Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage
- Autores
- Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo; Bello, Fernando; Cocco, Mariángeles; Vázquez, Daniel; Podesta, Florencio Esteban
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina - Materia
-
Citrus
Proteomics
Metabolomics
Postharvest - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/15446
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Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storagePerotti, Valeria ElisaMoreno, Alejandra S.Tripodi, Karina Eva Josefinadel Vecchio, Hernán A.Meier, GuillermoBello, FernandoCocco, MariángelesVázquez, DanielPodesta, Florencio EstebanCitrusProteomicsMetabolomicsPostharvesthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue.Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaElsevier Science2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15446Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo; et al.; Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage; Elsevier Science; Postharvest Biology And Technology; 99; 1-2015; 80-870925-5214enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2014.08.007info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925521414002415info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:06Zoai:ri.conicet.gov.ar:11336/15446instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:06.924CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
title |
Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
spellingShingle |
Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage Perotti, Valeria Elisa Citrus Proteomics Metabolomics Postharvest |
title_short |
Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
title_full |
Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
title_fullStr |
Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
title_full_unstemmed |
Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
title_sort |
Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage |
dc.creator.none.fl_str_mv |
Perotti, Valeria Elisa Moreno, Alejandra S. Tripodi, Karina Eva Josefina del Vecchio, Hernán A. Meier, Guillermo Bello, Fernando Cocco, Mariángeles Vázquez, Daniel Podesta, Florencio Esteban |
author |
Perotti, Valeria Elisa |
author_facet |
Perotti, Valeria Elisa Moreno, Alejandra S. Tripodi, Karina Eva Josefina del Vecchio, Hernán A. Meier, Guillermo Bello, Fernando Cocco, Mariángeles Vázquez, Daniel Podesta, Florencio Esteban |
author_role |
author |
author2 |
Moreno, Alejandra S. Tripodi, Karina Eva Josefina del Vecchio, Hernán A. Meier, Guillermo Bello, Fernando Cocco, Mariángeles Vázquez, Daniel Podesta, Florencio Esteban |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Citrus Proteomics Metabolomics Postharvest |
topic |
Citrus Proteomics Metabolomics Postharvest |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue. Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: del Vecchio, Hernán A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Meier, Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Cocco, Mariángeles. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Vázquez, Daniel. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina |
description |
Heat treatment is a powerful and eco-friendly method to prevent Penicillium infection in citrus fruit during the postharvest. Several studies have been dedicated to investigate the general chemical changes that justify the immediate reaction responsible for the induced tolerance; but just how primary metabolism and enzymology are affected by heat treatment and along a prolonged cold storage is still unclear. In this work, the main enzymes of carbon metabolism of Valencia orange flavedo were analyzed during the postharvest period after a heat treatment (HT) of 48 h at 37 °C. Enzymatic activity measurements indicated that the NADPH producing enzymes glucose 6-phosphate dehydrogenase and non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase have lower levels in HT fruit. In parallel, a higher synthesis of sucrose from organic acids was observed in HT epicarp. Sucrose-phosphate synthase would have an important role in sucrose accumulation. The pathway of carbon through glycolysis was affected by cold storage, independently of HT, in a way that it favors the ATP-dependent phosphofructokinase over the PPi-dependent homologous enzyme and the use of phosphoenolpyruvate (PEP) by PEP carboxylase instead of pyruvate kinase. Similarly, phenylpropanoid compounds did not show major changes in response to HT, although some of them showed a marked descent along the cold storage. Proteomic studies revealed alterations in the abundance of ascorbate peroxidase, two germin-like proteins and small HSPs, completing the description of the main metabolic changes in this tissue. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/15446 Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo; et al.; Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage; Elsevier Science; Postharvest Biology And Technology; 99; 1-2015; 80-87 0925-5214 |
url |
http://hdl.handle.net/11336/15446 |
identifier_str_mv |
Perotti, Valeria Elisa; Moreno, Alejandra S.; Tripodi, Karina Eva Josefina; del Vecchio, Hernán A.; Meier, Guillermo; et al.; Biochemical characterization of the flavedo of heat-treated Valencia orange during postharvest cold storage; Elsevier Science; Postharvest Biology And Technology; 99; 1-2015; 80-87 0925-5214 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2014.08.007 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925521414002415 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614198071394304 |
score |
13.070432 |