Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests

Autores
Sauka, Diego Herman; Peralta, Cecilia; Pérez, Melisa Paula; Onco, Maria Ines; Fiodor, Angelika; Caballero, Javier; Caballero, Primitivo; Berry, Colin; Del Valle, Eleodoro Eduardo; Palma, Leopoldo
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Bacillus cereus group includes eight species: Bacillus anthracis, B. cereus, Bacillus cytotoxicus, Bacillus mycoides, Bacillus pseudomycoides, Bacillus thuringiensis, Bacillus weihenstephanensis and Bacillus toyonensis, which are highly related at the phylogenetic level. In this work, we performed the isolation and characterization of a novel Bacillus sp. strain exhibiting parasporal crystals with insecticidal activity that was initially classified as a Bacillus thuringiensis strain. Its genome encoded three genes showing homology to known pesticidal proteins from B. thuringiensis and Lysinibacillus sphaericus proteins: Cry7Ga1 (a crystal protein), a Mpp2Aa3 (ETX/Mtx2 family) homolog and a mosquitocidal-like protein (NPP1). However, since its genome sequence shared > 98% ANI with several Bacillus toyonensis genomes, the strain has subsequently been renamed as Bacillus toyonensis biovar Thuringiensis and designated Bto-UNVM_94. Bioassays demonstrated that this novel strain exhibited toxicity against Cydia pomonella (Lepidoptera: Tortricidae) and Anthonomus grandis (Coleoptera: Curculionidae), a low toxicity against Aedes aegypti (Diptera: Culicidae) and Alphitobius diaperinus (Coleoptera: Tenebrionidae) whereas no toxicity was shown to the free-living nematode Panagrellus redivivus (Rhabditida: Panagrolaimidae).
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Villa María (CIT-VM). Universidad Nacional de Villa María; Argentina
Fil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Onco, María Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Fiodor, Angelika. University of Warsaw. Faculty of Biology. Institute of Microbiology. Department of Environmental Microbiology and Biotechnology; Polonia
Fil: Caballero, Javier. Universidad Pública de Navarra, Institute for Multidisciplinary Research in Applied Biology; España
Fil: Caballero, Javier. Bioinsectis. Departamento de Investigación y Desarrollo; España
Fil: Caballero, Primitivo. Universidad Pública de Navarra, Institute for Multidisciplinary Research in Applied Biology; España
Fil: Caballero, Primitivo., Bioinsectis. Departamento de Investigación y Desarrollo; España
Fil: Berry, Colin. Cardiff University. Cardiff School of Biosciences; Reino Unido
Fil: del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Palma, Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Palma, Leopoldo. Universitat de Valéncia. Department of Genetics. Instituto de Biotecnología y Biomedicina (BIOTECMED); España
Fuente
Biological Control 167 : 104838 (abril 2022)
Materia
Bacillus
Bacillus thuringiensis
Entomogenous Bacteria
Bacterias Entomógenas
Bacillus cereus
Bacillus toyonensis
Entomopathogenic Bacteria
Biovar Thuringiensis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12409
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oai_identifier_str oai:localhost:20.500.12123/12409
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network_name_str INTA Digital (INTA)
spelling Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pestsSauka, Diego HermanPeralta, CeciliaPérez, Melisa PaulaOnco, Maria InesFiodor, AngelikaCaballero, JavierCaballero, PrimitivoBerry, ColinDel Valle, Eleodoro EduardoPalma, LeopoldoBacillusBacillus thuringiensisEntomogenous BacteriaBacterias EntomógenasBacillus cereusBacillus toyonensisEntomopathogenic BacteriaBiovar ThuringiensisThe Bacillus cereus group includes eight species: Bacillus anthracis, B. cereus, Bacillus cytotoxicus, Bacillus mycoides, Bacillus pseudomycoides, Bacillus thuringiensis, Bacillus weihenstephanensis and Bacillus toyonensis, which are highly related at the phylogenetic level. In this work, we performed the isolation and characterization of a novel Bacillus sp. strain exhibiting parasporal crystals with insecticidal activity that was initially classified as a Bacillus thuringiensis strain. Its genome encoded three genes showing homology to known pesticidal proteins from B. thuringiensis and Lysinibacillus sphaericus proteins: Cry7Ga1 (a crystal protein), a Mpp2Aa3 (ETX/Mtx2 family) homolog and a mosquitocidal-like protein (NPP1). However, since its genome sequence shared > 98% ANI with several Bacillus toyonensis genomes, the strain has subsequently been renamed as Bacillus toyonensis biovar Thuringiensis and designated Bto-UNVM_94. Bioassays demonstrated that this novel strain exhibited toxicity against Cydia pomonella (Lepidoptera: Tortricidae) and Anthonomus grandis (Coleoptera: Curculionidae), a low toxicity against Aedes aegypti (Diptera: Culicidae) and Alphitobius diaperinus (Coleoptera: Tenebrionidae) whereas no toxicity was shown to the free-living nematode Panagrellus redivivus (Rhabditida: Panagrolaimidae).Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; ArgentinaFil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Villa María (CIT-VM). Universidad Nacional de Villa María; ArgentinaFil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; ArgentinaFil: Onco, María Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; ArgentinaFil: Fiodor, Angelika. University of Warsaw. Faculty of Biology. Institute of Microbiology. Department of Environmental Microbiology and Biotechnology; PoloniaFil: Caballero, Javier. Universidad Pública de Navarra, Institute for Multidisciplinary Research in Applied Biology; EspañaFil: Caballero, Javier. Bioinsectis. Departamento de Investigación y Desarrollo; EspañaFil: Caballero, Primitivo. Universidad Pública de Navarra, Institute for Multidisciplinary Research in Applied Biology; EspañaFil: Caballero, Primitivo., Bioinsectis. Departamento de Investigación y Desarrollo; EspañaFil: Berry, Colin. Cardiff University. Cardiff School of Biosciences; Reino UnidoFil: del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; ArgentinaFil: Palma, Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; ArgentinaFil: Palma, Leopoldo. Universitat de Valéncia. Department of Genetics. Instituto de Biotecnología y Biomedicina (BIOTECMED); EspañaElsevier2022-07-26T22:26:07Z2022-07-26T22:26:07Z2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12409https://www.sciencedirect.com/science/article/abs/pii/S10499644220000321049-9644https://doi.org/10.1016/j.biocontrol.2022.104838Biological Control 167 : 104838 (abril 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetalinfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:28Zoai:localhost:20.500.12123/12409instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:28.497INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests
title Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests
spellingShingle Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests
Sauka, Diego Herman
Bacillus
Bacillus thuringiensis
Entomogenous Bacteria
Bacterias Entomógenas
Bacillus cereus
Bacillus toyonensis
Entomopathogenic Bacteria
Biovar Thuringiensis
title_short Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests
title_full Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests
title_fullStr Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests
title_full_unstemmed Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests
title_sort Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests
dc.creator.none.fl_str_mv Sauka, Diego Herman
Peralta, Cecilia
Pérez, Melisa Paula
Onco, Maria Ines
Fiodor, Angelika
Caballero, Javier
Caballero, Primitivo
Berry, Colin
Del Valle, Eleodoro Eduardo
Palma, Leopoldo
author Sauka, Diego Herman
author_facet Sauka, Diego Herman
Peralta, Cecilia
Pérez, Melisa Paula
Onco, Maria Ines
Fiodor, Angelika
Caballero, Javier
Caballero, Primitivo
Berry, Colin
Del Valle, Eleodoro Eduardo
Palma, Leopoldo
author_role author
author2 Peralta, Cecilia
Pérez, Melisa Paula
Onco, Maria Ines
Fiodor, Angelika
Caballero, Javier
Caballero, Primitivo
Berry, Colin
Del Valle, Eleodoro Eduardo
Palma, Leopoldo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacillus
Bacillus thuringiensis
Entomogenous Bacteria
Bacterias Entomógenas
Bacillus cereus
Bacillus toyonensis
Entomopathogenic Bacteria
Biovar Thuringiensis
topic Bacillus
Bacillus thuringiensis
Entomogenous Bacteria
Bacterias Entomógenas
Bacillus cereus
Bacillus toyonensis
Entomopathogenic Bacteria
Biovar Thuringiensis
dc.description.none.fl_txt_mv The Bacillus cereus group includes eight species: Bacillus anthracis, B. cereus, Bacillus cytotoxicus, Bacillus mycoides, Bacillus pseudomycoides, Bacillus thuringiensis, Bacillus weihenstephanensis and Bacillus toyonensis, which are highly related at the phylogenetic level. In this work, we performed the isolation and characterization of a novel Bacillus sp. strain exhibiting parasporal crystals with insecticidal activity that was initially classified as a Bacillus thuringiensis strain. Its genome encoded three genes showing homology to known pesticidal proteins from B. thuringiensis and Lysinibacillus sphaericus proteins: Cry7Ga1 (a crystal protein), a Mpp2Aa3 (ETX/Mtx2 family) homolog and a mosquitocidal-like protein (NPP1). However, since its genome sequence shared > 98% ANI with several Bacillus toyonensis genomes, the strain has subsequently been renamed as Bacillus toyonensis biovar Thuringiensis and designated Bto-UNVM_94. Bioassays demonstrated that this novel strain exhibited toxicity against Cydia pomonella (Lepidoptera: Tortricidae) and Anthonomus grandis (Coleoptera: Curculionidae), a low toxicity against Aedes aegypti (Diptera: Culicidae) and Alphitobius diaperinus (Coleoptera: Tenebrionidae) whereas no toxicity was shown to the free-living nematode Panagrellus redivivus (Rhabditida: Panagrolaimidae).
