
Glycolysis
Metabolomics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7861
Acceder
id CONICETDig_3652157c00480c382b471e687d8bc51d
oai_identifier_str oai:ri.conicet.gov.ar:11336/7861
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposurePerotti, Valeria ElisaMoreno, Alejandra S.Tripodi, Karina Eva JosefinaMeier, GuillermoBello, FernandoCocco, MariángelesVázquez, DanielAnderson, CatalinaPodesta, Florencio EstebanCitrusProteomicsGlycolysisMetabolomicshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost-exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold-sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaFil: Meier, Guillermo. Estacion Extal. Agrop. Concordia; ArgentinaFil: Bello, Fernando. Estacion Extal. Agrop. Concordia; ArgentinaFil: Cocco, Mariángeles. Estacion Extal. Agrop. Concordia; ArgentinaFil: Vázquez, Daniel. Estacion Extal. Agrop. Concordia; ArgentinaFil: Anderson, Catalina. Estacion Extal. Agrop. Concordia; ArgentinaFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); ArgentinaWiley2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7861Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; Meier, Guillermo; Bello, Fernando; et al.; Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure; Wiley; Physiologia Plantarum; 153; 3; 5-2014; 337–3540031-9317enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ppl.12259/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/ppl.12259info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:10Zoai:ri.conicet.gov.ar:11336/7861instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:11.13CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
title Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
spellingShingle Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
Perotti, Valeria Elisa
Citrus
Proteomics
Glycolysis
Metabolomics
title_short Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
title_full Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
title_fullStr Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
title_full_unstemmed Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
title_sort Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
dc.creator.none.fl_str_mv Perotti, Valeria Elisa
Moreno, Alejandra S.
Tripodi, Karina Eva Josefina
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Vázquez, Daniel
Anderson, Catalina
Podesta, Florencio Esteban
author Perotti, Valeria Elisa
author_facet Perotti, Valeria Elisa
Moreno, Alejandra S.
Tripodi, Karina Eva Josefina
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Vázquez, Daniel
Anderson, Catalina
Podesta, Florencio Esteban
author_role author
author2 Moreno, Alejandra S.
Tripodi, Karina Eva Josefina
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Vázquez, Daniel
Anderson, Catalina
Podesta, Florencio Esteban
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citrus
Proteomics
Glycolysis
Metabolomics
topic Citrus
Proteomics
Glycolysis
Metabolomics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost-exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold-sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
Fil: Meier, Guillermo. Estacion Extal. Agrop. Concordia; Argentina
Fil: Bello, Fernando. Estacion Extal. Agrop. Concordia; Argentina
Fil: Cocco, Mariángeles. Estacion Extal. Agrop. Concordia; Argentina
Fil: Vázquez, Daniel. Estacion Extal. Agrop. Concordia; Argentina
Fil: Anderson, Catalina. Estacion Extal. Agrop. Concordia; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina
description The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost-exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold-sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7861
Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; Meier, Guillermo; Bello, Fernando; et al.; Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure; Wiley; Physiologia Plantarum; 153; 3; 5-2014; 337–354
0031-9317
url http://hdl.handle.net/11336/7861
identifier_str_mv Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; Meier, Guillermo; Bello, Fernando; et al.; Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure; Wiley; Physiologia Plantarum; 153; 3; 5-2014; 337–354
0031-9317
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ppl.12259/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/ppl.12259
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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