Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds

Autores
Lescano Lopez, Carlos Ignacio; Torres, José Roberto; Cecchini, Nicolás Miguel; Alvarez, María Elena
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil and 5-bromouracil. To date, the involvement of MBD4L in plant physiological processes has not been analyzed. To address this, we studied the enzyme functions in seeds. We found that imbibition induced the MBD4L gene expression by generating two alternative transcripts, MBD4L.3 and MBD4L.4. Gene activation was stronger in aged than in non-aged seeds. Seeds from mbd4l-1 mutants displayed germination failures when maintained under control or ageing conditions, while 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 seeds reversed these phenotypes. Seed nuclear DNA repair, assessed by comet assays, was exacerbated in an MBD4L-dependent manner at 24 h post-imbibition. Under this condition, the BER genes ARP, APE1L, and LIG1 showed higher expression in 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 than in mbd4l-1 seeds, suggesting that these components could coordinate with MBD4L to repair damaged DNA bases in seeds. Interestingly, the ATM, ATR, BRCA1, RAD51, and WEE1 genes associated with the DNA damage response (DDR) pathway were activated in mbd4l-1, but not in 35S:MBD4L.3/mbd4l-1 or 35S:MBD4L.4/mbd4l-1 seeds. These results indicate that MBD4L is a key enzyme of a BER cascade that operates during seed imbibition, whose deficiency would cause genomic damage detected by DDR, generating a delay or reduction in germination.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Lescano Lopez, Carlos Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lescano Lopez, Carlos Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lescano Lopez, Carlos Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Torres, José Roberto. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, María Elena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
The Plant Journal : 1-12 (First published: 04 July 2024)
Materia
Seed Germination
DNA Damage
Germinación de las Semillas
Daño al ADN
Base Excision Repair
DNA Glycosylases
Seed Ageing
DNA Damage Responses
MBD4L
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18506
Acceder
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oai_identifier_str oai:localhost:20.500.12123/18506
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Arabidopsis DNA glycosylase MBD4L improves recovery of aged seedsLescano Lopez, Carlos IgnacioTorres, José RobertoCecchini, Nicolás MiguelAlvarez, María ElenaSeed GerminationDNA DamageGerminación de las SemillasDaño al ADNBase Excision RepairDNA GlycosylasesSeed AgeingDNA Damage ResponsesMBD4LDNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil and 5-bromouracil. To date, the involvement of MBD4L in plant physiological processes has not been analyzed. To address this, we studied the enzyme functions in seeds. We found that imbibition induced the MBD4L gene expression by generating two alternative transcripts, MBD4L.3 and MBD4L.4. Gene activation was stronger in aged than in non-aged seeds. Seeds from mbd4l-1 mutants displayed germination failures when maintained under control or ageing conditions, while 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 seeds reversed these phenotypes. Seed nuclear DNA repair, assessed by comet assays, was exacerbated in an MBD4L-dependent manner at 24 h post-imbibition. Under this condition, the BER genes ARP, APE1L, and LIG1 showed higher expression in 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 than in mbd4l-1 seeds, suggesting that these components could coordinate with MBD4L to repair damaged DNA bases in seeds. Interestingly, the ATM, ATR, BRCA1, RAD51, and WEE1 genes associated with the DNA damage response (DDR) pathway were activated in mbd4l-1, but not in 35S:MBD4L.3/mbd4l-1 or 35S:MBD4L.4/mbd4l-1 seeds. These results indicate that MBD4L is a key enzyme of a BER cascade that operates during seed imbibition, whose deficiency would cause genomic damage detected by DDR, generating a delay or reduction in germination.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Lescano Lopez, Carlos Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Lescano Lopez, Carlos Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lescano Lopez, Carlos Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Torres, José Roberto. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez, María Elena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2024-07-15T11:40:58Z2024-07-15T11:40:58Z2024-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18506https://onlinelibrary.wiley.com/doi/10.1111/tpj.169070960-74121365-313X (online)https://doi.org/10.1111/tpj.16907The Plant Journal : 1-12 (First published: 04 July 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:39Zoai:localhost:20.500.12123/18506instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:39.675INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
title Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
spellingShingle Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
