Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage

Autores
Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo; Bello, Fernando; Cocco, Mariángeles; Vázquez, Daniel; Podesta, Florencio Esteban
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Materia
Citrus
Proteomics
Metabolomics
Postharvest
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15446
Acceder
id CONICETDig_fd51a1c2db3ef79b4bd57ac9c49b7687
oai_identifier_str oai:ri.conicet.gov.ar:11336/15446
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storagePerotti, Valeria ElisaMoreno, Alejandra S.Tripodi, Karina Eva Josefinadel Vecchio, Hernán A.Meier, GuillermoBello, FernandoCocco, MariángelesVázquez, DanielPodesta, Florencio EstebanCitrusProteomicsMetabolomicsPostharvesthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue.Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaElsevier Science2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15446Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo; et al.; Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage; Elsevier Science; Postharvest Biology And Technology; 99; 1-2015; 80-870925-5214enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2014.08.007info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925521414002415info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:49Zoai:ri.conicet.gov.ar:11336/15446instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:49.548CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
spellingShingle Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
Perotti, Valeria Elisa
Citrus
Proteomics
Metabolomics
Postharvest
title_short Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title_full Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title_fullStr Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title_full_unstemmed Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
title_sort Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
dc.creator.none.fl_str_mv Perotti, Valeria Elisa
Moreno, Alejandra S.
Tripodi, Karina Eva Josefina
del Vecchio, Hernán A.
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Vázquez, Daniel
Podesta, Florencio Esteban
author Perotti, Valeria Elisa
author_facet Perotti, Valeria Elisa
Moreno, Alejandra S.
Tripodi, Karina Eva Josefina
del Vecchio, Hernán A.
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Vázquez, Daniel
Podesta, Florencio Esteban
author_role author
author2 Moreno, Alejandra S.
Tripodi, Karina Eva Josefina
del Vecchio, Hernán A.
Meier, Guillermo
Bello, Fernando
Cocco, Mariángeles
Vázquez, Daniel
Podesta, Florencio Esteban
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citrus
Proteomics
Metabolomics
Postharvest
topic Citrus
Proteomics
Metabolomics
Postharvest
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15446
Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo; et al.; Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage; Elsevier Science; Postharvest Biology And Technology; 99; 1-2015; 80-87
0925-5214
url http://hdl.handle.net/11336/15446
identifier_str_mv Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo; et al.; Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage; Elsevier Science; Postharvest Biology And Technology; 99; 1-2015; 80-87
0925-5214
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2014.08.007
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925521414002415
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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