New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates

Autores
Sauka, Diego Hernan; Piccinetti, Carlos Fabian; Vallejo, Daniela Adriana; Onco, Maria Ines; Perez, Melisa Paula; Benintende, Graciela Beatriz
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phosphate-solubilizing strain INTA 293-1 from a soil sample of Corrientes province (Argentina) was identified as Bacillus thuringiensis based on its faculty to produce crystalline inclusions during sporulation and 16S rDNA sequence similarity. INTA 293-1 was found to produce bipyramidal and cuboidal crystals, containing major proteins of ca. 130 and 65 kDa respectively, resembling those present in Lepidoptera- and Diptera-active strains. Accordingly, INTA 293-1 showed insecticidal activity against lepidopteran and dipteran larvae. No insecticidal activity nor evident effects on the normal development of coleopteran larvae or nematodes were observed. Screening of cry, cyt and vip genes showed that INTA 293-1 contains cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa genes. Furthermore, this new entomopathogenic strain of B. thuringiensis was able to solubilize different sources of inorganic phosphates in liquid media following similar solubilization kinetics. INTA 293-1 was effective in solubilizing hydroxyapatite, patagonic phosphate rock, and to a lesser extent the aluminium phosphate. The differential behaviour between them may be explained by differences in INTA 293-1 survival. Phosphate solubilization was associated with pH reduction. This study reveals a little-explored aspect of B. thuringiensis and paves the way for the development of new agrobiological products with multiple modes of action.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Piccinetti, Carlos Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Vallejo, Daniela Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Onco, María Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología e Instituto de Biotecnología; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología; Argentina
Fuente
Applied Soil Ecology 160 : 103839 (April 2021)
Materia
Bacillus thuringiensis
Plagas de Plantas
Bacterias Entomógenas
Plant Pests
Pest Control
Entomogenous Bacteria
Control de Plagas
Bacterias solubilizadoras de fosfato
Bacterias Entomopatógenas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10827
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10827
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphatesSauka, Diego HernanPiccinetti, Carlos FabianVallejo, Daniela AdrianaOnco, Maria InesPerez, Melisa PaulaBenintende, Graciela BeatrizBacillus thuringiensisPlagas de PlantasBacterias EntomógenasPlant PestsPest ControlEntomogenous BacteriaControl de PlagasBacterias solubilizadoras de fosfatoBacterias EntomopatógenasPhosphate-solubilizing strain INTA 293-1 from a soil sample of Corrientes province (Argentina) was identified as Bacillus thuringiensis based on its faculty to produce crystalline inclusions during sporulation and 16S rDNA sequence similarity. INTA 293-1 was found to produce bipyramidal and cuboidal crystals, containing major proteins of ca. 130 and 65 kDa respectively, resembling those present in Lepidoptera- and Diptera-active strains. Accordingly, INTA 293-1 showed insecticidal activity against lepidopteran and dipteran larvae. No insecticidal activity nor evident effects on the normal development of coleopteran larvae or nematodes were observed. Screening of cry, cyt and vip genes showed that INTA 293-1 contains cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa genes. Furthermore, this new entomopathogenic strain of B. thuringiensis was able to solubilize different sources of inorganic phosphates in liquid media following similar solubilization kinetics. INTA 293-1 was effective in solubilizing hydroxyapatite, patagonic phosphate rock, and to a lesser extent the aluminium phosphate. The differential behaviour between them may be explained by differences in INTA 293-1 survival. Phosphate solubilization was associated with pH reduction. This study reveals a little-explored aspect of B. thuringiensis and paves the way for the development of new agrobiological products with multiple modes of action.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Piccinetti, Carlos Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Vallejo, Daniela Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Onco, María Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología e Instituto de Biotecnología; ArgentinaFil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología; ArgentinaElsevier2021-12-02T11:29:28Z2021-12-02T11:29:28Z2021-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10827https://www.sciencedirect.com/science/article/pii/S092913932030768X0929-1393https://doi.org/10.1016/j.apsoil.2020.103839Applied Soil Ecology 160 : 103839 (April 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNPV-1135033/AR./Desarrollo de herramientas para el manejo integrado de artrópodos perjudiciales.info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetalinfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:24Zoai:localhost:20.500.12123/10827instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:25.422INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
title New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
spellingShingle New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
Sauka, Diego Hernan
Bacillus thuringiensis
Plagas de Plantas
Bacterias Entomógenas
Plant Pests
Pest Control
Entomogenous Bacteria