Lescano Lopez, Carlos Ignacio
Seed Germination
DNA Damage
Germinación de las Semillas
Daño al ADN
Base Excision Repair
DNA Glycosylases
Seed Ageing
DNA Damage Responses
MBD4L
title_short Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
title_full Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
title_fullStr Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
title_full_unstemmed Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
title_sort Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
dc.creator.none.fl_str_mv Lescano Lopez, Carlos Ignacio
Torres, José Roberto
Cecchini, Nicolás Miguel
Alvarez, María Elena
author Lescano Lopez, Carlos Ignacio
author_facet Lescano Lopez, Carlos Ignacio
Torres, José Roberto
Cecchini, Nicolás Miguel
Alvarez, María Elena
author_role author
author2 Torres, José Roberto
Cecchini, Nicolás Miguel
Alvarez, María Elena
author2_role author
author
author
dc.subject.none.fl_str_mv Seed Germination
DNA Damage
Germinación de las Semillas
Daño al ADN
Base Excision Repair
DNA Glycosylases
Seed Ageing
DNA Damage Responses
MBD4L
topic Seed Germination
DNA Damage
Germinación de las Semillas
Daño al ADN
Base Excision Repair
DNA Glycosylases
Seed Ageing
DNA Damage Responses
MBD4L
dc.description.none.fl_txt_mv DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil and 5-bromouracil. To date, the involvement of MBD4L in plant physiological processes has not been analyzed. To address this, we studied the enzyme functions in seeds. We found that imbibition induced the MBD4L gene expression by generating two alternative transcripts, MBD4L.3 and MBD4L.4. Gene activation was stronger in aged than in non-aged seeds. Seeds from mbd4l-1 mutants displayed germination failures when maintained under control or ageing conditions, while 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 seeds reversed these phenotypes. Seed nuclear DNA repair, assessed by comet assays, was exacerbated in an MBD4L-dependent manner at 24 h post-imbibition. Under this condition, the BER genes ARP, APE1L, and LIG1 showed higher expression in 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 than in mbd4l-1 seeds, suggesting that these components could coordinate with MBD4L to repair damaged DNA bases in seeds. Interestingly, the ATM, ATR, BRCA1, RAD51, and WEE1 genes associated with the DNA damage response (DDR) pathway were activated in mbd4l-1, but not in 35S:MBD4L.3/mbd4l-1 or 35S:MBD4L.4/mbd4l-1 seeds. These results indicate that MBD4L is a key enzyme of a BER cascade that operates during seed imbibition, whose deficiency would cause genomic damage detected by DDR, generating a delay or reduction in germination.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Lescano Lopez, Carlos Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Lescano Lopez, Carlos Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lescano Lopez, Carlos Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Torres, José Roberto. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, María Elena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil and 5-bromouracil. To date, the involvement of MBD4L in plant physiological processes has not been analyzed. To address this, we studied the enzyme functions in seeds. We found that imbibition induced the MBD4L gene expression by generating two alternative transcripts, MBD4L.3 and MBD4L.4. Gene activation was stronger in aged than in non-aged seeds. Seeds from mbd4l-1 mutants displayed germination failures when maintained under control or ageing conditions, while 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 seeds reversed these phenotypes. Seed nuclear DNA repair, assessed by comet assays, was exacerbated in an MBD4L-dependent manner at 24 h post-imbibition. Under this condition, the BER genes ARP, APE1L, and LIG1 showed higher expression in 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 than in mbd4l-1 seeds, suggesting that these components could coordinate with MBD4L to repair damaged DNA bases in seeds. Interestingly, the ATM, ATR, BRCA1, RAD51, and WEE1 genes associated with the DNA damage response (DDR) pathway were activated in mbd4l-1, but not in 35S:MBD4L.3/mbd4l-1 or 35S:MBD4L.4/mbd4l-1 seeds. These results indicate that MBD4L is a key enzyme of a BER cascade that operates during seed imbibition, whose deficiency would cause genomic damage detected by DDR, generating a delay or reduction in germination.
publishDate 2024
dc.date.none.fl_str_mv 2024-07-15T11:40:58Z
2024-07-15T11:40:58Z
2024-07-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18506
https://onlinelibrary.wiley.com/doi/10.1111/tpj.16907
0960-7412
1365-313X (online)
https://doi.org/10.1111/tpj.16907
url http://hdl.handle.net/20.500.12123/18506
https://onlinelibrary.wiley.com/doi/10.1111/tpj.16907
https://doi.org/10.1111/tpj.16907
identifier_str_mv 0960-7412
1365-313X (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv The Plant Journal : 1-12 (First published: 04 July 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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