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Peralta, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Villa María (CIT-VM). Universidad Nacional de Villa María; Argentina
Fil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Onco, María Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Fiodor, Angelika. University of Warsaw. Faculty of Biology. Institute of Microbiology. Department of Environmental Microbiology and Biotechnology; Polonia
Fil: Caballero, Javier. Universidad Pública de Navarra, Institute for Multidisciplinary Research in Applied Biology; España
Fil: Caballero, Javier. Bioinsectis. Departamento de Investigación y Desarrollo; España
Fil: Caballero, Primitivo. Universidad Pública de Navarra, Institute for Multidisciplinary Research in Applied Biology; España
Fil: Caballero, Primitivo., Bioinsectis. Departamento de Investigación y Desarrollo; España
Fil: Berry, Colin. Cardiff University. Cardiff School of Biosciences; Reino Unido
Fil: del Valle, Eleodoro Eduardo. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina
Fil: Palma, Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Microbiología y Zoología Agrícola; Argentina
Fil: Palma, Leopoldo. Universitat de Valéncia. Department of Genetics. Instituto de Biotecnología y Biomedicina (BIOTECMED); España
description The Bacillus cereus group includes eight species: Bacillus anthracis, B. cereus, Bacillus cytotoxicus, Bacillus mycoides, Bacillus pseudomycoides, Bacillus thuringiensis, Bacillus weihenstephanensis and Bacillus toyonensis, which are highly related at the phylogenetic level. In this work, we performed the isolation and characterization of a novel Bacillus sp. strain exhibiting parasporal crystals with insecticidal activity that was initially classified as a Bacillus thuringiensis strain. Its genome encoded three genes showing homology to known pesticidal proteins from B. thuringiensis and Lysinibacillus sphaericus proteins: Cry7Ga1 (a crystal protein), a Mpp2Aa3 (ETX/Mtx2 family) homolog and a mosquitocidal-like protein (NPP1). However, since its genome sequence shared > 98% ANI with several Bacillus toyonensis genomes, the strain has subsequently been renamed as Bacillus toyonensis biovar Thuringiensis and designated Bto-UNVM_94. Bioassays demonstrated that this novel strain exhibited toxicity against Cydia pomonella (Lepidoptera: Tortricidae) and Anthonomus grandis (Coleoptera: Curculionidae), a low toxicity against Aedes aegypti (Diptera: Culicidae) and Alphitobius diaperinus (Coleoptera: Tenebrionidae) whereas no toxicity was shown to the free-living nematode Panagrellus redivivus (Rhabditida: Panagrolaimidae).
publishDate 2022
dc.date.none.fl_str_mv 2022-07-26T22:26:07Z
2022-07-26T22:26:07Z
2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12409
https://www.sciencedirect.com/science/article/abs/pii/S1049964422000032
1049-9644
https://doi.org/10.1016/j.biocontrol.2022.104838
url http://hdl.handle.net/20.500.12123/12409
https://www.sciencedirect.com/science/article/abs/pii/S1049964422000032
https://doi.org/10.1016/j.biocontrol.2022.104838
identifier_str_mv 1049-9644
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Biological Control 167 : 104838 (abril 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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