Control de Plagas
Bacterias solubilizadoras de fosfato
Bacterias Entomopatógenas
title_short New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
title_full New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
title_fullStr New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
title_full_unstemmed New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
title_sort New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
dc.creator.none.fl_str_mv Sauka, Diego Hernan
Piccinetti, Carlos Fabian
Vallejo, Daniela Adriana
Onco, Maria Ines
Perez, Melisa Paula
Benintende, Graciela Beatriz
author Sauka, Diego Hernan
author_facet Sauka, Diego Hernan
Piccinetti, Carlos Fabian
Vallejo, Daniela Adriana
Onco, Maria Ines
Perez, Melisa Paula
Benintende, Graciela Beatriz
author_role author
author2 Piccinetti, Carlos Fabian
Vallejo, Daniela Adriana
Onco, Maria Ines
Perez, Melisa Paula
Benintende, Graciela Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacillus thuringiensis
Plagas de Plantas
Bacterias Entomógenas
Plant Pests
Pest Control
Entomogenous Bacteria
Control de Plagas
Bacterias solubilizadoras de fosfato
Bacterias Entomopatógenas
topic Bacillus thuringiensis
Plagas de Plantas
Bacterias Entomógenas
Plant Pests
Pest Control
Entomogenous Bacteria
Control de Plagas
Bacterias solubilizadoras de fosfato
Bacterias Entomopatógenas
dc.description.none.fl_txt_mv Phosphate-solubilizing strain INTA 293-1 from a soil sample of Corrientes province (Argentina) was identified as Bacillus thuringiensis based on its faculty to produce crystalline inclusions during sporulation and 16S rDNA sequence similarity. INTA 293-1 was found to produce bipyramidal and cuboidal crystals, containing major proteins of ca. 130 and 65 kDa respectively, resembling those present in Lepidoptera- and Diptera-active strains. Accordingly, INTA 293-1 showed insecticidal activity against lepidopteran and dipteran larvae. No insecticidal activity nor evident effects on the normal development of coleopteran larvae or nematodes were observed. Screening of cry, cyt and vip genes showed that INTA 293-1 contains cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa genes. Furthermore, this new entomopathogenic strain of B. thuringiensis was able to solubilize different sources of inorganic phosphates in liquid media following similar solubilization kinetics. INTA 293-1 was effective in solubilizing hydroxyapatite, patagonic phosphate rock, and to a lesser extent the aluminium phosphate. The differential behaviour between them may be explained by differences in INTA 293-1 survival. Phosphate solubilization was associated with pH reduction. This study reveals a little-explored aspect of B. thuringiensis and paves the way for the development of new agrobiological products with multiple modes of action.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Piccinetti, Carlos Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Vallejo, Daniela Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Onco, María Inés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Pérez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología e Instituto de Biotecnología; Argentina
Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología; Argentina
description Phosphate-solubilizing strain INTA 293-1 from a soil sample of Corrientes province (Argentina) was identified as Bacillus thuringiensis based on its faculty to produce crystalline inclusions during sporulation and 16S rDNA sequence similarity. INTA 293-1 was found to produce bipyramidal and cuboidal crystals, containing major proteins of ca. 130 and 65 kDa respectively, resembling those present in Lepidoptera- and Diptera-active strains. Accordingly, INTA 293-1 showed insecticidal activity against lepidopteran and dipteran larvae. No insecticidal activity nor evident effects on the normal development of coleopteran larvae or nematodes were observed. Screening of cry, cyt and vip genes showed that INTA 293-1 contains cry1Ac, cry1Ia, cry2Aa, cry2Ab and vip3Aa genes. Furthermore, this new entomopathogenic strain of B. thuringiensis was able to solubilize different sources of inorganic phosphates in liquid media following similar solubilization kinetics. INTA 293-1 was effective in solubilizing hydroxyapatite, patagonic phosphate rock, and to a lesser extent the aluminium phosphate. The differential behaviour between them may be explained by differences in INTA 293-1 survival. Phosphate solubilization was associated with pH reduction. This study reveals a little-explored aspect of B. thuringiensis and paves the way for the development of new agrobiological products with multiple modes of action.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-02T11:29:28Z
2021-12-02T11:29:28Z
2021-04-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10827
https://www.sciencedirect.com/science/article/pii/S092913932030768X
0929-1393
https://doi.org/10.1016/j.apsoil.2020.103839
url http://hdl.handle.net/20.500.12123/10827
https://www.sciencedirect.com/science/article/pii/S092913932030768X
https://doi.org/10.1016/j.apsoil.2020.103839
identifier_str_mv 0929-1393
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNPV-1135033/AR./Desarrollo de herramientas para el manejo integrado de artrópodos perjudiciales.
info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001/2019-PD-E4-I069-001/AR./Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Applied Soil Ecology 160 : 103839 (April 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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