Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .

Autores
O'Rourke, Eyleen J.; Mathieu, Aurelie; Ielpi, Luis; Radicella, Juan Pablo
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the remarkable characteristics of Helicobacter pylori is the high genetic diversity it displays. Based on the genome sequencing results, the absence of certain DNA repair activities has been postulated to be one of the causes for the genetic variability of this pathogen. We explored the possible base excision repair (BER) pathways present in H. pylori. We analyzed the activities corresponding to the enzymes participating in the first two steps of the pathway, the DNA glycosylases, specific for each kind of base damage, and the endonuclease that cleaves the resulting abasic (AP) site. We review here the data on the repair of alkylating DNA damage and oxidized pyrimidines and present results on studies carried out on bacterial extracts and purified proteins for the other BER activities. The combined approaches allowed the identification of a 3-methyl adenine DNA glycosylase, an endonuclease III, a uracil glycosylase, an adenine DNA glycosylase specific for 8-oxoguanine/adenine base pairs, and an AP endonuclease activity. We also discuss the possible role of the host in the bacterial genetic variability and the potential appearance of new alleles that could influence H. pylori persistence.
Fil: O'Rourke, Eyleen J.. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Mathieu, Aurelie. Centre National de la Recherche Scientifique; Francia
Fil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Radicella, Juan Pablo. Centre National de la Recherche Scientifique; Francia
Materia
Base Excision Repair
Dna Glycosylase
Genetic Diversity
Helicobacter Pylori
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45241
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/45241
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network_name_str CONICET Digital (CONICET)
spelling Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .O'Rourke, Eyleen J.Mathieu, AurelieIelpi, LuisRadicella, Juan PabloBase Excision RepairDna GlycosylaseGenetic DiversityHelicobacter Pylorihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1One of the remarkable characteristics of Helicobacter pylori is the high genetic diversity it displays. Based on the genome sequencing results, the absence of certain DNA repair activities has been postulated to be one of the causes for the genetic variability of this pathogen. We explored the possible base excision repair (BER) pathways present in H. pylori. We analyzed the activities corresponding to the enzymes participating in the first two steps of the pathway, the DNA glycosylases, specific for each kind of base damage, and the endonuclease that cleaves the resulting abasic (AP) site. We review here the data on the repair of alkylating DNA damage and oxidized pyrimidines and present results on studies carried out on bacterial extracts and purified proteins for the other BER activities. The combined approaches allowed the identification of a 3-methyl adenine DNA glycosylase, an endonuclease III, a uracil glycosylase, an adenine DNA glycosylase specific for 8-oxoguanine/adenine base pairs, and an AP endonuclease activity. We also discuss the possible role of the host in the bacterial genetic variability and the potential appearance of new alleles that could influence H. pylori persistence.Fil: O'Rourke, Eyleen J.. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Mathieu, Aurelie. Centre National de la Recherche Scientifique; FranciaFil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Radicella, Juan Pablo. Centre National de la Recherche Scientifique; FranciaAmerican Scientific Publishers2003-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45241O'Rourke, Eyleen J.; Mathieu, Aurelie; Ielpi, Luis; Radicella, Juan Pablo; Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .; American Scientific Publishers; Genome Letters; 2; 1-2; 3-2003; 41-471537-30531537-3053CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/glet/2003/00000002/F0020001/art00008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:40:06Zoai:ri.conicet.gov.ar:11336/45241instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:40:07.264CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
title Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
spellingShingle Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
O'Rourke, Eyleen J.
Base Excision Repair
Dna Glycosylase
Genetic Diversity
Helicobacter Pylori
title_short Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
title_full Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
title_fullStr Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
title_full_unstemmed Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
title_sort Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .
dc.creator.none.fl_str_mv O'Rourke, Eyleen J.
Mathieu, Aurelie
Ielpi, Luis
Radicella, Juan Pablo
author O'Rourke, Eyleen J.
author_facet O'Rourke, Eyleen J.
Mathieu, Aurelie
Ielpi, Luis
Radicella, Juan Pablo
author_role author
author2 Mathieu, Aurelie
Ielpi, Luis
Radicella, Juan Pablo
author2_role author
author
author
dc.subject.none.fl_str_mv Base Excision Repair
Dna Glycosylase
Genetic Diversity
Helicobacter Pylori
topic Base Excision Repair
Dna Glycosylase
Genetic Diversity
Helicobacter Pylori
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv One of the remarkable characteristics of Helicobacter pylori is the high genetic diversity it displays. Based on the genome sequencing results, the absence of certain DNA repair activities has been postulated to be one of the causes for the genetic variability of this pathogen. We explored the possible base excision repair (BER) pathways present in H. pylori. We analyzed the activities corresponding to the enzymes participating in the first two steps of the pathway, the DNA glycosylases, specific for each kind of base damage, and the endonuclease that cleaves the resulting abasic (AP) site. We review here the data on the repair of alkylating DNA damage and oxidized pyrimidines and present results on studies carried out on bacterial extracts and purified proteins for the other BER activities. The combined approaches allowed the identification of a 3-methyl adenine DNA glycosylase, an endonuclease III, a uracil glycosylase, an adenine DNA glycosylase specific for 8-oxoguanine/adenine base pairs, and an AP endonuclease activity. We also discuss the possible role of the host in the bacterial genetic variability and the potential appearance of new alleles that could influence H. pylori persistence.
Fil: O'Rourke, Eyleen J.. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Mathieu, Aurelie. Centre National de la Recherche Scientifique; Francia
Fil: Ielpi, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Radicella, Juan Pablo. Centre National de la Recherche Scientifique; Francia
description One of the remarkable characteristics of Helicobacter pylori is the high genetic diversity it displays. Based on the genome sequencing results, the absence of certain DNA repair activities has been postulated to be one of the causes for the genetic variability of this pathogen. We explored the possible base excision repair (BER) pathways present in H. pylori. We analyzed the activities corresponding to the enzymes participating in the first two steps of the pathway, the DNA glycosylases, specific for each kind of base damage, and the endonuclease that cleaves the resulting abasic (AP) site. We review here the data on the repair of alkylating DNA damage and oxidized pyrimidines and present results on studies carried out on bacterial extracts and purified proteins for the other BER activities. The combined approaches allowed the identification of a 3-methyl adenine DNA glycosylase, an endonuclease III, a uracil glycosylase, an adenine DNA glycosylase specific for 8-oxoguanine/adenine base pairs, and an AP endonuclease activity. We also discuss the possible role of the host in the bacterial genetic variability and the potential appearance of new alleles that could influence H. pylori persistence.
publishDate 2003
dc.date.none.fl_str_mv 2003-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45241
O'Rourke, Eyleen J.; Mathieu, Aurelie; Ielpi, Luis; Radicella, Juan Pablo; Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .; American Scientific Publishers; Genome Letters; 2; 1-2; 3-2003; 41-47
1537-3053
1537-3053
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45241
identifier_str_mv O'Rourke, Eyleen J.; Mathieu, Aurelie; Ielpi, Luis; Radicella, Juan Pablo; Genetic variability and DNA repair: base excision repair activities in Helicobacter pylori .; American Scientific Publishers; Genome Letters; 2; 1-2; 3-2003; 41-47
1537-3053
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/asp/glet/2003/00000002/F0020001/art00008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Scientific Publishers
publisher.none.fl_str_mv American Scientific Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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