Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage

Autores
Perotti, Valeria Elisa; del Vecchio, Hernán A.; Sansevich, Alina; Meier, Guillermo; Bello, Fernando; Cocco, Mariángeles; Garrán, Sergio M.; Anderson, Catalina; Vázquez, Daniel; Podesta, Florencio Esteban
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected to heat treatment before 0, 30 and 60 d of storage at 5 °C. Heat treatment for 48 h at 37 °C and 90% relative humidity was followed by 24 h at 20 °C. Differentially expressed proteins were detected by two-dimensional differential gel electrophoresis and identified by matrix-assisted laser desorption/ionization MS. Twenty eight different proteins in 40 spots among the 61 analyzed were identified. Functional classification showed that the main affected categories were “Cell rescue, defense, and virulence” and “Metabolism”; while 20% were found to be storage proteins with the rest related to minor categories. Activity of antioxidant enzymes was also evaluated. The most conspicuous change was an increase in superoxide dismutase in both the flavedo and juice sacs. Peroxidases increased in flavedo but decreased in sacs while alcohol dehydrogenase increased in treated flavedo, but not in sacs. Other fermentative enzymes were not affected. Metabolite content showed a higher concentration of sugars in heat treated fruit, while the main acids were slightly or not affected. These results are consistent with the development of a lower degree of fruit susceptibility to fungal pathogens, thus explaining the maintenance of postharvest quality.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Sansevich, Alina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Garrán, Sergio M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Anderson, Catalina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Materia
Citrus Fruit
Fermentative Metabolism
Fruit Biochemistry
Orange Postharvest Proteomics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15451
Acceder
id CONICETDig_b9acc564d2da38416fde42491f86241d
oai_identifier_str oai:ri.conicet.gov.ar:11336/15451
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storagePerotti, Valeria Elisadel Vecchio, Hernán A.Sansevich, AlinaMeier, GuillermoBello, FernandoCocco, MariángelesGarrán, Sergio M.Anderson, CatalinaVázquez, DanielPodesta, Florencio EstebanCitrus FruitFermentative MetabolismFruit BiochemistryOrange Postharvest Proteomicshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected to heat treatment before 0, 30 and 60 d of storage at 5 °C. Heat treatment for 48 h at 37 °C and 90% relative humidity was followed by 24 h at 20 °C. Differentially expressed proteins were detected by two-dimensional differential gel electrophoresis and identified by matrix-assisted laser desorption/ionization MS. Twenty eight different proteins in 40 spots among the 61 analyzed were identified. Functional classification showed that the main affected categories were “Cell rescue, defense, and virulence” and “Metabolism”; while 20% were found to be storage proteins with the rest related to minor categories. Activity of antioxidant enzymes was also evaluated. The most conspicuous change was an increase in superoxide dismutase in both the flavedo and juice sacs. Peroxidases increased in flavedo but decreased in sacs while alcohol dehydrogenase increased in treated flavedo, but not in sacs. Other fermentative enzymes were not affected. Metabolite content showed a higher concentration of sugars in heat treated fruit, while the main acids were slightly or not affected. These results are consistent with the development of a lower degree of fruit susceptibility to fungal pathogens, thus explaining the maintenance of postharvest quality.Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Sansevich, Alina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Garrán, Sergio M.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Anderson, Catalina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaElsevier Science2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15451Perotti, Valeria Elisa; del Vecchio, Hernán A.; Sansevich, Alina; Meier, Guillermo; Bello, Fernando; et al.; Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage; Elsevier Science; Postharvest Biology And Technology; 62; 2; 11-2011; 97-1140925-5214enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2011.05.015info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925521411001323info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:56Zoai:ri.conicet.gov.ar:11336/15451instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:57.025CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
title Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
spellingShingle Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
Perotti, Valeria Elisa
Citrus Fruit
Fermentative Metabolism
Fruit Biochemistry
Orange Postharvest Proteomics
title_short Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
title_full Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
title_fullStr Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
title_full_unstemmed Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
title_sort Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage
dc.creator.none.fl_str_mv Perotti, Valeria Elisa
del Vecchio, Hernán A.
