Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
- Autores
- O'Rourke, Eyleen J.; Mathieu, Aurelie; Ielpi, Luis; Radicella, Juan Pablo
- Año de publicación
- 2003
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- One of the remarkable characteristics of Helicobacter pylori is the high genetic diversity it displays. Based on the genome sequencing results, the absence of certain DNA repair activities has been postulated to be one of the causes for the genetic variability of this pathogen. We explored the possible base excision repair (BER) pathways present in H. pylori. We analyzed the activities corresponding to the enzymes participating in the first two steps of the pathway, the DNA glycosylases, specific for each kind of base damage, and the endonuclease that cleaves the resulting abasic (AP) site. We review here the data on the repair of alkylating DNA damage and oxidized pyrimidines and present results on studies carried out on bacterial extracts and purified proteins for the other BER activities. The combined approaches allowed the identification of a 3-methyl adenine DNA glycosylase, an endonuclease III, a uracil glycosylase, an adenine DNA glycosylase specific for 8-oxoguanine/adenine base pairs, and an AP endonuclease activity. We also discuss the possible role of the host in the bacterial genetic variability and the potential appearance of new alleles that could influence H. pylori persistence.
Fil: O'Rourke, Eyleen J.. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Mathieu, Aurelie. Centre National de la Recherche Scientifique; Francia
Fil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Radicella, Juan Pablo. Centre National de la Recherche Scientifique; Francia - Materia
-
Base Excision Repair
Dna Glycosylase
Genetic Diversity
Helicobacter Pylori - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/45241
Ver los metadatos del registro completo
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Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .O'Rourke, Eyleen J.Mathieu, AurelieIelpi, LuisRadicella, Juan PabloBase Excision RepairDna GlycosylaseGenetic DiversityHelicobacter Pylorihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1One of the remarkable characteristics of Helicobacter pylori is the high genetic diversity it displays. Based on the genome sequencing results, the absence of certain DNA repair activities has been postulated to be one of the causes for the genetic variability of this pathogen. We explored the possible base excision repair (BER) pathways present in H. pylori. We analyzed the activities corresponding to the enzymes participating in the first two steps of the pathway, the DNA glycosylases, specific for each kind of base damage, and the endonuclease that cleaves the resulting abasic (AP) site. We review here the data on the repair of alkylating DNA damage and oxidized pyrimidines and present results on studies carried out on bacterial extracts and purified proteins for the other BER activities. The combined approaches allowed the identification of a 3-methyl adenine DNA glycosylase, an endonuclease III, a uracil glycosylase, an adenine DNA glycosylase specific for 8-oxoguanine/adenine base pairs, and an AP endonuclease activity. We also discuss the possible role of the host in the bacterial genetic variability and the potential appearance of new alleles that could influence H. pylori persistence.Fil: O'Rourke, Eyleen J.. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Mathieu, Aurelie. Centre National de la Recherche Scientifique; FranciaFil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Radicella, Juan Pablo. Centre National de la Recherche Scientifique; FranciaAmerican Scientific Publishers2003-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45241O'Rourke, Eyleen J.; Mathieu, Aurelie; Ielpi, Luis; Radicella, Juan Pablo; Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .; American Scientific Publishers; Genome Letters; 2; 1-2; 3-2003; 41-471537-30531537-3053CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/glet/2003/00000002/F0020001/art00008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:08Zoai:ri.conicet.gov.ar:11336/45241instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:08.522CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori . |
title |
Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori . |
spellingShingle |
Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori . O'Rourke, Eyleen J. Base Excision Repair Dna Glycosylase Genetic Diversity Helicobacter Pylori |
title_short |
Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori . |
title_full |
Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori . |
title_fullStr |
Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori . |
title_full_unstemmed |
Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori . |
title_sort |
Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori . |
dc.creator.none.fl_str_mv |
O'Rourke, Eyleen J. Mathieu, Aurelie Ielpi, Luis Radicella, Juan Pablo |
author |
O'Rourke, Eyleen J. |
author_facet |
O'Rourke, Eyleen J. Mathieu, Aurelie Ielpi, Luis Radicella, Juan Pablo |
author_role |
author |
author2 |
Mathieu, Aurelie Ielpi, Luis Radicella, Juan Pablo |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Base Excision Repair Dna Glycosylase Genetic Diversity Helicobacter Pylori |
topic |
Base Excision Repair Dna Glycosylase Genetic Diversity Helicobacter Pylori |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
One of the remarkable characteristics of Helicobacter pylori is the high genetic diversity it displays. Based on the genome sequencing results, the absence of certain DNA repair activities has been postulated to be one of the causes for the genetic variability of this pathogen. We explored the possible base excision repair (BER) pathways present in H. pylori. We analyzed the activities corresponding to the enzymes participating in the first two steps of the pathway, the DNA glycosylases, specific for each kind of base damage, and the endonuclease that cleaves the resulting abasic (AP) site. We review here the data on the repair of alkylating DNA damage and oxidized pyrimidines and present results on studies carried out on bacterial extracts and purified proteins for the other BER activities. The combined approaches allowed the identification of a 3-methyl adenine DNA glycosylase, an endonuclease III, a uracil glycosylase, an adenine DNA glycosylase specific for 8-oxoguanine/adenine base pairs, and an AP endonuclease activity. We also discuss the possible role of the host in the bacterial genetic variability and the potential appearance of new alleles that could influence H. pylori persistence. Fil: O'Rourke, Eyleen J.. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Mathieu, Aurelie. Centre National de la Recherche Scientifique; Francia Fil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Radicella, Juan Pablo. Centre National de la Recherche Scientifique; Francia |
description |
One of the remarkable characteristics of Helicobacter pylori is the high genetic diversity it displays. Based on the genome sequencing results, the absence of certain DNA repair activities has been postulated to be one of the causes for the genetic variability of this pathogen. We explored the possible base excision repair (BER) pathways present in H. pylori. We analyzed the activities corresponding to the enzymes participating in the first two steps of the pathway, the DNA glycosylases, specific for each kind of base damage, and the endonuclease that cleaves the resulting abasic (AP) site. We review here the data on the repair of alkylating DNA damage and oxidized pyrimidines and present results on studies carried out on bacterial extracts and purified proteins for the other BER activities. The combined approaches allowed the identification of a 3-methyl adenine DNA glycosylase, an endonuclease III, a uracil glycosylase, an adenine DNA glycosylase specific for 8-oxoguanine/adenine base pairs, and an AP endonuclease activity. We also discuss the possible role of the host in the bacterial genetic variability and the potential appearance of new alleles that could influence H. pylori persistence. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/45241 O'Rourke, Eyleen J.; Mathieu, Aurelie; Ielpi, Luis; Radicella, Juan Pablo; Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .; American Scientific Publishers; Genome Letters; 2; 1-2; 3-2003; 41-47 1537-3053 1537-3053 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/45241 |
identifier_str_mv |
O'Rourke, Eyleen J.; Mathieu, Aurelie; Ielpi, Luis; Radicella, Juan Pablo; Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .; American Scientific Publishers; Genome Letters; 2; 1-2; 3-2003; 41-47 1537-3053 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/glet/2003/00000002/F0020001/art00008 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Scientific Publishers |
publisher.none.fl_str_mv |
American Scientific Publishers |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614454030893056 |
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13.070432 |