Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis

Autores
Sauka, Diego Herman
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Presentación en diapositivas y resumen
Bacillus thuringiensis is a gram-positive bacterium which synthesizes insecticidal virulence factors. The most important are the Cry proteins, which are produced during sporulation as crystal parasporal inclusions. These proteins along with the spores constitute the active ingredients of the most widely used biological pesticide to control agricultural insect pests and vectors of human and animal diseases. Some strains are capable of also expressing other insecticidal proteins called Vip or Sip during the vegetative growing phase. All these virulence factors have in common that are very specific, exhibiting a narrow host range of species within an insect order. In contrast, in some B. thuringiensis strains there are other secreted virulence factors such as β- exotoxins, chitinases, haemolysins, and enterotoxins, unlike Cry, Vip and Sip proteins, that are unspecific. In our laboratory, different strategies based on phenotypic and genotypic criteria have been successfully used to identify B. thuringiensis virulence factors: toxicity bioassays, competition binding assays performed on brush border membrane vesicles of insects, PCR-based detection of insecticidal toxin genes and comparative genomics. The knowledge of the virulence factors present in a particular strain is a key in understanding pathogenesis and essential for the development of new bioinsecticides or Bt plants.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
VI Congreso Internacional y VIII Congreso Nacional de Investigación Científica y Tecnológica (CONINTI 2019). Ica, Perú. 06 a 09/11/2019
Materia
Control Biológico
Bacillus thuringiensis
Biological Control
Bacteria
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/19987
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spelling Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensisSauka, Diego HermanControl BiológicoBacillus thuringiensisBiological ControlBacteriaPresentación en diapositivas y resumenBacillus thuringiensis is a gram-positive bacterium which synthesizes insecticidal virulence factors. The most important are the Cry proteins, which are produced during sporulation as crystal parasporal inclusions. These proteins along with the spores constitute the active ingredients of the most widely used biological pesticide to control agricultural insect pests and vectors of human and animal diseases. Some strains are capable of also expressing other insecticidal proteins called Vip or Sip during the vegetative growing phase. All these virulence factors have in common that are very specific, exhibiting a narrow host range of species within an insect order. In contrast, in some B. thuringiensis strains there are other secreted virulence factors such as β- exotoxins, chitinases, haemolysins, and enterotoxins, unlike Cry, Vip and Sip proteins, that are unspecific. In our laboratory, different strategies based on phenotypic and genotypic criteria have been successfully used to identify B. thuringiensis virulence factors: toxicity bioassays, competition binding assays performed on brush border membrane vesicles of insects, PCR-based detection of insecticidal toxin genes and comparative genomics. The knowledge of the virulence factors present in a particular strain is a key in understanding pathogenesis and essential for the development of new bioinsecticides or Bt plants.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA); ArgentinaFil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaUniversidad Nacional San Luis Gonzaga (Perú)2024-10-30T12:42:00Z2024-10-30T12:42:00Z2019-09info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/20.500.12123/19987978-612-45365-6-4VI Congreso Internacional y VIII Congreso Nacional de Investigación Científica y Tecnológica (CONINTI 2019). Ica, Perú. 06 a 09/11/2019reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:12Zoai:localhost:20.500.12123/19987instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:12.476INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis
title Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis
spellingShingle Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis
Sauka, Diego Herman
Control Biológico
Bacillus thuringiensis
Biological Control
Bacteria
title_short Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis
title_full Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis
title_fullStr Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis
title_full_unstemmed Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis
title_sort Strategies for the identification of Bacillus thuringiensis virulence factors = Estrategias para la identificación de factores de virulencia de Bacillus thuringiensis
dc.creator.none.fl_str_mv Sauka, Diego Herman
author Sauka, Diego Herman
author_facet Sauka, Diego Herman
author_role author
dc.subject.none.fl_str_mv Control Biológico
Bacillus thuringiensis
Biological Control
Bacteria
topic Control Biológico
Bacillus thuringiensis
Biological Control
Bacteria
dc.description.none.fl_txt_mv Presentación en diapositivas y resumen
Bacillus thuringiensis is a gram-positive bacterium which synthesizes insecticidal virulence factors. The most important are the Cry proteins, which are produced during sporulation as crystal parasporal inclusions. These proteins along with the spores constitute the active ingredients of the most widely used biological pesticide to control agricultural insect pests and vectors of human and animal diseases. Some strains are capable of also expressing other insecticidal proteins called Vip or Sip during the vegetative growing phase. All these virulence factors have in common that are very specific, exhibiting a narrow host range of species within an insect order. In contrast, in some B. thuringiensis strains there are other secreted virulence factors such as β- exotoxins, chitinases, haemolysins, and enterotoxins, unlike Cry, Vip and Sip proteins, that are unspecific. In our laboratory, different strategies based on phenotypic and genotypic criteria have been successfully used to identify B. thuringiensis virulence factors: toxicity bioassays, competition binding assays performed on brush border membrane vesicles of insects, PCR-based detection of insecticidal toxin genes and comparative genomics. The knowledge of the virulence factors present in a particular strain is a key in understanding pathogenesis and essential for the development of new bioinsecticides or Bt plants.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Sauka, Diego Herman. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMYZA); Argentina
Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Presentación en diapositivas y resumen
publishDate 2019
dc.date.none.fl_str_mv 2019-09
2024-10-30T12:42:00Z
2024-10-30T12:42:00Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/19987
978-612-45365-6-4
url http://hdl.handle.net/20.500.12123/19987
identifier_str_mv 978-612-45365-6-4
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional San Luis Gonzaga (Perú)
publisher.none.fl_str_mv Universidad Nacional San Luis Gonzaga (Perú)
dc.source.none.fl_str_mv VI Congreso Internacional y VIII Congreso Nacional de Investigación Científica y Tecnológica (CONINTI 2019). Ica, Perú. 06 a 09/11/2019
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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