Sansevich, Alina
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Garrán, Sergio M.
Anderson, Catalina
Vázquez, Daniel
Podesta, Florencio Esteban
author Perotti, Valeria Elisa
author_facet Perotti, Valeria Elisa
del Vecchio, Hernán A.
Sansevich, Alina
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Garrán, Sergio M.
Anderson, Catalina
Vázquez, Daniel
Podesta, Florencio Esteban
author_role author
author2 del Vecchio, Hernán A.
Sansevich, Alina
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Garrán, Sergio M.
Anderson, Catalina
Vázquez, Daniel
Podesta, Florencio Esteban
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citrus Fruit
Fermentative Metabolism
Fruit Biochemistry
Orange Postharvest Proteomics
topic Citrus Fruit
Fermentative Metabolism
Fruit Biochemistry
Orange Postharvest Proteomics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected to heat treatment before 0, 30 and 60 d of storage at 5 °C. Heat treatment for 48 h at 37 °C and 90% relative humidity was followed by 24 h at 20 °C. Differentially expressed proteins were detected by two-dimensional differential gel electrophoresis and identified by matrix-assisted laser desorption/ionization MS. Twenty eight different proteins in 40 spots among the 61 analyzed were identified. Functional classification showed that the main affected categories were “Cell rescue, defense, and virulence” and “Metabolism”; while 20% were found to be storage proteins with the rest related to minor categories. Activity of antioxidant enzymes was also evaluated. The most conspicuous change was an increase in superoxide dismutase in both the flavedo and juice sacs. Peroxidases increased in flavedo but decreased in sacs while alcohol dehydrogenase increased in treated flavedo, but not in sacs. Other fermentative enzymes were not affected. Metabolite content showed a higher concentration of sugars in heat treated fruit, while the main acids were slightly or not affected. These results are consistent with the development of a lower degree of fruit susceptibility to fungal pathogens, thus explaining the maintenance of postharvest quality.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Sansevich, Alina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Garrán, Sergio M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Anderson, Catalina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description Heat treatment induces defense mechanisms and triggers physiological responses that allow citrus fruit to withstand stressful conditions during storage. The aim of this paper was to identify changes in the proteome and the biochemistry of Valencia oranges (Citrus sinensis cv Valencia late) subjected to heat treatment before 0, 30 and 60 d of storage at 5 °C. Heat treatment for 48 h at 37 °C and 90% relative humidity was followed by 24 h at 20 °C. Differentially expressed proteins were detected by two-dimensional differential gel electrophoresis and identified by matrix-assisted laser desorption/ionization MS. Twenty eight different proteins in 40 spots among the 61 analyzed were identified. Functional classification showed that the main affected categories were “Cell rescue, defense, and virulence” and “Metabolism”; while 20% were found to be storage proteins with the rest related to minor categories. Activity of antioxidant enzymes was also evaluated. The most conspicuous change was an increase in superoxide dismutase in both the flavedo and juice sacs. Peroxidases increased in flavedo but decreased in sacs while alcohol dehydrogenase increased in treated flavedo, but not in sacs. Other fermentative enzymes were not affected. Metabolite content showed a higher concentration of sugars in heat treated fruit, while the main acids were slightly or not affected. These results are consistent with the development of a lower degree of fruit susceptibility to fungal pathogens, thus explaining the maintenance of postharvest quality.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15451
Perotti, Valeria Elisa; del Vecchio, Hernán A.; Sansevich, Alina; Meier, Guillermo; Bello, Fernando; et al.; Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage; Elsevier Science; Postharvest Biology And Technology; 62; 2; 11-2011; 97-114
0925-5214
url http://hdl.handle.net/11336/15451
identifier_str_mv Perotti, Valeria Elisa; del Vecchio, Hernán A.; Sansevich, Alina; Meier, Guillermo; Bello, Fernando; et al.; Proteomic, metabalomic, and biochemical analysis of heat treated Valencia oranges during storage; Elsevier Science; Postharvest Biology And Technology; 62; 2; 11-2011; 97-114
0925-5214
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2011.05.015
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925521411001323
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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